The SCMC test: a reliable monitor for antispermatozoal antibodies

Seventeen couples (13%) were selected from a group of 129 infertilepatients according to the following criteria: (i) unexplainedinfertility for 3 years and (ii) <50% shaking spermatozoaduring SCMC testing. The couples were tested for sperm antibodiesafter a complete diagnostic work-up schedule. Post-coital testswere performed during the first menstrual cycle of the wife,followed by SCMC and sperm antibody titre testing. Ten malesand seven females were thus treated with 96 mg methylprednisolone.Nine (52%) of the 17 with sperm antibodies achieved a pregnancy.The results of the SCMC test were in all the cases indicativeof the actual sperm antibody titre. Reduction of the antibodytitre and a decrease in the percentage of shaking spermatozoaas detected by the SCMC test correlated well with the pregnancyrate amongst the patients.     

成年大鼠胰岛的体外基因转染

目的 探讨携带外源基因的慢病毒在体外有效感染胰岛及外源基因在胰岛中的表达，为通过移植前向胰岛细胞转入特定的免疫调节分子基因诱导胰岛移植物耐受奠定基础。方法 将目的基因CTLA4Ig导入慢病毒载体pWPTS，构建成pWPTS-CTLA4Ig载体。用磷酸钙沉淀法将pWPTS-EGFP、pWPTS-CTLA4Ig分别和其辅助载体pMD2．G、pCMVΔ8．92共转染293T细胞，收获病毒上清液，测定病毒滴度后感染新分离的胰岛。通过Western Blot测定胰岛培养上清液中CTLA4Ig蛋白的表达。结果 ①成功构建了携带CTLA4Ig基因的慢病毒载体pWPTS-CTLA4Ig；②包装产生的慢病毒Lenti-EGFP、Lenti-CTLA4Ig在体外可以感染胰岛，其中在Lenti-EGFP慢病毒感染的胰岛观察到了绿色荧光，及在Lenti-CTLA4Ig慢病毒感染的胰岛培养上清液中检测到了CTLA4Ig蛋白的表达。结论 慢病毒在体外可以有效感染大鼠胰岛，且携带的外源基因可以在胰岛细胞中稳定表达，其中Lenti-CTLA4Ig慢病毒感染的胰岛为进行胰岛移植并诱导体内特异的胰岛移植物耐受奠定了基础。     

昆明市儿童家庭动力学特征调查

目的 了解昆明市学校儿童家庭动力学特征.方法 采用家庭动力学自评量表和自编一般情况调查问卷对昆明市盘龙区城市、农村、城郊结合部3所学校四年级、五年级学生共928名进行现场集体调查,对家庭动力学特征及相关因素进行分析.结果 928名学生中,男孩475人(51.296),女孩453人(48.8%).儿童的性别对家庭气氛、系统逻辑、痰病观念维度的影响有显著差异(P＜0.05);家庭的生活环境对个性化、系统逻辑维度的影响有显著差异(P＜0.05);家庭结构(是否独生子女)对家庭动力学影响较小.结论 儿童的性别、家庭的生活环境对家庭动力学影响较为明显,而家庭结构(是否独生子女)对家庭动力学影响较小.     

主、被动音乐治疗对慢性精神分裂症疗效分析

目的　观察主、被动音乐治疗对慢性精神分裂症治疗效果。方法　慢性精神分裂症 72例 ,随机分为主、被动音乐治疗组 ,各 3 6例 ,观察 8周。以 BPRS、SANS量表评定疗效。结果　两组患者 BPRS、SANS量表评分差异均有显著性意义。结论　对慢性精神分裂症的康复治疗主动性音乐治疗优于被动性音乐治疗。     

A new method for assaying adhesion of cancer cells to the greater omentum and its application for evaluating anti-adhesion activities of chemically synthesized oligosaccharides

A new ex vivo method for assaying adhesion of cancer cells to the greater omentum has been developed using mouse greater omentum and [³H]labelled human gastric and mouse colorectal cancer cells. Since the adhesion rates were found to increase up to 18 h and labelled cells seemed to be stable during the period, the present method could be useful for investigating adhesion of cancer cells to the greater omentum, which must occur at the first step of the peritoneal dissemination. The adhesion of cancer cells to the greater omentum was inhibited by a series of chemically synthesized oligosaccharides and Galβ1,3[3OMeGalβ1,4GlcNAcβ1,6]αBn was found to be the best inhibitor. The anti-tumor effect of this novel tetrasaccharide in vivo was shown in preliminary experiments using Balb/c mice and colon26 cells.     

