经后路寰枢椎侧块螺钉固定治疗寰枢椎脱位

目的探讨经后路寰枢椎侧块螺钉固定治疗寰枢椎脱位的手术方法。方法采用后路枢椎侧块螺钉治疗6例寰枢椎前脱位。结果随访6～24个月,4例肌力完全恢复正常,2例肌力基本恢复正常,无断钉脱钉现象。结论经后路寰枢椎侧块螺钉固定治疗寰枢椎脱位,可使寰枢椎达到稳定的状态,该方法有效且可靠。     

二氧二茂钛原料药的稳定性研究

利用紫外分光光度计对二氯二茂钛进行含量测定。回归方程为 :Y =0 9881X - 0 90 90× 10 -3(Y :吸光度 ;X :浓度 ,mg/L) ,相关系数r=0 9999,线性范围为 13 .6～ 81.6mg/L。模拟试验 3份 ,回收率为 98 4 5 %～ 10 1 3 3 % ,相对标准偏差为 0 14 %～ 0 3 3 %。本实验研究了原料药二氯二茂钛的稳定性 ,结果表明不同的湿度、温度等条件对二氯二茂钛的稳定性有一定影响     

吸光系数法测定二氯二茂钛原料药的含量

采用吸光系数法对二氯二茂钛原料药进行含量测定。３９１ｎｍ处测得Ｅ１％１ｃｍ＝９８．３２,平均回收率为９８．７７％～１０１．７４％。相对标准差ＲＳＤ＝０．１２％～０．６６％。测定不同温度,湿度下放置不同时间原料药的含量。与工作曲线法相比。两者之间无显著性差异。     

基坑边坡变形和稳定性的离心试验研究

通过土工离心模型试验对上海某放坡式开挖基坑边坡的变形特性和稳定性进行了研究,揭示了基坑边坡变形的时间效应及其对边坡稳定性的影响。试验结果表明能长期稳定的边坡其边坡变形速率呈现出加速、接近匀速和衰减等3个明显的阶段;能瞬时稳定但不能长期稳定的边坡,其失稳发生在边坡位移速率增大的阶段。     

Positive SARS-CoV-2 RT-qPCR of a nasal swab spot after 30 days of conservation on filter paper at room temperature

We tested the use of nasal swabs spotted onto filter paper (Whatman 3M) for the molecular diagnosis of SARS-CoV-2 infection. Spots of a positive nasal swab in conservation medium (B.1.177 strain, 21Ct) were still positive (duo E-gene/IP4) after 10, 20, and 30 days of conservation at room temperature, with Ct values of 28, 27, and 26, respectively. Direct spotting of the swab at bedside (omicron strain) still gave a positive result after 10 days in two RT-qPCR systems: 33.7 Ct using duo E-gene/IP4, and 34.8 using a specific Omicron system. Spotting of a dilution range of media spiked with the Delta (strain 2021/FR/0610, lineage B 1.617.2) and Omicron strains (strain UVE/SARS-CoV-2/2021/FR/1514) showed a threshold of 0.04 TCID₅₀ after 10 days of conservation. We show, for the first time, that this simple and low-cost conservation method can be used to store samples for RT-qPCR against SARS-CoV-2 for up to at least 1 month.     

沈氏装置稳定性的生物力学评价

评价沈氏经椎弓根内固定装置的生物力学稳定性。方法采用５具新鲜尸体脊柱标本,制造Ｌ１损伤模型,测量并比较标本在完整状态,损伤状态、沈氏装置固定、沈氏装置疲劳及ＣＤ装置固定下的Ｔ１２和Ｌ２之间的三维运动范围,并将沈装置与ＣＤ装置固定下的运动范围进行,经统计学处理,评价沈氏装置的稳定性。     

Chloride Ion-Containing Polymeric Ionic Liquids for Application as Electrolytes in Solid-State Batteries

