Effect of Powdered Fermented Milk with Lactobacillus helveticus on Subjects with High-Normal Blood Pressure or Mild Hypertension

Objective: Two tripeptides (Val-Pro-Pro and Ile-Pro-Pro) that have inhibitory activities for angiotensin I-converting enzyme are produced in milk fermented with Lactobacillus helveticus. In this study we evaluated the effect and safety of powdered fermented milk with L. helveticus CM4 on subjects with high-normal blood pressure or mild hypertension.

Methods: A randomized, placebo-controlled, double-blind study was conducted using 40 subjects with high-normal blood pressure (HN group) and 40 subjects with mild hypertension (MH group). Each subject ingested 6 test tablets (12 g) containing powdered fermented milk with L. helveticus CM4 daily for 4 weeks (test group) or the same amount of placebo tablets for 4 weeks (placebo group).

Results: During treatment, the decrease in systolic blood pressure (SBP) and diastolic blood pressure (DBP) in the test group tended to be greater than in the placebo group for both blood pressure groups. At the end of treatment (week 4), a significant decrease in DBP in the HN group was observed (i.e. 5.0 mm Hg (0.1, 9.9; p = 0.04) compared with the placebo group). There was no significant change in SBP (3.2 mm Hg (95% CI ?2.6, 8.9; p = 0.27). In the MH group, SBP decreased by 11.2 mm Hg (4.0, 18.4; p = 0.003) and there was a statistically non-significant decrease in DBP of 6.5 mm Hg (?0.1, 13.0; p = 0.055) compared with the placebo group. No marked changes were observed in other indexes, including pulse rate, body weight and blood serum variables, and no adverse effects attributed to the treatment was found in each group.

Conclusions: Daily ingestion of the tablets containing powdered fermented milk with L. helveticus CM4 in subjects with high-normal blood pressure or mild hypertension reduces elevated blood pressure without any adverse effects.     

瑞士乳杆菌发酵乳对去卵巢大鼠骨密度和骨强度的影响

<正>瑞士乳杆菌发酵乳较普通酸乳能更好提高血清钙的含量[1],本研究探讨长期食用瑞士乳杆菌发酵乳对去卵巢大鼠的骨密度、骨矿物质及骨强度影响。     

婴儿肝炎综合征应用微生态调节剂后肠道菌群及胆汁成分的随机双盲对照临床研究

摘要：目的　探讨微生态调节剂乐托儿（冻干灭活的嗜酸乳杆菌）对婴儿肝炎综合征（infantile hepatitis syndrome,IHS）肠道菌群及胆汁成分的影响。方法　2002年3月至2008年5月在华中科技大学同济医学院附属同济医院儿科住院的60例IHS为纳入研究对象,随机分为乐托尔组和对照组,随机方法为随机数字表法。患儿均给予熊去氧胆酸（25 mg/次,日3次）及护肝治疗。乐托尔组口服乐托尔散剂（嗜酸乳杆菌散剂）每次1袋,2次/d;15 d为1个疗程,连续2个疗程。对照组口服中和冻干培养基。监测两组患儿治疗前后血清和十二指肠引流液（胆汁）中总胆红素（TB）、结合胆红素（DB）、丙氨酸转氨酶（ALT）、γ-谷氨酰转肽酶（γ-GT）、总胆汁酸（TBA）水平及肠道菌群数量。采用SPSS 10.0软件进行统计学分析。结果　两个疗程结束后,乐托尔组总有效率为92.86%,对照组为74.07%,差异有统计学意义（P < 0.05）。乐托尔组患儿血清中TB、DB、ALT、γ-GT、TBA与对照组比较均显著降低,差异有统计学意义（P < 0.05）。乐托尔组肝脏明显缩小,差异有统计学意义（P < 0.05）。十二指肠引流液中TB、DB、γ-GT、TBA与对照组比较均显著升高,差异有统计学意义（P < 0.05）。对照组肠道菌群无明显变化,乐托尔组双歧杆菌、乳酸杆菌、粪肠球菌显著增加,差异有统计学意义（P < 0.05）。两组均未见明显药物副反应。结论　IHS存在肠道菌群紊乱,微生态调节剂（冻干灭活的嗜酸乳杆菌）与熊去氧胆酸合用能促进胆汁酸排泄,改善肝功能及肠道菌群,在IHS的治疗中有明确的有效性。     

Microencapsulation of probiotics by efficient vibration technology

The target site of action of probiotics is the intestine. They must be surviving the stomach acidic condition before reaching the target site. Three probiotic bacteria were microencapsulated in sodium alginate beads using a sophisticated microencapsulation technology provided by BÜCHI B-390. This study reports the tolerance of the different microencapsulated Lactobacillus at low pH using simulated gastric juice, comparing it with the tolerance of free bacteria. The three microencapsulated strains displayed time-dependent acid sensitivity at pH values under 3.0. At pH 2.0, a dramatic reduction in bacterial survival occurred after 5?min, with only L. casei surviving after 30?min, with 75% survival. At pH 2.5 microencapsulated L. casei survived for 90?, L. reuteri survived for 60?and L. bulgaricus survived for only 30?min, respectively. The microencapsulation technology used in this study may effectively protect Lactobacillus from gastric conditions and permit comparisons between strains.     