The Mainz Severity Score Index: a new instrument for quantifying the Anderson-Fabry disease phenotype, and the response of patients to enzyme replacement therapy

Anderson-Fabry disease (AFD) is an X-linked disorder caused by deficient activity of the lysosomal enzyme alpha-galactosidase A. The availability of enzyme replacement therapy (ERT) for this debilitating condition has led to the need for a convenient and sensitive instrument to monitor clinical effects in an individual patient. This study aimed to develop a scoring system--the Mainz Severity Score Index (MSSI)--to measure the severity of AFD and to monitor the clinical course of the disease in response to ERT. Thirty-nine patients (24 males and 15 females) with AFD were assessed using the MSSI immediately before and 1 year after commencing agalsidase alfa ERT. Control data were obtained from 23 patients in whom AFD was excluded. The MSSI of patients with AFD was significantly higher than that of patients with other severe debilitating diseases. The MSSI indicated that, although more men than women had symptoms classified as severe, overall, the median total severity scores were not significantly different between male and female patients. One year of ERT with agalsidase alfa led, in all patients, to a significant (p < 0.001) reduction in MSSI score (by a median of nine points). This study has shown that the MSSI score may be a useful, specific measure for objectively assessing the severity of AFD and for monitoring ERT-related treatment effects.     

银屑病生物治疗研究进展

 当前医学界一致认为，银屑病是一种 T 细胞引发并维持的自身免疫性疾病，病人机体免疫系统紊乱，健康细胞受到攻击，造成临床上典型的皮肤鳞屑性炎症病变^[1]。这种慢性炎症性疾病的病理过程包括T细胞浸润、真皮血管的改变、表皮角质细胞过度增生和异常分化 。其中参与银屑病发病的 T 细胞大致经历3个阶段的变化：T细胞（CD45RO+）的初始活化；T 细胞迁移至病变皮肤；活化T 细胞释放细胞因子发挥促增殖功能。真皮内的 CD4+ T细胞及其产生的细胞因子是银屑病发病的中心环节，角质细胞与血管内皮细胞的变化只是继发于细胞免疫机制异常的一种改变^[2]。随着对银屑病发病机制及免疫学机制研究的不断深入，人们陆续开发出多种生物制剂。这些生物制剂与传统的化学制药制造出来的具有严格化学分子式的小分子药品完全不同，它们都是蛋白质大分子，可以特异性作用于T细胞活化过程中不同的信号转导分子及途径，从而阻断疾病进程。此类生物制剂均通过生物工程技术获得，按性质大致可以划分为重组单克隆抗体（单抗）、重组抗体融合蛋白、重组细胞因子或生长因子等 3 大类；根据作用于 T 细胞活化靶点的不同，又可以分为针对原发性刺激、阻断共刺激分子作用、抑制T细胞增殖、抑制有炎症介导活性细胞因子和平衡有免疫调节活性的细胞因子等 5 大类^[3-4]。生物制剂的出现，为银屑病治疗提供了新的选择，正逐步改变着皮肤科医师治疗银屑病的传统模式。本文就近几年来银屑病生物治疗方面的最新研究进展做一综述。......     

Long-term inhibition of intimal hyperplasia using vascular photodynamic therapy in balloon-injured carotid arteries

Flexible treatments for intimal hyperplasia after angioplasty are still needed. The aim of this study was to demonstrate the long-term effects of vascular photodynamic therapy with talaporfin sodium on intimal hyperplasia following interventional injury. Intimal hyperplasia was induced by balloon distension injury to the carotid artery in 31 rabbits. Talaporfin, 5.0 mg/kg, was delivered systemically immediately after balloon injury. The injury site was irradiated with a diode laser light of wavelength 664 nm using a fluence of 50 J/cm² after 30 min. At day 3 and weeks 3, 6, 9, 15, and 25 after photodynamic therapy, the treated artery of each rabbit was excised and examined immunohistochemically. Thirty minutes after talaporfin administration, drug fluorescence was found only in the balloon-injured carotid artery wall. At 3 days, no smooth muscle cells were seen in the media of the photodynamic therapy-treated arterial segments. Intimal hyperplasia developed progressively in the balloon-injured and untreated segments; however, in the segments treated with photodynamic therapy, intimal hyperplasia was markedly suppressed until 25 weeks and the media was repopulated by smooth muscle cells without macrophages. Vascular photodynamic therapy with talaporfin may be used to inhibit restenosis after vascular intervention. An erratum to this article is available at .     

阴道镜下高频电灼术联合α-2a干扰素凝膏治疗尖锐湿疣疗效分析

目的探讨阴道镜下高频电灼术联合重组人干扰素α-2a治疗尖锐湿疣（CA）的效果。方法将165例CA分为3组，A组应用阴道镜下高频电灼术联合重组人干扰素α-2a；B组单纯采用阴道镜下高频电灼治疗；C组应用NS-FII型多功能光谱治疗仪联合肌注重组人干扰素α-2a。结果治疗后3-6个月A、B、C组复发率分别为0％、4．4％、65．4％：半年后人乳头瘤病毒（HPV）转阴率分别为93．5％、85．4％、43．8％，A组明显优于B组，B组明显优于C组，3组比较差异有统计学意义（P〈0．01）。结论阴道镜下高频电灼术联合重组人干扰素α-2a治疗CA可明显降低CA复发率和提高HPV转阴率。