反相高效液相色谱法测定保健用品中的延胡索乙素

目的建立用反相高效液相色谱测定保健用品中延胡索乙素含量的方法。方法样品中的延胡索乙素经氨水一甲醇提取、甲醇稀释定容、C18反相柱分离，在紫外检测器280nm波长处检测。结果此方法的回收率为86．2％～93．2％，RSD为6．5％。结论此方法操作简单，准确可靠，为保健用品中延胡索乙素含量的检测提供参考。     

上海市某幼儿园1995-2005年儿童乳牙患龋状况分析

目的了解上海市幼儿患龋状况及变化趋势,为幼儿龋病预防和治疗工作提供科学依据。方法分别于1995年、2000年和2005年对复旦大学医学院附属幼儿园2～5岁儿童进行口腔健康检查,统计受检儿童的患龋率、受检者龋均(dft)及龋蚀指数(CSI)。结果受检儿童的患龋率从1995年的61.3%下降至2005年的46.5%(P<0.01),受检者龋均(dft)从1995年的(3.11±3.79)降至2005年的(2.12±3.18)(P<0.01),龋蚀指数(CSI)没有下降。每次检查儿童患龋率、受检者龋均(dft)及龋蚀指数(CSI)均呈现由低年龄组到高年龄组升高的趋势。结论该幼儿园儿童患龋状况有所好转,儿童龋病防治工作取得一定成效。但有一部分儿童患龋情况特别严重,有"两极化"的趋势。     

社区口腔健康教育初探

社区口腔健康教育是改善和提高社区人群口腔健康状况的基本途径。基于我国国民口腔健康知识缺乏和口腔疾病高发而口腔专业人员匮乏的状况,社区口腔健康教育有其发展的必要性。在其发展的过程中,我们应该做到充分利用人力资源,多途径有针对性的教育,同时将口腔健康教育纳入到整个健康教育之中。     

Menstruation: choosing whether...and when

For many women in the United States, menstruation is a major health concern because menstrual disorders and other conditions that may be aggravated during menses (e.g., migraine headaches, epilepsy) carry substantial morbidity. Women today menstruate nearly 3 times as often as in primitive societies, and evidence suggests that frequent, repetitive menstrual cycles may increase health risks. Because the conventional 21/7 combination oral contraceptive (OC) regimen provides only limited relief for women with menstrual disorders, alternative OC regimens that reduce menstrual frequency have been proposed. A new OC formulation specifically designed to decrease menstrual bleeding to 4 times per year is currently under investigation. Most women welcome less frequent menses or even amenorrhea. Women who may derive particular benefit from reduced menstrual frequency include not only those with medical conditions directly caused or aggravated by menses, but also those serving in the military, female athletes, mentally-retarded women with menstrual hygiene problems, young teens, and perimenopausal women.     

康赛德止痛液中陈皮、白术和党参的薄层鉴别

采用薄层色谱法对康赛德止痛液中陈皮、白术和党参进行了鉴别试验。鉴别方法灵敏，干扰少，重现性好。为控制其质量提供了依据。     

The Expression of Invasive Behavior of Differentiated Squamous Carcinoma Cell Line Evaluated by an in vitro Invasion Model

In order to elucidate the factors contributory to the expression of invasiveness of oral squamous cell carcinoma, we conducted biochemical and morphological comparisons of well differentiated squamous carcinoma cell line OSC-19 (oral squamous cell carcinoma) and undifferentiated carcinoma cell line KB, both cultured on 3T3 cell-embedded collagen gel ( in vitro invasion model). OSC-19 cells invaded 3T3 cell-embedded collagen gel, while KB cells and OSC-19 cells on 3T3 cell-free gel matrix were less invasive. Cultured OSC-19 cells were characterized by lower proliferating activity, lower secretion of laminin and higher secretion of fibronectin than those of KB cells. Although the basement membrane with deposition of laminin and type IV collagen was formed, it was discontinuous at the invasion front. Gelatin zymography and western blotting showed matrix metalloproteinases (MMP), i.e., 72 kDa gelatinase (MMP-2) and 92 kDa gelatinase (MMP-9). Gelatinolytic activity was assayed, and was higher in OSC-19 cells than in KB cells or OSC-19 cells of the 3T3 cell-free model. By immuno-histochemical analysis, MMP-2-positive cells were found scattered in both cell lines without any preferential localization, and the positivity for MMP-9 was localized in the invasion front of OSC-19 cells. These results strongly suggest that the invasiveness of squamous cell carcinoma is well correlated with cell-matrix adhesion by fibronectin and with focal elaboration of metalloproteinases, especially MMP-9, which play a major role in degrading the extracellular matrix components.     

龟鹿补肾丸和龟鹿补肾口服液对雄性生殖系统影响的研究

龟鹿补肾丸和龟鹿补肾液明显增加未成熟的小白鼠和去势大白鼠附性器官的重量;龟鹿补肾液能明显增强正常雄性和去势大白鼠的交配能力,随着剂量的加大,能提高未成熟大白鼠血清睾酮水平,而龟鹿补肾丸的影响不如龟鹿补肾液。     

止咳平喘口服液的药理研究

止咳平喘口服液灌胃给药能明显减少小鼠二氧化硫气雾引咳次数,增加小鼠气管酚红排泄量;对组织胺和乙酰胆碱收缩离体脉鼠气管、减少脉鼠离体肺、支气管灌流量的作用,有较明显对抗作用。     

鲁运神液的质量标准研究

对鲁运神液组成药物人参，党参，五味子，麦冬，鸡血藤进行了薄层鉴别，用比色法测定了人参皂甙Ｒｂ１的含量。     

益智灵对小鼠学习记忆的促进作用

采用一次性训练的被动回避反应—“Ｙ”电迷宫法，观察益智灵对亚硝酸钠和３０％乙醇致小鼠记忆障碍的改善作用，并与尼莫地平和神经生长因子的作用作了比较。３种药物对改善亚硝酸钠致记忆巩固障碍均有效。益智灵和神经生长因子还拮抗３０％乙醇致记忆再现障碍。另结果表明益智灵对正常小鼠学习行为有促进作用，其它两药未见有此作用。