HA/ZrO2 不同复合材料对MSCs贴壁、增殖及成骨分化影响的差异

目的: 观察间充质干细胞(MSCs)在不同生物材料表面的生长及成骨分化情况。方法: 采用干铺法制备氧化锆(ZrO₂)单层复合羟基磷灰石(HA)及ZrO₂梯度复合HA 两种复合材料,观察复合材料表面形貌特征。分离和培养兔MSCs,分别培养于HA/ZrO₂单层复合材料、HA/ZrO₂梯度复合材料、纯HA及ZrO₂材料薄片上,观察细胞贴壁、增殖情况和碱性磷酸酶活性,提取细胞总RNA并检测Ⅰ型胶原、骨钙蛋白和骨桥蛋白mRNA 表达情况。结果: 制备的HA/ZrO₂单层复合材料具有不连续的HA表面层,可以清晰地观察到部分ZrO₂基体。而HA/ZrO₂梯度复合材料表面较为粗糙,呈多孔状。X射线衍射分析显示,经高温烧结后,两种复合材料表面的ZrO₂相依旧存在,HA相转变为β-Ca₃(PO₄)₂(β-TCP)、α-Ca₃(PO₄)₂(α-TCP)和CaZrO₃相。细胞培养显示,HA/ZrO₂梯度复合材料更有利于细胞贴壁。细胞在纯HA上碱性磷酸酶活性较其它组显著升高;细胞在复合材料和纯HA上Ⅰ型胶原、骨钙蛋白和骨桥蛋白表达较对照组均有不同程度升高,其中Ⅰ型胶原表达升高更为明显。结论: HA/ZrO₂梯度复合材料可促进MSCs的增殖,并可促进MSCs的成骨分化。     

羟基磷灰石/二氧化锆复合材料颗粒对骨髓间充质干细胞增殖及成骨分化的影响

Objective To evaluate the effect of the HA/ZrO2 composite particle on proliferation and osteogenesis of rabbit mesenchymal stem cells (MSCs) by using molecular biology methods.Methods The HA/ZrO2 composite particles were sintered at high temperature using the powder of HA and ZrO2 with different proportions.MSCs were isolated from rabbits and cultured.The effect of the composite particles on promoting cell proliferation of rabbit MSCs was detected using MTT method.Alkaline phosphatase activities were measured with ALP method.RT-PCR method was applied to measure the expression of collagen Ⅰ , osteocalcin and osteopontin mRNA.Results Pure HA pauicles and composite particles which contain HA promoted MSCs proliferation.Vonkossa staining showed that both pure HA particks and composite particles decreased the degree of positive stain of MSCs.ALP test showed that cell activity in the culture medium that contained pure HA particles or composite particles was significantly higher than that in the conventional culture medium and that contained pure ZrO2 particles (P ＜ 0.05＝.RT-PCR results showed that composite particles promoted the expression of collagen Ⅰ and osteocalcin gene.Conclusion The HA/ZrO2 composite particles promote proliferation and osteogenesis of MSCs.     

屈螺酮炔雌醇片联合地塞米松治疗多囊卵巢综合征临床效果分析

目的 观察多囊卵巢综合征(PCOS)患者采用优思明联合地塞米松联合治疗的临床效果。方法 选择我院收治的PCOS患者90例，随机分为单一组和联合组每组各45例。单一组采用屈螺酮炔雌醇片(优思明)治疗，联合组采用优思明+地塞米松治疗，两组均治疗3个月经周期。对比两组疗效、治疗前后实验室指标[卵泡生成激素(FSH)、黄体生成激素(LH)、血清抗苗勒管激素(AMH)、空腹血糖(FPG)、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)]、排卵情况(成熟卵泡数量、最大卵泡直径)、药物不良反应发生情况。结果 联合组疗效优于单一组，FSH水平高于单一组，LH、AMH水平低于单一组，成熟卵泡数量、最大卵泡直径大于单一组，差异有统计学意义(P<0.05);两组治疗后FPG、FINS、HOMA-IR、治疗期间药物不良反应总发生率比较，差异无统计学意义(P>0.05)。结论 应用优思明与地塞米松联合治疗PCOS患者，可提升临床治疗效果，对改善患者性激素异常、胰岛素抵抗有积极意义，可有效促进患者恢复正常排卵，且安全性高。     

参麦注射液联合美托洛尔缓释片对慢性充血性心力衰竭患者的疗效观察

慢性心力衰竭（chronic heart failure，CHF）是大多数心血管疾病的终末阶段，也是最主要的死亡原因之一。临床研究证明，CHF患者经美托洛尔长期治疗可显著改善症状，降低病死率和住院率。然而临床上由于患者乏力、胸闷等症状在美托洛尔治疗时常用量不足或剂量难以上提。本文拟对住院的慢性充血性心力衰竭患者在应用利尿剂、ACEI或ARB、强心剂、美托洛尔等药物治