门诊腹泻病患儿的初筛处理方案及疗效探讨

目的通过病例对比论证使用益生菌制剂作为初筛手段来治疗门诊儿童轻度腹泻病的可行性,探索出一个快捷、简便、有效的初筛用药方案,以提高门诊的工作效率。方法从常用益生菌制剂中选出复方嗜酸乳杆菌片及妈咪爱作为研究对象。将2010年6月～2012年6月于我院门诊就诊,诊断为轻度腹泻病的患儿共120例随机分成两组,治疗组60例给予复方嗜酸乳杆菌片口服,对照组60例给予妈咪爱口服,两组均配合蒙脱石散治疗。结果两组治疗有效率均超过50%,分别为78.3%、55.0%,两组有效率比较差异存在显著性(p<0.01)。两组用药治疗期间均未发生不良反应。结论使用复方嗜酸乳杆菌片或妈咪爱益生菌制剂来治疗儿科门诊轻度腹泻病患者具有可行性,可作为初筛治疗方案应用,且复方嗜酸乳杆菌片效果优于妈咪爱。     

Lactobacillus helveticus–fermented milk improves learning and memory in mice

Abstract

Objectives

To investigate the effects of Calpis sour milk whey, a Lactobacillus helveticus–fermented milk product, on learning and memory.

Methods

We evaluated improvement in scopolamine-induced memory impairment using the spontaneous alternation behaviour test, a measure of short-term memory. We also evaluated learning and working memory in mice using the novel object recognition test, which does not involve primary reinforcement (food or electric shocks). A total of 195 male ddY mice were used in the spontaneous alternation behaviour test and 60 in the novel object recognition test.

Results

Forced orally administered Calpis sour milk whey powder (200 and 2000 mg/kg) significantly improved scopolamine-induced cognitive impairments (P < 0.05 and P < 0.01, respectively) and object recognition memory (2000 mg/kg; P < 0.05).

Discussion

These results suggest that Calpis sour milk whey may be useful for the prevention of neurodegenerative disorders, such as Alzheimer's disease, and enhancing learning and memory in healthy human subjects; however, human clinical studies are necessary.     

Some probiotic and antibacterial properties of Lactobacillus acidophilus cultured from dahi a native milk product

In this study, different strains of Lactobacillus acidophilus from dahi were analyzed for certain probiotic and antibacterial properties. Initially, these strains were confirmed by the amplification of 16S rRNA regions and then screened for antibacterial activities against food borne pathogens. The phenotypic relationship between apparent antibacterial activity and cell wall proteins were established by cluster analysis. It was observed that those strains, which have prominent bands having size 22–25?kDa possess antibacterial activity. On the basis of wide spectrum of killing pattern, a strain LA06FT was further characterized that showed no change in its behavior when subjected to the antibiotic protected environment and grow well in acid-bile conditions. The bacteriocin produced by this strain has specific antibacterial activity of 5369.13?AU?mg^?1. It remained stable at 60–90?°C and pH range of 4.5–6.5 while proteolytic enzymes inactivate the bacteriocin that confirm its proteinic nature having molecular weight of ≤8.5?kDa.     

治疗真菌性阴道炎的临床分析

目的探索真菌性阴道炎的有效的合理的治疗方案。方法把门诊确诊为真菌性阴道炎的患者150例，随机分成3组，第一组治疗方案：用氟康唑重复顿服2次＋达克宁外用治疗3天；第二组治疗方案：氟康唑重复顿服2次＋达克宁外用3天后予以阴道用乳酸杆菌10天；第三组治疗方案：在第二组治疗方案的同时，检查及治疗性伴侣。停药后1周和2月复查。结果（1）疗效比较：三组的临床有效率分别是82％、96％、98％，第三组、第二组与第一组比较，P〈0．05，有差异；第二组与第三组比较P〉0．05，无差异；（2）复发比较：三组的复发率分别是36．58％、14．58％、2．04％，第一组与第二组比较，P〈0．05，有差异；第二组与第三组比较，P〈0．05，有差异；第一组与第三组比较，P〈0．05，有差异。结论（1）在真菌性阴道炎的治疗中，加用乳酸杆菌效果良好，值得推广。（2）在真菌性阴道炎的治疗中，对其性伴侣进行同时检查及治疗可以减少复发。     

乳酸菌对高脂血症小鼠血脂和肠道菌的影响

目的:研究功能乳酸菌制剂的降血脂作用及对肠道菌的影响。方法:建立小白鼠高脂血症动物模型;模型小鼠30只,随机分为:生理盐水组、复合乳酸菌组和辛伐他汀组,每组10只。采取灌胃处理,分别灌服生理盐水、复合乳酸菌制剂(等比例混合的植物乳杆菌、嗜酸乳杆菌和干酪乳杆菌)和降血脂药物辛伐他汀。结果:与生理盐水比较,乳酸菌和辛伐他汀药物处理显著降低了小鼠血清胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(HDL-C)含量(P0.05);乳酸菌处理和药物处理间TC、TG和HDL-C无显著差异。与降血脂药物相比,乳酸菌处理显著增加小鼠粪便乳酸菌数量(P0.05),降低了大肠杆菌(P0.05)和肠球菌数量(P0.01)。结论:乳酸菌制剂具有降低血脂、增加肠道有益菌数量、降低肠道有害菌数量的作用。