白附子不同炮制品抗炎作用比较研究

对白附子生、制品进行了抗炎作用的比较研究。结果表明,白附子对大鼠蛋清性、酵母性及甲醛性关节肿,有明显或不同程度的抑制作用。对棉球肉芽肿增生亦有明显的抑制作用,其抗炎作用同免疫器官胸腺,脾脏关系不大。新、老法制品与生品抗炎作用相近,新老法制品比较亦无差异。     

黄连炮制前后化学成分和生物碱含量对比研究

用薄层层析、紫外光谱和柱层紫外分光法对黄连(味连、雅连、云连)进行了炮制前后化学成分和总生物碱含量的对比,结果表明,无明显差异。     

六味地黄煎剂及其主药对环磷酰胺引起染色体损伤的保护作用

六味地黄煎剂(又称六味地黄汤)为常用补益剂,具扶正固本作用,文献报道能降低多种化学物质引起的实验肿瘤诱发率,临床上治疗消化道肿瘤的前期病变也取得较好疗效,表明六味地黄煎剂对肿瘤的发生有一     

中药材天南星中夏佛托苷的高效液相色谱法测定

目的:建立中药材天南星中夏佛托苷含量的高效液相色谱测定方法。方法:Shim-pack CLC-ODS分析柱(6.0 mm×150 mm,5μm),流动相为乙腈-0.025 mol·L~(-1)磷酸(15:85);柱温25℃;流速1.0mL·min~(-1);检测波长为270 nm。结果:线性范围为0.013～0.066μg,精密度试验RSD为0.81％,稳定性试验RSD为0.81％,重现性试验RSD为1.5％,回收率为96.5％,RSD为1.8％。结论:本方法快速、简便、准确、重现性较好,结果可靠,可为天南星药材的质量评价提供有效手段。     

微乳增敏分光光度法测定天麻制剂中的微量锌

本文研究了以十六烷基三甲基溴化铵（CTMAB）－正戊醇－正庚烷－水组成的阳离子型O／W微乳液为介质，以4－（2－吡啶偶氮）间苯二酚（PAR）为显色剂，用分光光度法直接测定天麻制剂中的微量锌含量，方法灵敏、准确、简便。其增敏效果与相应的胶束体系比较更加明显。     

A combined extract of Cinnamomi Ramulus, Anemarrhenae Rhizoma and Alpiniae Officinari Rhizoma suppresses production of nitric oxide by inhibiting NF-kappaB activation in RAW 264.7 cells

An herbal mixture prepared with Cinnamomi Ramulus, Anemarrhenae Rhizoma and Alpiniae Officinari Rhizoma (CAA) is used in oriental medicine for treating several ailments. The purpose of this study was to determine the mechanisms by which CAA elicits an antiinflammatory effect on nitric oxide (NO) production in the mouse macrophage cell line RAW 264.7 cells. The results indicated that lipopolysaccharide (LPS)-induced NO production was inhibited by CAA in a dose-dependent manner. Western blotting and RT-PCR analysis demonstrated that CAA decreased LPS-induced inducible nitric oxide synthase (iNOS) protein and gene expression in RAW 264.7 cells. Furthermore, CAA inhibited the LPS-induced DNA binding activity of nuclear factor-kappa B (NF-kappaB) and this effect was mediated through inhibiting the degradation of inhibitory factor-kappaBalpha (IkappaBalpha). Therefore, the results demonstrate that CAA inhibits LPS-induced production of NO and expression of iNOS by blocking NF-kappaB activation. CAA might be a potential therapeutic candidate for treating inflammatory diseases such as arthritis.     

白附子提取工艺的研究

目的:确定白附子美白成分的提取工艺。方法:采用正交设计法,以提取物产率、提取物对酪氨酸酶的相对抑制率为指标优选精制工艺。结果:微波功率为500W条件下加10倍量水,提取15m in。     

Antifibrotic effects of a herbal combination regimen on hepatic fibrotic rats

Liver fibrosis has been characterized as chronic inflammatory processes involving multiple molecular pathogenetic pathways. This therapeutic study investigated whether a combination regimen of Salvia miltiorrhiza (S), Ligusticum chuanxiong (L) and Glycyrrhiza glabra (G) exerted in vivo antifibrotic effects on rats with hepatic fibrosis. Fibrosis was induced in rats by dimethylnitrosamine (DMN) administration for 4 weeks. Fibrotic rats were randomly assigned to one of the three groups: control, SLG (50 mg/kg) or silymarin (50 mg/kg), each given by gavage twice daily for 3 weeks starting 1 week after DMN injection. The results showed that fibrosis scores of livers from DMN-treated rats with SLG (1.13 +/- 0.13) were significantly reduced in comparison with DMN-treated rats receiving vehicle (1.63 +/- 0.18). Moreover, the hepatic collagen content of DMN rats was significantly reduced by either SLG or silymarin treatment. The double immunohistochemical staining results also showed that alpha-SMA positive cells with NF kappa B nuclear translocation were decreased in the fibrotic livers by SLG and silymarin treatments. The mRNA expression levels of TGF-beta1, alpha-SMA, collagen1 alpha 2, iNOS and ICAM-1 genes were attenuated by SLG and silymarin treatment. The results showed that SLG exerted antifibrotic effects in rats with DMN-induced hepatic fibrosis.     

泽泻对免疫系统的影响及抗炎作用

泽泻煎剂10.20g/kg抑制小鼠碳粒廓清速率及DNCB所致接触性皮炎;泽泻20g/kg明显减轻二甲苯引起的小鼠耳廓肿胀、抑制大鼠棉球肉芽组织增生,而对血清抗体含量及大鼠肾上腺内抗坏血酸含量无显著影响。