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321.
To alleviate toxicological effect induced by cadmium in mungbean seedlings, seeds were divided into four groups: The controls
groups (CK, without treatment), magnetic field treated groups (MF), cadmium treated groups (CS), and magnetic field treated
followed by cadmium treated groups (MF + CS).The results showed: (i) Compared with the controls, cadmium stress resulted in
enhancing in the concentration of malondialdehyde, H2O2 and O2−, and the conductivity of electrolyte leakage while decreasing in the nitrice oxide synthase (NOS) activity, the concentration
of nitrice oxide (NO), chlorophyll and total carbon and nitrogen, the net photosynthetic rate, the stomatal conductance, the
transpiration rate, the water use efficiency, the lateral number and seedlings growth except for intercellular CO2 concentration increase. However, the seedlings treated with 600 mT magnetic field followed by cadmium stress the concentration
of malondialdehyde, H2O2 and O2−, and the conductivity of electrolyte leakage decreased, while the above mentioned NO concentration, NOS activity, photosynthesis
and growth parameters increased compared to cadmium stress alone. (ii) Compared with the cadmium stress (CS), the seedling
growth were inhibited when the seeds were treated with NO scavenger (hemoglobin, HB) and inhibition of NO generating enzyme
(sodium tungstate, ST), conversely, the seedling growth were improved by the NO donor sodium nitroprusside (SNP) and CaCl2. In the case of the HB and ST treatment followed by magnetic field and then the seedling subjected to CS, the seedlings growth
was better than that of hemoglobin (HB) followed by CS and ST followed by CS. The seeds were treated with SNP and CaCl2 followed by MF, and then subjected to CS, the seedlings growth were better than that of SNP followed by CS, and CaCl2 followed by CS. These results suggested that magnetic field compensates for the toxicological effects of cadmium exposure
are related to NO signal. 相似文献
322.
HPLC-ELSD法测定黄花蒿提取物中青蒿素 总被引:3,自引:0,他引:3
目的建立HPLC-ELSD法对黄花蒿提取物中青蒿素的测定方法。方法采用HPLC-ELSD法。色谱柱:Inerts il ODS-3(150 mm×4.6 mm,5μm);流动相为乙腈-水(69∶31,用三氟醋酸调pH至3);体积流量:1.0 mL/m in;检测器漂移管温度:45℃;载气体积流量:1.0 L/m in;分流:开;柱温:25℃。结果青蒿素在0.589 3~5.893 5m g/mL时面积的常用对数值与其质量浓度的常用对数值线性关系良好(r=0.989);样品平均回收率为98.71%(RSD为0.42%,n=6)。结论该方法灵敏、准确可靠,可作为黄花蒿提取物中青蒿素的定量方法。 相似文献
323.
《南京医科大学学报(英文版)》2007,21(1):25-28
ObjectiveTo measure the percent of free prostate specific antigen (fPSA) among men without prostate diseases in Xi' an area, and to study the relationship of percent fPSA with age and pathological grade, clinical stage of prostate cancer (PCa) with percent fPSA, and to analyze the difference between the data in China and the overseas data to determine appropriate reference range for Chinese male.MethodsA total of 713 participants were enrolled into the study, with PSA, fPSA in serum measured and the percent fPSA calculated. Out of 713 cases, 679 without prostate diseases were divided into 5 groups by age, and then the relationships of PSA, fPSA and percent fPSA with age were studied, respectively. The relationship of pathological grade and clinical stage with percent fPSA of the 34 participants with PCa was also studied. With the help of the related data of men without prostate disease, the appropriate reference range for Chinese male was established.ResultsThe increases in PSA or fPSA were correlated with age, while there was no significant correlation between age and percent fPSA. The percent fPSA was also correlated with pathological grade and clinical stage of PCa. The percent fPSA of men without prostate disease in Xi'an area was significantly lower than that in the related overseas data. The reference range of percent fPSA for Chinese male was ≥ 15%.ConclusionPercent fPSA might be more useful than PSA in the detection of prostate cancer. As the percent fPSA is decreased, the pathological grade is decreased, and the clinical stage is increased, the malignant degree is increased. The reference range of ≥ 15% is more appropriate for Chinese male. 相似文献
324.
325.
《Pathology, research and practice》2020,216(9):153121
Recently, a high-risk human papillomavirus (HR-HPV) detecting assay alone could be used as a first-line screening tool for cervical cancer, although the test system has been limited to the Cobas 4800 HPV test. However, the screening efficiency of the HPV chip, which is widely used in Eastern Asia because of the high prevalence of non16/18 HR-HPV genotypes, has not been well elucidated. After selecting 300 women who were co-tested using the PANArray HPV chip and the ThinPrep assay and had confirmed histological diagnoses, we evaluated the diagnostic accuracy of the PANArray HPV test based on direct sequencing and clinical performance compared to the ThinPrep alone and co-testing. HR-HPVs were identified in 212 (70.7 %) patients by the PANArray HPV test. The results of the PANArray HPV test and direct sequencing for detecting HR-HPVs were in almost perfect agreement, consistent in 95.3 % of the cases (k = 0.89). HR-HPVs were more commonly detected by the PANArray HPV assay in patients with high-grade squamous intraepithelial lesions (HSILs) or worse (p < 0.001, both) by cytological and histological examinations. The PANArray HPV test had higher sensitivity (91.7 %) than the ThinPrep (52.6 %) but co-testing increased the sensitivity for predicting HSIL or worse cervical lesions to 99.2 %. In conclusion, the PANArray HPV test accurately detected HR-HPVs determined by cytological and histological examinations to be HSIL or worse cervical lesions. The PANArray HPV assay alone was more sensitive than the ThinPrep alone for detecting HSIL or worse cervical lesions, however, co-testing enhanced the sensitivity. Co-testing is more useful for screening HSIL or worse lesions than use of either the ThinPrep or PANArray HPV genotyping alone. 相似文献
326.
