DNT细胞分离鉴定及其在正常人外周血中含量的检测

目的 建立人DNT细胞的免疫磁性分离(megnetic activated cell sorting,MACS)方法,并检测其在正常人外周血中含量.方法 运用MACS法分离止常人外周血中DNT细胞,用台盼蓝染色流式细胞法检测选出细胞的活性和纯度,运用流式细胞法检测其在正常人外周血中含量.结果 MACS分选法分选出的DNT细胞活性＞97%,纯度为82.77%;DNT细胞占TCRαβ+T细胞的比例(6.25±2.61)%(n=45).结论 MACS法可简单迅速分选出高纯度DNT细胞,且不影响细胞活力.DNT细胞占TCRCD3+T细胞的比例为(6.25±2.61)%.     

不同敷料组合对烧伤患者供皮区创面微环境的影响

Objective To investigate the effect of dressing materials in various combinations on bum wound microenvironment and healing condition. Methods Two hundred donor sites with wounds of 0.3 mm in depth in 186 burn patients, who needed skingrafting and admitted to our ward were enrolled in study, and they were divided into A ( with dressing composed of aiginate + cotton pad for donor area), B ( with dressing composed of vaseline gauze + cotton pad for donor area) , C (with dressing composed of algi-nate+ foam dressing for donor area), D ( with dressing composed of vaseline gauze + foam dressing for donor area) groups according to random table method. Effect of dressings on wound evaporation and pH value were observed. Bacterial colonization, degree of pain complained by patients after dressing change, and wound healing time in each group were compared. Results One hundred and eighty-four patients complied with the study, while 2 patients were excluded due to untimely falling-off of the dressing. Wound evaporaton in respectively, among them B group showed optimal effect of keeping humidity (P<0.01). Wound pH value in A, B, C, D groups was 7.22±0.06, 7.41±0.03, 7.05±0.03, 7.34±0.06, respectively, among them it was highest in B group. The positive rate of bacteria in D group was highest (22.4%), and lowest in C group (4.0%). Pain was lightest in C group (score was 0.98±0.12), and most serious in B group ( score was 8.14±0.82). The shortest wound healing time was seen in C group (6.7±0.8 d) , and longest in D group (15.6±3.5 d). Conclusions Application of various dressings on similar wounds can pro-duce different wound microenvironment, which is closely related to wound healing time. Compared with pH value, humidity is the more important factor for wound healing.     

The relationship between CD4-CD8-T cells in the peripheral blood of patients with pancreatic carcinoma and IL-4,IFN-γ levels

Objective To investigate the relationship between CD4-CD8-T cells ratio and IL-4, IFN-γ levels in the peripheral blood of patients with pancreatic carcinoma. Methods Floweytometer was used to analyze the CD4-CD8-T cells ratio in the peripheral blood of patients with pancreatic carcinoma and the IL-4 ,IFN-γ levels were detected by ELISA. Results The ratio of CD4-CD8-T cell in CD3+ T cell from 25 pancreatic carcinoma patients was(4.2 ± 1.7) %, the ratio of CD4-CD8-T cell in CD3+T cell from 45 healthy person was(6.3 ± 2.6) %, there was significant deviation between the two groups(P ＜ 0. 01). The IL-4 level of 25 pancreatic carcinoma patients was (86.3 ± 23.3) fg/L,the IL-4 level of 45 healthy person was (56.2 ± 9.2) fg/L,there was significant deviation between the two groups(P ＜0.01). The IFN-γ level of 25 pancreatic carcinoma patients was (16.4±4.8) fg/L before operation, the IFN-γ level of 45 healthy person was (27.4±3.8) fg/L,there was significant deviation between the two groups(P ＜0.01). The ratio of CD4-CD8-T cell in pancreatic carcinoma patient after operation was higher than before operation. It could be found negative correlation between CD4-CD8-T cells ratio and IL-4 level in pancreatic carcinoma patient,it could also be found positive correlation between CD4-CD8-T cells ratio and IFN-γ level in pancreatic carcinoma patient. In pancreatic carcinoma patient,the CD4-CD8-T cells ratio and IL-4 level was significant associated with clinical stage (P ＜ 0.05), but no relationship with hisological differentiation (P＞0.05), it could be found no relationship between IFN-γ level and clinical stage, hisoiogieal differentiation (P ＞ 0.05). Conclusion The CD4±CD8±T cells ratio in the peripheral blood of patients is decreased,it may be participate in the carcinogenesis and development of pancreatic carcinoma by influence the IFN-γ levels.     

新生儿缺氧缺血性脑病36例多层螺旋CT的诊断价值

<正>新生儿缺氧缺血性脑病(HIE)是由于各种围生期窒息引起的脑组织缺氧、脑血流减少或暂停而导致胎儿损伤,是引起新生儿急性死亡和慢性神经系统损伤的主要原因之一,本病只根据临床表现很难确诊,而CT却能提供准确诊断,对临床正确处理窒息儿及推测其预后有一定意义。     

胰管结石的诊断与治疗

胰管结石是慢性胰腺炎特殊表现,胰管阻塞是引起腹痛、消化道紊乱等症状的主要原因[4].胰管结石是-种比较少见的疾病,国内这方面报道甚少.我院从1982年1月至2000年5月共收治胰管结石14例.现就胰管结石的诊断及治疗进行探讨. 临床资料 1.一般资料:男3例,女11例.年龄18～50岁.病人无嗜酒史、无营养不良史.临床上主要表现间歇性上腹部疼痛伴腰背部放射,其中继发性糖尿病6例、脂肪泻2例、血尿淀粉酶升高5例、黄疸3例、胰头癌1例.     

