胸膜斑阳性接尘工人血清效应蛋白的研究

目的 监测胸膜斑阳性接尘工人血清效应蛋白质,探讨胸膜斑发病机制及其与石棉肺的关系.方法 将研究对象分为胸膜斑组、阴性对照组和正常对照组.各组人群静脉血应用双向电泳和质谱进行分离和鉴定,以灰度值差异1.5倍为基准获得差异表达蛋白质.结果 胸膜斑组与阴性对照组和正常对照组比较,差异点分别有34和16个;阴性对照组与正常对照组比较,差异点有32个.效应蛋白质如补体因子、α1-抗胰蛋白酶等参与机体免疫、抗肿瘤等过程.部分效应蛋白质与石棉肺血清效应蛋白一致.结论 长期接触石棉导致血清中某些蛋白质表达量改变,进而影响其生物学功能.胸膜斑和石棉肺有相同的靶效应蛋白,提示胸膜斑工人更易患石棉肺.     

二圣救苦丹的物质基础及作用机制研究

目的：以系统药理学的方法探讨二圣救苦丹的药效物质基础和分子作用机制。方法：利用中药系统药理学数据库与分析平台（TCMSP）获取二圣救苦丹的化学成分,并根据Lipinski规则（LR）、口服生物利用度（Oral bioavailability,OB）以及药物相似性（Drug-likeness,DL）对潜在的生物活性成分进行分析鉴定;利用BATMAN-TCM对获得的活性成分进行生物信息学分析。结果：从二圣救苦丹中根据LR、OB及DL特性筛选出18种潜在有效成分。进一步筛选后获得了27条生物过程（BP,biologicalprocess）、11条细胞组分（CC,cellular component）、11条分子功能（MF,molecular function）。根据TTD数据库分析预测出二圣救苦丹潜在的治疗与流感,瘟疫相关的疾病16个。结论：二圣救苦丹可能是通过其活性成分群：大黄酸（Rhein）、谷固醇（beta-Sitosterol）、芦荟大黄素（Aloe emodin）、（-）-儿茶素[（-）-catechin]、豆甾醇（Stigmasterol）、谷甾醇（Sitosterol）等,影响FPGS、TYMS、IL13、PPARG、PTGS1、PTGS2、ANPEP、CHRM1、CHRM2、CHRM4、ANPEP、CNR1等靶点,调控糖酵解和糖质新生、类固醇生物合成、氧化磷酸化、花生四烯酸代谢、ABC转运蛋白、PPAR信号通路、心脏肌肉收缩、脂肪的消化和吸收、胆汁分泌等通路,从而发挥抗疟疾、自身免疫性疾病、严重急性呼吸系统综合征、冠状病毒感染、偏头痛、支气管痉挛等功能,达到抗瘟疫的目的。     

三黄漱口液治疗青春期龈炎的临床效果观察

目的观察三黄漱口液治疗青春期龈炎的临床效果。方法将44例青春期龈炎患者随机分成两组,每组各22例。医生首先对受试者进行基线检查,试验组以三黄漱口液漱口,对照组以生理盐水漱口,疗程均为1月。观察临床疗效及治疗前、治疗后牙龈指数、菌斑指数的变化。结果治疗1月后,试验组口腔内牙龈指数、菌斑指数明显低于对照组,两组比较,有显著性差异(P<0.05)。试验组总有效率95.44%,对照组总有效率63.63%,有显著性差异(P<0.05)。结论三黄漱口液对青春期龈炎、牙龈红肿以及抑制菌斑生长有明显效果。     

Protection studies following bronchopulmonary and intramuscular immunisation with yersinia pestis F1 and V subunit vaccines coencapsulated in biodegradable microspheres: a comparison of efficacy

We have compared the ability of intramuscularly and intratracheally administered recombinant F1 and V subunit antigens to safeguard mice from a lethal systemic challenge with plague. The combined subunits (1 microg V plus 5 microg F1) were inoculated either in the 'free' state as a solution, or entrapped within microspheres composed of a biodegradable polyester (Poly-L-lactide), on day 1 and 60 of the experiment. In comparison to the other regimens, introduction of microsphere suspensions into the respiratory tract resulted in statistically elevated levels of specific immunoglobulins in day 82 lung wash samples. A subcutaneous challenge with virulent Yersinia pestis bacteria on day 137, equivalent to more than 10(5) mouse LD(50)s, was comparatively well tolerated by all subunit treatment groups (with survival rates between 66 and 90%). In contrast, 80% of the mice injected intramuscularly with soluble F1 and V were defeated by a 10(7) MLD(50) subcutaneous challenge, whereas the group immunised intramuscularly with microparticles were significantly better protected (p<0.1) with 50% survival. Similarly, mice immunised intratracheally with microparticles were significantly better safeguarded (56% survival) compared with the group immunised with soluble subunits intramuscularly (p<0.01). Soluble sub-units delivered intratracheally afforded 33% protection against 10(7) MLD(50)s. These data indicate that bronchopulmonary administration of microsphere co-encapsulated recombinant F1 and V antigens elicits a similar level of protective immunity against systemic plague infection as that evoked by injecting co-encapsulated subunits into the muscle. Such findings corroborate the thesis that introduction of appropriately formulated F1 and V subunits into the respiratory tract may be an alternative to parenteral immunisation schedules for protecting individuals from plague.     

