合并糖尿病的急性冠脉综合征患者共刺激分子与炎症指标的研究

目的探讨CD4^＋CD28^nullT淋巴细胞及炎症因子C反应蛋白（CRP）在合并糖尿病的冠心病患者发病机制中的作用。方法51例经冠状动脉造影确认为急性冠脉综合征（ACS）的患者,根据1999年WHO标准分为2型糖尿病合并ACS组21例,单纯ACS组30例,对所有研究对象均通过流式细胞术测量外周血中CD4^＋CD28^nullT淋巴细胞的数量,对比分析两组患者的临床资料和冠状动脉造影结果。结果与单纯ACS患者相比较,合并糖尿病的ACS患者外周血CD4^＋CD28^nullT淋巴细胞的数量显著增多[（4．66±4．24）％VS（8．89±6．45）％,JP〈0．05],CRP浓度无明显统计学差异[（1．97±1．05）VS（2．22±1．14）mg／L,P〉0．05],冠状动脉造影显示完全闭塞、弥漫病变比例较高（7w11,P〈0．05;6YS12,P〈0．01）。结论糖尿病合并ACS患者冠状动脉病变累及范围广且程度重,共刺激分子可能与合并糖尿病的ACS患者的免疫调节异常有关。     

Hb Hubei [α114(GH2)Pro→His,HBA1: c.344C>A]: A Novel Hemoglobin Variant of the α1-Globin Chain

Abstract

We report here a novel α1-globin chain variant, Hb Hubei [α114(GH2)Pro→His, HBA1: c.344C>A], in a Chinese individual. The proband, a 28-year-old Chinese female, was discovered following routine Hb A_1c analysis using cation exchange high performance liquid chromatography (HPLC). Sanger sequencing revealed a novel missense mutation, HBA1: c.344C>A (CCC>CAC), in exon 2 of the α1-globin gene. The mutation caused a transition of proline to histidine at position α114(GH2) on the α1-globin chain. This new variant was named Hb Hubei after the geographic origin of the proband.     

湖北地区汉族人群维生素D受体基因起始密码单核苷酸多态性与前列腺癌的相关性分析

目的 :了解维生素D受体 (vitaminDreceptor,VDR)基因起始密码单核苷酸多态性与前列腺癌的关系 ,探讨前列腺癌发病的分子机制。　方法 :提取 80例前列腺癌患者及 96例健康男性对照者外周血标本中基因组DNA ,应用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)及DNA测序技术检测并分析了VDR基因起始密码单核苷酸多态性在两组中的分布。　结果 :前列腺癌病例和对照组FokⅠ等位基因频率分布均符合Hardy Weinberg定律 ,其FokⅠ多态位点FF、Ff、ff基因型分布频率分别为 32 .5 %、5 0 .0 %、17.5 %和 2 5 .0 %、5 3.13%、2 2 .87% ;等位基因F、f分布频率分别为 4 5 %、5 5 %和 5 1.5 6 %、4 8.4 4 % ,在湖北地区汉族前列腺癌患者及对照者中的分布频率差异无显著性 (P >0 .0 5 )。　结论 :VDR基因起始密码的单核苷酸多态性可能与低发病的湖北地区汉族人群的前列腺癌发生无关。     

神农架的中药资源

神农架林区有中药2023种,隶属于300科974属。其中植物药有1800种(含亚种),动物药有213种,矿物药有10种。本文还探讨了它们的保护、开发和利用。     

2009年十堰市健康人群流脑抗体水平监测结果分析

采用酶联免疫吸附试验(ELISA)对十堰市健康人群A群和C群流脑抗体水平进行检测。结果 A群流脑抗体阳性率平均为91.41%(383/419),几何平均滴度(GMT)为1∶11.94;C群流脑抗体阳性率仅为13.21%(56/424),GMT为1∶1.94。十堰市可能存在C群脑膜炎链球菌的自然感染。     

