巢式RT-PCR检测前列腺癌患者外周血中PSA mRNA和PSM mRNA的比较

目的　盲法比较研究巢式反转录聚合酶链反应 (RT PCR)检测前列腺癌 (PC)患者外周血中前列腺特异性膜抗原(PSM)mRNA和前列腺特异性抗原 (PSA)mRNA在PC诊断中的真实性和可靠性 .方法　根据PSM和PSA的cDNA序列设计合成引物 .应用巢式RT PCR方法分别检测 36例PC患者和 2 3例前列腺增生 (BPH)患者外周血中PSMmRNA和PSAmRNA .结果　PSAmRNA和PSMmRNA阳性表达的灵敏度 (Sen)分别为 :2 9.7% ,6 7.6 % (P <0 .0 1) .特异度 (Spe)分别为 :86 .4 % ,10 0 .0 % (P <0 .0 5 ) .准确度 (Ac)分别为 :5 0 .8% ,79.8% (P <0 .0 5 ) .卡柏值 (κ)分别为 :0 .13(差 )、0 .6 (一般 ) .结论　应用巢式RT PCR方法 ,患者外周血中PSMmR NA在PC的诊断、微转移、术后随访、人群筛选中的真实性和可靠性明显优于PSAmRNA .     

CLINICAL APPLICATION OF RENYING (ST 9)

ThepointRenying(ST9)wasfirstmen-ti9nedinChapter2ofMraculousPifor(LingShu).LocatedontheStomachMeridianofFootYangming,itisalsoknownasTianwuhuiandWuhuiinChinese.ItistheconnectingpointoftheStomachMeridianofFoot-YangmingandGallbladderMeridianofFoot-Shaoyang.Inan-cienttimes,whereitissitedwasoneoftheplacesforthegeneralpulse-takingofthewholebody.ThepulsefeltherewascalledRenyingPulse.Thuscomesthnameofthepoint.Theauthorofthearticlenoticedbychanceintheear-ly6o'sthatthepointhadanobviouseffectfo…     

Positive fraction of systematic biopsies predicts risk of relapse after radical prostatectomy

Objectives. To determine whether the positive fraction of systematic sextant biopsies contributes to the prediction of serologic relapse after radical prostatectomy.Methods. A retrospective review of patients who underwent transrectal ultrasound-guided systematic sextant biopsy and radical prostatectomy was performed. No patients received neoadjuvant or adjuvant therapy. The relationship between the positive fraction of systematic biopsies and risk of prostate-specific antigen recurrence was assessed with Kaplan-Meier and multivariate analyses.Results. Patients with three or fewer positive sextant biopsies were at a significantly lower risk of relapse after radical prostatectomy than patients with four or more positive biopsies. Tumor grade and systematic biopsy results were the most powerful predictors of serologic relapse.Conclusions. The positive fraction of systematic biopsies contributes to the prediction of risk of relapse after radical prostatectomy.     

结肠定位给药系统载体的设计、合成及降解性能的研究

目的设计与合成结肠定位给药系统新型载体,探讨其模拟结肠环境下降解特性与载体结构关系。方法用甲基丙烯酸羟乙酯(HEMA)、甲基丙烯酸甲酯(MMA)和甲基丙烯酸(MAA)为单体,对二乙烯基偶氮苯(DVAB)为偶联剂,偶氮二异丁腈(AIBN)为引发剂,合成了聚甲基丙烯酸羟乙酯-甲基丙烯酸甲酯-甲基丙烯酸［P(HEMA-MMA-MAA)］三元偶氮共聚物,用紫外、红外、核磁共振谱进行结构表征。测定该聚合物溶胀性能,建立体外厌氧菌条件下模拟结肠内容液环境模型。用GPC,DSC与SEM比较聚合物降解前后,分子量、结构与形态变化。结果载体材料溶胀指标Q随材料中HEMA和MAA含量增加而增加。该材料在模拟结肠内容液环境下,发生降解程度与材料中三组分相对比例直接相关。结论合理调节材料中HEMA,MMA和MAA三组分比例,可望成为结肠定位给药系统的载体。     

