精浆催乳素浓度与精子计数、活动率的关系

对107例男性进行精液分析并测定其精浆催乳素(PRL)浓度,精子活动率和精子密度正常男性的精浆PRL为11.53±5.36ng/ml,与精子活动率正常而少精子的男性相比(13.06±4.62ng/ml),两组间无显著性差异(t=0.91,t>0.2);而精子密度正常、正常精子活率组和低活率组相比,低活率组的精浆PRL水平(17.24 ±5.86ng/ml)明显增高(t=4.15,P<0.001).相关分析表明精浆PRL与精子活动率呈负相关(r=-0.38,P<0.001);与精子密度亦呈负相关(r=-0.37,P<0.001).49例受试者取精液前抽血测定血清PRL水平,精浆PRL水平与血清PRL无关(r=0.204,P>0.1).     

尿音图判断尿流异常的初步探讨

为探索一种新的、方便有效的诊断尿流异常的检查方法，采用尿音图对２例健康人及４例排尿异常患者（２例前列腺增生，１例尿道口畸形）的排尿声音录音后经语音图描记并进行分析。结果正常与３种异常排尿的尿音图明显不同。认为尿音图可望成为诊断尿道疾病的一种新的和有效的检查方法。     

Immunosuppression by human seminal plasma fractionated by DEAE Sephadex A-50 ion exchange chromatography

We fractionated the whole human seminal plasma on DEAE Sephadex A-50 ion exchange columns. Complete separation was achieved in seven peaks using different salt concentrations in phosphate buffer pH 6. The seminal plasma proteins were separated by sodium dodecyl-sulphate polyacrylamide gel electrophoresis. Human seminal plasma (SP) and its fractions were used in mixed lymphocyte reaction in vitro. Fractions 3, 4, and 7 were found to suppress the proliferation of human peripheral blood mononuclear cells to phytohemagglutinin and pokweed mitogen at a concentration of 10 micrograms ml-1 while stimulatory effect was observed at lower concentrations (1 microgram and 2.5 micrograms ml-1). Whole human SP and other fractions failed to suppress the proliferation of lymphocytes in vitro. Furthermore, the effect of human SP and its fractions was also investigated on phagocytic function of polymorphonuclear leukocytes (PMNs) using luminol dependent chemiluminescence assay stimulated with phorbol myristate acetate and opsonized yeast. Fractionated SP was found to have a suppressive effect on the luminol-dependent chemiluminescence of PMNs in the whole blood.     

结缔组织病的心脏表现

本文对确诊的96例结缔组织病(CTD)中52例心血管异常者(54.0％)进行分析;以往有高血压、糖尿病或其它心脏疾患者不列入本组。据临床表现、心电图、二维超声心动图及彩色多普勒(超声心动图)血流显像(CDFI)检测心脏异常:其中心包积液9例(17.3％),心肌炎22例(42.3％),心肌病3例(5.7％),瓣膜病变7例(13.3％)。二尖瓣脱垂4例(7.6％),冠状动脉病变1例(1.9％),心律失常22例(42.3％),心力衰竭7例(13.3％)。对7例无瓣膜功能不全或收缩功能不全的系统性红斑狼疮(SLE)以CDFI检测其左室舒张功能,结果IVRT延长(80±14 ms),A峰升高(66.3±6cm/s),E峰降低(65.1±8.0cm/s)及A/E>1.02±1.012者共5例,提示左室舒张功能不全,且发生于收缩功能不全之前。对3例CTD以CWD法,用简化Bernoulli公式p=4V~2+10计测肺动脉压,与右心导管检查结果肺动脉平均压各为4.7 kPa、5.8 kPa、6.7 kPa相符,分别诊为SLE、MCTD并发肺动脉高压。     

11;19 Translocation in a congenital leukemia with two cell populations of lymphoblasts and monoblasts

This report describes a case of a female infant of congenital leukemia with a chromosomal translocation t(11;19) (q23;p13) which presented initially with lymphoid features and at relapse with monocytic ones. The clinical course and the results of sequential cytochemical, cytogenetic and immunological studies are considered to be consistent with the interpretation of leukemogenesis of the myelo-monocytoid progenitor cell which still retains the capability of exhibiting lymphoid features to a limited extent. Although leukemia with t(11;19) has been classified as ANLL, most commonly M5 of FAB classification, the patients with this chromosomal abnormality may have a mixed leukemia in which cells with lymphoid features and those with monocytic ones exist.     

