Frank压迫法阴道成形术治疗先天性无阴道

目的探讨Frank压迫法阴道成形术治疗先天性无阴道的临床效果。方法观察Frank法治疗159例先天性无阴道患者的治疗时间和随访治疗效果。结果159例患者全部治疗成功,所需时间最长7个月,最短10 d,62例已结婚,随访性生活满意。结论对于阴道前庭部弹性好,阴道外口处有0.5 cm以上陷凹的先天性无阴道患者,用Frank压迫法阴道成形术简便、安全、有效,有2 cm以上阴道者,疗效更佳。     

人类男性Y染色体变异对男性生育力影响的临床分析

目的:探讨Y染色体异常对男性生育能力的影响。方法:对因不育而就诊的4 238例男性患者进行G显带核型分析。结果:共检出Y染色体异常核型550例,占全部被检者12．98％,其中大Y 染色体异常497例;小Y染色体21例;倒位染色体13例;缺失Y染色体12例;47,XYY 4例;45,XO／ 46,XY嵌合体3例;48,XXYY 1例;环状Y染色体1例;Y染色体的平衡易位46,X,t(Y;17)(q12; q25)1例、46,X,t(Y;3)(q11;p11)1例、46,X,t(Y;14)(q12;q22)1例和1例46,X,t(Y;15) (p11;q13)。结论:Y染色体数目与形态结构的异常对男性生育能力有重要影响。     

抗精神病药物对心电图的影响分析

目的：探讨抗精神病药物与心电图异常的关系．方法：对342例住院精神病人用药后ECG变化进行分析，以性别、年龄、住院时间进行比较．结果：174例ECG异常，各种药物所致ECG异常的发生率无明显差异。〉40岁者较〈40岁者ECG异常改变的明显增高．结论：抗精神病药物均可引起ECG异常。用药期间应注意定期检查ECG．     

闭经患者的外周血淋巴细胞染色体观察

目的：探讨闭经患者与染色体异常的关系.方法：选择闭经患者139例（其中原发性闭经91例,继发性闭经48例）,对其进行外周血淋巴细胞染色体检查.结果：原发闭经组,性染色体数目和（或）结构异常者28例,常染色体异常1例.继发闭经组,性染色体数目和（或）结构异常者4例,常染色体异常1例.结论：结合临床体征和染色体检查,不仅为闭经患者寻找病因提供理论依据,而且有利于指导闭经患者的治疗.     

Ten‐year retrospective clinicohistological study of cutaneous lupus erythematosus in Korea

An understanding of the differences in clinical manifestations and laboratory abnormalities between subtypes of cutaneous lupus erythematosus (CLE) is still lacking. The purpose of this study was to analyze demographic, clinical and histological features of CLE according to three main presentation subsets: acute (ACLE), subacute (SCLE) and chronic (CCLE). A 10‐year retrospective analysis was performed on data from patients who were diagnosed with CLE between March 2005 and September 2015 in a Korean tertiary referral dermatology clinic. We compared demographic data and clinical and histological findings between three different CLE groups. An overall sample of 220 patients with CLE consisted of 67 patients with ACLE, 25 patients with SCLE and 135 patients with CCLE. Patients with CCLE regardless of systemic lupus erythematosus (SLE) presence had lower prevalence of anemia, urinary abnormalities and elevated erythrocyte sedimentation rate. Furthermore, CCLE patients who only had skin lesions showed lower female predominance, lower extracutaneous manifestation, fewer laboratory and immunological abnormalities including low antinuclear antibody titers and the lowest positivity for C3, C4 and anti‐dsDNA, anti‐Ro, anti‐Sm and anti‐RNP antibodies, and more prominent perieccrine inflammation and dermal fibrosis in histological findings. Considering distinct cutaneous manifestations of LE, a comprehensive awareness of each CLE subtype is important for achieving a favorable prognosis through appropriate diagnosis and management. This study provides comparative clinical and histological profiles of patients with different CLE subtypes in Korea.     

Intermedin inhibits norepinephrine-induced contraction of rat seminal vesicle

ObjectiveTo study the effect of inter medin(IMD) on smooth muscle of rat seminal vesicles including the specific receptors and the signal pathways involved.MethodsThe contraction of the seminal vesicle in response to norepinephrine (NE) and ADM2/IMD was studied by the organ bath method. The effects of antagonists for calcitonin gene related peptide (CGRP), adrenomedullin (ADM) and IMD receptors, and inhibitors of nitric oxide synthase, [L-NG-Nitroarginine Methyl Ester, L-NAME) and cAMP-dependent protein kinase (PKA), KT5720] were also investigated. The first overshoot, amplitude, frequency and basal tone were measured.ResultsThere is no significant effect of IMD on the initial overshoot, frequency and the basal tone in the seminal vesicle contraction. Only the amplitude of the contraction induced by NE was inhibited by IMD. The IMD inhibitory actions on amplitude were completely blocked by hADM22-52 and L-NAME, but not by hCGRP8-37 or KT5720. Furthermore, the action was diminished by IMD17-47.ConclusionThe results demonstrated that the inhibitory action of IMD on NE-induced seminal vesicle contraction was mediated via the ADM receptor(s) and the nitric oxide production pathway, partially by the IMD receptor, but not by the CGRP receptor and the cAMP-PKA pathway.     