Passive antibody administration (immediate immunity) as a specific defense against biological weapons

The potential threat of biological warfare with a specific agent is proportional to the susceptibility of the population to that agent. Preventing disease after exposure to a biological agent is partially a function of the immunity of the exposed individual. The only available countermeasure that can provide immediate immunity against a biological agent is passive antibody. Unlike vaccines, which require time to induce protective immunity and depend on the host's ability to mount an immune response, passive antibody can theoretically confer protection regardless of the immune status of the host. Passive antibody therapy has substantial advantages over antimicrobial agents and other measures for postexposure prophylaxis, including low toxicity and high specific activity. Specific antibodies are active against the major agents of bioterrorism, including anthrax, smallpox, botulinum toxin, tularemia, and plague. This article proposes a biological defense initiative based on developing, producing, and stockpiling specific antibody reagents that can be used to protect the population against biological warfare threats.     

广东省2001～2005年动物鼠疫监测分析

目的分析广东省2001—2005年动物鼠疫监测结果，评估省内可能发生动物鼠疫的危险因素。方法对广东省2001-2005年鼠疫疫源地内、外的动物鼠疫监测资料整理统计并进行分析。结果家栖宿主动物平均总密度6．46％，其中黄胸鼠平均密度3．49％，黄胸鼠是优势种，占总捕获动物的54．42％；野栖宿主动物平均总密度6．30％，黄毛鼠是优势种，占总捕获动物的57，09％；家栖黄胸鼠鼠体蚤平均指数2．26，印鼠客蚤平均指数2，21；野栖鼠鼠体蚤平均指数0．98，伍氏病蚤是优势种，占鼠体蚤的82．37％；病原学和血清学监测均未发现有阳性检材。结论广东省未发现有鼠疫流行的信息，但宿主年口媒介动态存在着不容乐观的因素。     

简析清代《鼠疫汇编》的理法方药

通过对《鼠疫汇编》、《恶核良方释疑》、《鼠疫约编》、《鼠疫抉微》四部鼠疫专书研究，认为四书相辅相承，完整概述了清代中医对鼠疫证理法方药的研究，并从病因病机，诊断，临床表现、治法，预防调摄等方面，介绍清代鼠疫研究概况。     

2007年清新县鼠疫监测情况分析

目的 分析清远市鼠疫疫情、人群及媒介宿主动物监测情况,为制定鼠疫防治措施提供科学依据.方法 在全市范围开展鼠疫疫情监测,在清新县设立人间和宿主动物间监测点,采集健康人血,捕捉活鼠进行血清学和病原学检验.对鼠密度和传播媒介鼠体蚤指数进行统计分析.结果 2007年监测期间无鼠疫疫情和自毙鼠疫情报告;采集206份健康人血清进行鼠疫间接血球凝集试验(IHA-F1抗体)全部阴性;共捕获8种鼠形动物(活体和死体)381只,其中褐家鼠占70.52%,黄胸鼠占11.28%.板齿鼠占8.66%,黄毛鼠占3.41%.施氏屋顶鼠占3.15%:臭鼩鼱占1.57%,小家鼠占1.05%,青毛鼠占0.26%;家栖鼠占84.51%,野栖鼠占15.49%;雌雄鼠性别比例为1.31∶1;总鼠密度为10.76%,家栖鼠密度为10.14%,野柄鼠密度为11.33%;家栖鼠鼠体蚤指数为0.21,野栖鼠鼠体蚤指数为0.16 .鼠体蚤总指数为0.2,印鼠客蚤指数为0.15,游离蚤总指数为0;采集活鼠血清318份进行鼠疫IHA-F1抗体检测全部阴性;对病变鼠脏器172份压印培养鼠疫杆菌,结果全部阴性.结论 清新县主要鼠种是褐家鼠,主要蚤种是印鼠客蚤,暂未发现鼠类和人群的鼠疫感染情况.但仍存在鼠疫疫源地生态环境所具有的条件,应扩大监测范围,加强鼠疫相关宿主动物和传播媒介的监测工作.