致伤物嵌入颅内致开放性颅脑损伤四例

致伤物嵌入颅内是一种特殊的开放性颅脑损伤，临床上相对少见，在诊治上具有特殊性。我院1999年1月-2006年6月共收治4例，效果满意。报告如下。     

不同敷料组合对烧伤患者供皮区创面微环境的影响

Objective To investigate the effect of dressing materials in various combinations on bum wound microenvironment and healing condition. Methods Two hundred donor sites with wounds of 0.3 mm in depth in 186 burn patients, who needed skingrafting and admitted to our ward were enrolled in study, and they were divided into A ( with dressing composed of aiginate + cotton pad for donor area), B ( with dressing composed of vaseline gauze + cotton pad for donor area) , C (with dressing composed of algi-nate+ foam dressing for donor area), D ( with dressing composed of vaseline gauze + foam dressing for donor area) groups according to random table method. Effect of dressings on wound evaporation and pH value were observed. Bacterial colonization, degree of pain complained by patients after dressing change, and wound healing time in each group were compared. Results One hundred and eighty-four patients complied with the study, while 2 patients were excluded due to untimely falling-off of the dressing. Wound evaporaton in respectively, among them B group showed optimal effect of keeping humidity (P<0.01). Wound pH value in A, B, C, D groups was 7.22±0.06, 7.41±0.03, 7.05±0.03, 7.34±0.06, respectively, among them it was highest in B group. The positive rate of bacteria in D group was highest (22.4%), and lowest in C group (4.0%). Pain was lightest in C group (score was 0.98±0.12), and most serious in B group ( score was 8.14±0.82). The shortest wound healing time was seen in C group (6.7±0.8 d) , and longest in D group (15.6±3.5 d). Conclusions Application of various dressings on similar wounds can pro-duce different wound microenvironment, which is closely related to wound healing time. Compared with pH value, humidity is the more important factor for wound healing.     

茶树油杀菌效果分析

目的 验证茶树油对致病微生物的杀灭作用,寻找有效、方便实用的新一代消毒剂.方法 用军科院提供的金黄色葡萄球菌ATCC6538(第4代)、白色链珠菌ATCC10231(第9代)、大肠埃希菌8099(第4代)用重庆产茶树油作三次重复定量杀菌试验.结果 茶树油对以上三种微生物10分钟杀灭作用达分别为92.46%、76.49%、99.99%.结论 茶树油可作为制造有效、方便、实用的消毒剂之原料.     

VEGF-C和VEGF-D在胰腺癌中表达及其意义

目的 探讨VEGF-C和VEGF-D在胰腺癌中表达及其与胰腺癌临床病理学特征之间的关系.方法 运用免疫组织化学(SP法)检测30例胰腺癌、癌旁组织、正常胰腺组织中VEGF-C、VEGF-D蛋白表达,且比较VEGF-C、VEGF-D蛋白表达与临床病理等因素之间的相关性.结果 VEGF-C、VEGF-D蛋白在胰腺癌中表达分别为70%(21/30)、73%(22/30)且在胰腺癌、癌旁组织、正常组织表达呈下降趋势.VEGF-C、VEGF-D表达与淋巴转移、临床病理分期呈正相关性(P＜0.05),与胰腺癌组织学分化呈负相关(P＜0.001).结论 VEGF-C、VEGF-D与胰腺癌发生、发展密切相关,与胰腺癌的临床分期、组织分化、以及淋巴转移密切相关,VEGF-C、VEGF-D有望成为胰腺癌抗淋巴道转移的治疗的靶蛋白.     

奥曲肽抑制转化生长因子α诱导肝癌细胞增殖的实验研究

目的探讨奥曲肽对转化生长因子α（TGF-α）诱导肝癌细胞增殖的抑制作用及其机制。方法用免疫组化法观察奥曲肽对TGF-α诱导肝癌细胞增殖的影响，用逆转录-聚合酶链反应（RT-PCR）检测奥曲肽对肝癌细胞TGF-α分泌和表皮生长因子受体（EGFR）表达的影响，用Western-blot法、免疫组化法检测奥曲肽对细胞外信号调节蛋白激酶（ERK）的影响。结果奥曲肽明显抑制细胞核内TGF-α诱导的增殖细胞核抗原（PCNA）表达，使肝癌细胞TGF-αmRNA指数较对照组降低（P〈0．05），对TGF-α诱导的EGFR mRNA指数的增加具有明显抑制作用。Western-blot显示，奥曲肽明显抑制TGF-α诱导的ERK蛋白表达；免疫组化显示，TGF-α作用后，胞核染色明显加深，奥曲肽与TGF-α同时作用后胞核染色明显减弱。结论奥曲肽可能通过抑制肝癌细胞分泌TGF-α，抑制EGFR表达和阻断EGFR信号传导，从而抑制TGF-α诱导的肝癌细胞增殖。