通过对户县地区农民的调研和相关工作人员进行访谈,总结出户县地区新型农村合作医疗制度运行几年来存在的主要问题,并有针对性地提出完善制度的对策建议,也为其他地区新型农村合作医疗工作的开展提供有益的决策参考。 相似文献
327.
目的建立同时测定冠心舒通胶囊中10种成分含量的方法,构建心肌细胞缺氧/复氧(H/R)模型,并对测定的10种成分进行活性评价。方法采用HPLC法建立冠心舒通胶囊中10种成分的含量测定方法,并对其进行方法学考察以验证其可靠性。建立体外培养大鼠乳鼠心肌细胞H/R模型,测定各成分对H/R心肌损伤的作用。结果建立的HPLC法操作简便、快速、结果准确可靠。丹酚酸B、原儿茶酸、丹参素钠、丹参酮ⅡA、隐丹参酮、丁香酚和迷迭香酸对心肌细胞H/R损伤有明显的保护作用,而香草醛、没食子酸和原儿茶醛无保护作用。结论初步筛选出了冠心舒通胶囊治疗冠心病的7种活性成分,为后期进一步确定冠心舒通胶囊治疗冠心病的药效标志物提供依据。 相似文献
328.
筛选辅酶Q10自微乳化释药系统(CoQ10-SMEDDS)的处方并考察其稳定性。通过溶解度实验筛选油相、表面活性剂和助表面活性剂;绘制三元相图,以乳化时间、乳化效果和乳滴粒径大小为指标,确定最佳处方及配比;考察CoQ10-SMEDDS在各处理条件下的稳定性。CoQ10-SMEDDS最佳处方配比为油酸乙酯∶Cremo-phor EL∶PEG400为18∶35∶7,自乳化形成平均粒径为30.9 nm的微乳。所制备的CoQ10-SMEDDS对CoQ10的溶解度大,自乳化效果好,乳滴粒径小,稳定性高。 相似文献
329.
目的:制备胸腺五肽脂质体并进行质量评价。方法:采用复乳法制备胸腺五肽脂质体,以聚乳酸-羟基乙酸共聚物(PLGA)及卵磷脂为成球材料、以胸腺五肽为主药制备脂质体。以明胶浓度、PLGA浓度和卵磷脂浓度为考察因素,以包封率和载药量为考察指标设计L(934)正交试验优化基质处方并进行验证试验。通过测定优化处方所制脂质体粒径、包封率、体外累积释放百分率等评价脂质体质量。结果:优化基质处方为明胶、PLGA和卵磷脂浓度分别为100、200、100mg.mL-1。所制脂质体形态完整,平均粒径为(9.03±0.83)μm,载药量与包封率分别为(1.81±0.03)与(74.4±1.4),20d的累积释药百分率达90以上。结论:所制胸腺五肽脂质体工艺简单、重现性好,包封率和载药量高,具有显著的缓释作用。 相似文献
330.
目的 探究急性心肌梗死 (acute myocardial infarction, AMI) 并发阿斯综合征 (Adams-stokes syndrome) 患者血清和肽素与 Adropin 水平联合检测的早期诊断价值研究。方法 选取西安高新医院急诊科 2016 年 1 月 ~2020 年 1 月收治的 70 例 AMI 并发阿斯综合征患者为观察组,另随机选取同期 70 例健康体检者为对照组。应用酶联免疫吸附法 (ELISA)检测两组血清和肽素和 Adropin 的水平,绘制受试者 ROC(receiver operating characteristic) 工作曲线,评估血清和肽素,Adropin 和两者联合检测的诊断特性。结果 血清 Adropin ≥ 170.4 pg/ml 为 AMI 并发阿斯综合征的临界值。两组AMI 并发阿斯综合征发病后 0,2,4 和 6 h 时和肽素和血清中 Adropin 水平差异均具有统计学意义 (U=5.325~294.457,均 P < 0.05)。不同发病时间点和肽素联合 Adropin 的异常率均高于其单独检测。和肽素灵敏度为 91.02%,特异度为 74.51%。血清中 Adropin 灵敏度为 86.83%,特异度为 77.98%,将两者联合灵敏度提高至 92.71%,特异度提高至82.65%。结论 血清和肽素联合 Adropin 在 AMI 并发阿斯综合征发病初期具有较高的异常率,其灵敏度和特异度均高于两者单独检测,可用于 AMI 并发阿斯综合征的早期识别和诊断。 相似文献