Rapid detection of euchromatin by Alu-PRINS: use in clinical cytogenetics

Alu-PRINS was successful in providing positive identification of euchromatin when oligoprimers were used at either extremity of the consensus Alu sequence. This technique was sensitive, as shown by the ability to detect small regions of euchromatin translocated to the short arm of an acrocentric chromosome. Since the Alu-PRINS technique is rapid and relatively simple, it will be useful in the diagnostic cytogenetics laboratory for the assessment of aberrant short arms of acrocentric satellites. This technique was also useful in defining the structure of small accessory marker chromosomes.This revised version was published online in November 2005 with corrections to the Cover Date.     

先天性主动脉异常的MRI诊断

本文分析１１例先天性主动脉异常的ＭＲＩ结果。其中主动脉缩窄、校正型大血管错位、右位主动脉弓及主动脉弓离分别为３、３、４和１例。ＭＲＩ是非常有效的检查方法，在先天性主动脉异常的诊断中有独到之处。本文还结合文献对ＭＲＩ、ＵＣＧ及ＡＣＧ进行比较讨论。     

男性不育患者精液干扰素水平的观察

本实验采用微量细胞病变抑制法,共检测44例正常生育男子精液标本和83例不育男性患者精液标本的干扰素水平.发现正常生育男子精液中的干扰素阳性率为18.18%,而不育男性患者精液中干扰素水平的阳性率为55.42%,两者有显著性差异(P<0.01).提示精液中干扰素的水平与男性不育有明显的关系.本文介绍一种新的精液干扰素检测技术,并为男性不育症的鉴别诊断提供新手段.     

Mosaic tetraploidy in a liveborn infant with features of the DiGeorge anomaly

We report on a liveborn male infant with mosaic tetraploidy who presented with multiple congenital anomalies including features of the DiGeorge anomaly (type I truncus arteriosus with other cardiovascular malformations, thymic hypoplasia, hypocalcemia). No structural chromosome aberrations, namely of chromosome 22, were detected. These findings contribute to the variability of symptoms of the polyploid phenotype. Additionally, the cytogenetic studies in our case emphasize the necessity of investigating fibroblasts in order to evaluate the relevant proportion of aberrant cells in mosaicism.     

Clinical and neuropathological features of a neurodegenerative disorder in the central nervous system with progressive head drooping (Kubisagari)

The clinical and neuropathological features of a case of a neurodegenerative disorder with pronounced and progressive head drooping, in Japanese Kubisagari, are reported. This female patient died at the age of 72 years after an approximately 20-year history of peculiar posture with progressive head drooping (Kubisagari) and lordosis (bowed posture), parkinsonism, dysphonia and slight muscle wasting of the face, tongue, neck, and distal portions of the upper extremities. She did not display mental deterioration until the terminal stage of the illness. A simple macroscopic inspection of formalin-fixed sections of the central nervous system (CNS) showed prominent atrophic frontal and temporal lobes, brownish discoloration of the putamen and an atrophic pyramidal tract. Light microscopy revealed severe neuron loss with fibrillary gliosis at both the above-mentioned lobes and the putamen. Both the facial and hypoglossal nuclei had almost disappeared. Motor neurons in the spinal cord were moderately to markedly decreased. Neither Bunina nor Lewy bodies, senile plaque, nor Pick's argyrophilic neuronal inclusions were observed, but very occasionally ubiquitin-positive neurons were found in the temporal cortex. In conclusion, the hitherto-unrecognized neuropathological findings in the CNS corresponding to progressive head drooping (Kubisagari) suggest that this is a neurodegenerative disorder of the CNS, possibly an atypical form of amyotrophic lateral sclerosis.