2010年湖北省血吸虫病疫情监测

目的掌握2010年湖北省血吸虫病监测点疫情。方法按照《湖北省血吸虫病监测方案》的要求,在全省血吸虫病流行区选择有代表性的207个流行村作为监测点,开展人畜查病和钉螺调查。结果 207个监测点居民血吸虫感染率为1.01%,耕牛感染率为1.14%,活螺平均密度为0.50只/0.1m2,感染螺平均密度为0.0004只/0.1m2,钉螺感染率为0.08%。结论与2009年相比,2010年湖北省血吸虫病疫情出现了明显下降,但疫情仍不稳定且容易反复,需进一步加大传染源控制力度,加强耕牛管理以及查灭螺工作。     

Efficacy of extended versus standard lymphadenectomy in pancreatoduodenectomy for pancreatic head adenocarcinoma. An update meta-analysis

BackgroundSurgical resection is the only possible cure for pancreatic cancer, it remains controversial whether extend lymphadenectomy in pancreatoduodenectomy (EPD) is better than standard lymphadenectomy in pancreatoduodenectomy (SPD). The aim of this study was to compare the efficacy of EPD with SPD for pancreatic head adenocarcinoma.MethodsA specific search of online databases including PubMed, Web of Science, Embase, and Cochrane library was conducted from January 1990 to October 2018. Relative perioperative outcomes were synthesized. Single-arm meta-analysis was also performed.ResultsA total of eight studies involving 687 (342 vs 345) patients were included for analysis in our study. The number of lymph nodes harvested [24.54 vs 13.29; weighted mean difference (WMD) ?10.69, P = 0.000], operative time (469.84 min vs 354.85 min; WMD -99.09, P = 0.000), and diarrhea (postoperative three months) [45.1% vs 18.2%; odds radio (OR) 0.20, P = 0.014] were significantly higher in patients who underwent EPD than SPD. The perioperative complications (35% vs 28.8%; OR 0.79, P = 0.186), tumor size (3.27 cm vs 3.248 cm; WMD -0.11, P = 0.256), lymph node metastasis (66% vs 55.9%; OR 0.71, P = 0.105), and positive margin (10.4% vs 11.3%; OR 1.28, P = 0.392) were no significant differences between EPD group and SPD group. Extended lymphadenectomy in pancreatoduodenectomy dose not contribute to the overall survival of patients with adenocarcinoma of the pancreatic head [hazard ratio (HR) 0.95; 95% CI 0.78–1.15; P = 0.61].ConclusionThe update meta-analysis shows that EPD failed to improve the overall survival, may even lead to increased morbidity.     

11种厚朴中酚性成分的比较研究

目的 用HPLC法对湖北神农架和恩施产厚朴中酚性成分进行含量测定,并与不同地区的商品厚朴进行含量比较.方法 HPLC法:色谱柱为Agilent HC-C18(4.6mm×250mm×5μm);以甲醇-水(78:22)为流动相;检测波长为294mm.结果 恩施干皮的含量19.103%＞恩施枝皮4.744%＞神农架干皮3....     

Bidirectional association between depressive symptoms and type 2 diabetes mellitus: The China Health and Retirement Longitudinal Study

ObjectiveTo prospectively examine the bidirectional relationship between depressive symptoms and type 2 diabetes mellitus (T2DM) among middle-aged and elderly Chinese.MethodsParticipants were enrolled in 2011–2012 (Wave 1) and followed up in 2013–2014 (Wave 2) and 2015–2016 (Wave 3) in the China Health and Retirement Longitudinal Study. Depressive symptoms were evaluated by the Chinese language version of 10-item Center for Epidemiological Studies Depression Scale (CESD-10) at three waves. T2DM was assessed by biochemical biomarkers at Wave 1 and reported physician-diagnosis at Wave 2 and 3. Cox proportional hazards regression was applied to calculate hazard ratio (HR) and 95% confidence intervals (CIs) for the bidirectional association.ResultsParticipants with baseline depressive symptoms were 1.33 times as likely to develop T2DM (HR, 1.33; 95% CI: 1.06, 1.66), compared to their counterparts after adjusting for demographic characteristics and T2DM risk factors. The risk of T2DM increased linearly with higher severity of depression as determined by a higher CESD-10 score (P for trend ? 0.001). In addition, baseline T2DM was associated with increased risk of incident depressive symptoms (1.15; 1.00, 1.31) and persistent depressive symptoms (1.35; 1.03, 1.77).ConclusionThere is a positive bidirectional association between depressive symptoms and T2DM in middle-aged and elderly Chinese.