Embouchure dystonia—Portrait of a task‐specific cranial dystonia

Focal task‐specific dystonia (FTSD) is an unusual disorder of motor control, which typically affects the hand but may also involve the face, jaw, and tongue. We report 89 musicians with dystonia of the embouchure (ED), the muscles of the lower face, jaw, and tongue used to control the flow of air into the mouthpiece of a woodwind or brass instrument. Symptoms of ED began at an average age of 36, were typically painless and only rarely were preceded by trauma. Specific musical techniques commonly triggered dystonia, often in one instrumental register. Task‐specific embouchure tremor and lip‐pulling ED phenotypes were common among high‐register brass players (trumpet and French horn), whereas lip‐locking occurred exclusively in low‐register brass players (trombone and tuba). Jaw and tongue ED phenotypes occurred predominantly in woodwind players, and once present, frequently spread to speaking or eating. Six percent of all ED patients had coincident writer's cramp, suggesting a possible genetic predisposition to develop dystonia. We assessed two‐point sensory discrimination in the upper lip, lower lip, and hand in ED patients, normal musicians, and nonmusician age‐matched controls—there were no differences between groups. Once present, symptoms of ED did not remit and often disrupted careers and livelihoods. Better treatments are urgently needed for this unusual disorder of oral motor control. © 2009 Movement Disorder Society     

血浆TNF-α变化对大鼠肝移植术后排斥反应的诊断作用

目的：测定原位肝移植大鼠术后血浆肿瘤坏死因子α（TNF-α）的浓度变化。探讨其在原位肝移植急性排斥反应中的作用。方法：利用酶联免疫检测法测定血浆TNF-α浓度，同时观察移植肝和胸腺病理形态变化。结果：（1）在SD→Wistar未处理组，术后7d内血浆TNF-α浓度几乎呈线性增加，但是在SD→Wistar DST处理组和Wistar→Wistar组，TNF-α浓度仅有轻度增加，第7天时达到峰值，随后逐渐下降，（2）在SD→Wistar未处理组，术后第12天移植肝出现典型的排斥反应表现，即肝细胞坏死，门静脉炎性浸润，中央静脉内皮细胞炎症和胆管损伤，在SD→Wistar DST处理组和Wistar→Wistar组，仅有轻度的胆管上皮增生。（3）在急性排斥反应期间，SD→Wistar未处理组的胸腺皮质和髓质内的胸腺细胞呈稀疏状分布，在SD→Wistar DST处理组和Wistar→Wistar组，未观察到胸腺组织有明显的变化。结论：TNF-α在肝移植排斥反应中发挥了重要作用。术后连续监测其浓度变化有一定的预测和早期诊断作用。     

手性液晶的研究——Ⅷ.2,5-二苯基嘧啶类

合成了3个系列、12个文题化合物。测定了它们的相变温度(△T=50～78℃)、液晶相类型和电滞回线,证明其具铁电性。其中7个为新的铁电液晶。     

恶性肿瘤特异性生长因子(TSGF)检测的临床意义

目的：探讨恶性肿瘤特异性生长因子（TSGF）测定对恶性肿瘤的诊断及疗效监测的应用价值。方法：采用TSGF快速诊断试剂盒，对30例正常人，36例良性肿瘤患者，17例急性炎症及136例恶性肿瘤患者治疗前和部分患者治疗后血清TSGF含量进行测定、比较。结果：恶性肿瘤患者血清中TSGF含量明显增高，且治疗后含量和阳性率都明显下降，对恶性肿瘤的敏感性为82．35％、特异性为96．67％。结论：TSGF的测定有利于恶性肿瘤的筛查诊断、疗效观察。     

Full automation in allergy testing: measurement of specific IgE by the ENEA System

We evaluated the ENEA System, a fully automated instrument for the measurement of specific IgE antibodies. The instrument dispenses sera and reagents, incubates, washes, and reads and prints results automatically. The "core" of the instrument is the reactive unit called ACE (Allergy Chamber Enzymatic), which is a new solid phase to which the allergens are linked. The system uses calibration curves specific for the major allergen families, and data are supplied qualitatively (five classes) and quantitatively. We evaluated the analytic efficiency of the system and its correlation with the in vivo test (skin prick test (SPT)) results in 60 patients with inhalant allergic diseases and in 20 controls. Results: 450 results were available within 4 h. A satisfactory within-run (CVs between 1.58 and 6.2%) and between-run (CVs 6.3–11.5%) precision was found. No significant earn-over was observed. A wide linearity of the assay was demonstrated. With the concordance between the clinical history and SPT as the reference value, the clinical sensitivity of the ENEA System was 84.1 %, the specificity 82.8%, and the overall efficiency 83.4%. Finally, a good agreement with the results of another technique for the in vitro measurement of specific IgE (Pharmacia CAP System) was proven.