慢性硬膜下血肿并发行为异常的影响因素分析

目的研究慢性硬膜下血肿并发行为异常的影响因素。方法回顾性连续纳入2009年1月至2019年10月四川省德阳市人民医院收治的慢性硬膜下血肿207例,将所有患者依据是否并发行为异常分为行为异常组及非行为异常组。收集患者一般人口学资料如性别、年龄及病史(如外伤史、吸烟史、饮酒史、脑血管病史、高血压病史、糖尿病史、血脂异常病史、凝血异常史),记录患者入院时格拉斯哥昏迷量表(GCS)评分,收集入院时CT及脑CT灌注等影像学资料,包括血肿量、血肿部位、血肿密度、CT中线移位、镰下疝、脑灌注缺损等资料。由专业的心身医学科医师进行精神检查,并进行汉密尔顿抑郁量表(HAMD)、修订韦氏成人记忆量表(WMS-RC)及力量表(WAIS-RC)测试。以HAMD评分>7分为抑郁状态,记忆商及总智商<79分作为记忆障碍和智能障碍的判断标准。采用Logistic回归分析影响慢性硬膜下血肿并发行为异常的独立危险因素。结果慢性硬膜下血肿患者并发行为异常的发生率为73.43%(152/207)。单因素分析结果显示,两组慢性硬膜下血肿患者在性别、年龄、凝血异常史方面差异无统计学意义(均P>0.05);两组患者在外伤史(χ^2=8.763)、吸烟史≥10年(χ^2=11.491)、饮酒史≥10年(χ^2=14.365)、脑血管病史(χ^2=11.492)、高血压病史≥10年(χ^2=13.057)、糖尿病史≥10年(χ^2=9.534)、血脂异常病史(χ^2=13.274)、病程≥3个月(χ^2=7.731)、血肿量≥30 ml(χ^2=12.763)、额颞部血肿(χ^2=21.458)、混杂密度血肿(χ^2=8.736)、CT中线移位≥5 mm(χ^2=14.572)、镰下疝(χ^2=10.396)、GCS<8分(χ^2=7.216)、额颞叶脑灌注缺损(χ^2=6.781)方面比较,差异均有统计学意义(均P<0.05)。Logistic回归分析结果显示,外伤史(OR=2.164,95%CI:1.083~2.934)、吸烟史≥10年(OR=2.346,95%CI:1.191~2.835)、饮酒史≥10年(OR=2.941,95%CI:1.284~3.157)、脑血管病史(OR=3.178,95%CI:1.893~4.597)、高血压病史≥10年(OR=2.783,95%CI:1.231~2.957)、糖尿病史≥10年(OR=2.841,95%CI:1.309~3.637)、有血脂异常病史(OR=3.237,95%CI:1.794~5.124)、病程≥3个月(OR=3.957,95%CI:1.997~5.463)、血肿量≥30 ml(OR=4.875,95%CI:1.982~5.875)、额颞部血肿(OR=4.763,95%CI:1.898~5.968)、混杂密度血肿(OR=4.537,95%CI:1.795~5.362)、CT中线移位≥5 mm(OR=4.876,95%CI:1.897~5.985)、并发镰下疝(OR=4.495,95%CI:1.754~5.247)、GCS<8分(OR=4.875,95%CI:1.897~5.876)、额颞叶脑灌注缺损(OR=4.237,95%CI:1.651~4.896)是影响慢性硬膜下血肿患者并发行为异常的独立危险因素。结论慢性硬膜下血肿并发行为异常受外伤史、吸烟史、饮酒史、脑血管病史、高血压病史、糖尿病史、高血脂病史、病程、血肿量、血肿部位、血肿密度、CT中线移位、镰下疝、GCS、脑灌注等因素影响,有针对性给予干预可能有效降低慢性硬膜下血肿发生行为异常的发生率。     

Effect of nutritional status on arsenic and smokeless tobacco induced genotoxicity,sperm abnormality and oxidative stress in mice in vivo

Background : Recently, high concentrations of arsenic have been documented in ground waters of Southern Assam, India. Indiscriminate smokeless tobacco consumption is a common practice in this region. Correlation between nutritional status and arsenic and smokeless tobacco‐induced health effects has not been taken up in humans or other test systems. Methods : Mice were divided into groups based on protein (casein) content in the diet: High protein (40%), optimum protein (20%), and low protein (5%). Simultaneous chronic exposure (90 days) to arsenic and smokeless tobacco (sadagura) orally was given to evaluate the extent of the cytological and genotoxicological damage. Micronucleus assay and Comet assay of the femur bone marrow cells were conducted. Germ cell toxicity was evaluated by recording the sperm head abnormalities and total sperm count. Cell cycle analysis was performed in femur bone marrow cells using flow cytometer. Hepatic, renal, and intestinal tissues were analyzed for various oxidative stress evaluations. Histological examination of liver and kidney was performed. Results : Notably, high protein diet groups had lower arsenic and sadagura induced genotoxicity, germ cell abnormalities and oxidative stress as compared to optimum protein and low protein diet counterparts. Conclusion : Our study indicates that sufficient levels of dietary protein appear to reduce the long‐term arsenic and smokeless tobacco‐induced toxicity in mice test system, as compared to lower or deficient amount of protein in the diet. This observation has implications and invites further studies especially epidemiological studies in the human population exposed to arsenic in South East Asian countries. Environ. Mol. Mutagen. 59:386–400, 2018. © 2018 Wiley Periodicals, Inc.