Organic, solid-state batteries require efficient solid electrolytes able to provide stable ion conduction. Here, solid electrolytes based on ionic liquid (IL) polymers with chloride counterions as electrolyte materials for batteries are presented. Acrylic monomers with imidazolium substituents with alkyl side groups that are linked by alkyl spacers to the acrylic group are employed. The IL monomers with chloride counterions are either converted by thermally initiated radical polymerization into linear homopolymers or incorporated into polymer networks by UV-initiated copolymerization utilizing a bifunctional, non-ionic cross-linker. Both procedures successfully yielded the desired materials, which is confirmed by NMR spectroscopy (linear homopolymers) or Raman spectroscopy (IL networks). The ionic conductivities at room temperature are measured by Electrochemical Impedance Spectroscopy. The ionic conductivities of the linear homopolymers are in the range of 10⁻⁴ to 10⁻⁶ S cm⁻¹, while those of the IL networks are about two orders of magnitude lower. They increase to 10⁻⁴ S cm⁻¹ at 70 °C. The electrochemical stability is examined by Linear Sweep Voltammetry and is proven in the voltage range of −2 to +2 V. The results reveal that the materials represent promising electrolytes for potential solid-state battery applications.     

Phosphorus-Containing Fluoro-Sulfonated Polytriazole Membranes with High Proton Conductivity: Understanding Microstructural and Thermomechanical Behaviors as a Function of Degree of Sulfonation

The current article describes the design and synthesis of a new series of phosphorus-containing fluoro-sulfonated polytriazoles through click polymerization. The synthesized copolytriazoles (PTPFDSH-70 to 90) with different degrees of sulfonation (DS) from 70% to 90% are structurally interpreted by various spectroscopic techniques (¹H, ¹³C, and FTIR). The high molecular weight (weight average molecular weight as high as 77 500 g mol⁻¹ with polydispersity index of 2.29) polymers exhibits excellent mechanical (elongation at break up to 95%), thermal (10% decomposition temperature: 266–317 °C), and oxidative (>14.5 h) stability. The PTPFDSH-70 to 90 possess outstanding water-holding ability in hydrated conditions (swelling ratio [in-plane]: 6.2–7.3% at 80 °C). The microstructural alterations by their thermal relaxations and transitions with increasing DS in the polymers have been thoroughly investigated by dynamic mechanical analysis. The atomic force microscopy and transmission electron microscope images of the PTPFDSH-70 to 90 polymer membranes demonstrated the phase segregated interconnected ionic cluster-like morphology between hydrophilic and hydrophobic domains. The PTPFDSH-90 (DEB:PFAZ:DSAZ = 100:10:90) polymer membrane displays the proton conductivity (176 and 190 ms cm⁻¹ at 80 and 90 °C, respectively) higher than Nafion117 under similar test conditions.     

Evaluation of Genetic Stability of Recombinant Human Factor VIII by Peptide Mapping and On-line Mass Spectrometric Analysis

Purpose. The genetic stability of a recombinant human factor VIII (rhFVIII) product expressed in Chinese hamster ovary cells (Recombinate) has been evaluated through comparisons of the protein produced at the beginning, middle and end of a typical production campaign. Methods. Recombinant human factor VIII was incubated with thrombin, the resulting four polypeptides were isolated by RP-HPLC, subjected to proteolysis with trypsin, and the peptide mixtures were resolved by RP-HPLC. Tryptic peptide mixtures were subjected to online mass spectrometric analysis using an electrospray ionization source interfaced to a quadrupole mass analyzer scanning from 1950–200 amu, and the peptide ion data were compared for three lots produced from the beginning, middle and end of a production campaign. Results. The UV elution profiles for each of the rhFVIIIa polypeptides were highly similar for factor VIII isolated from the beginning, middle and end of production. Total ion data from the peptide maps derived from three lots of rhFVIII were compared by MH¹⁺ values as a function of scan range. A total of 918 ions were analyzed for the four polypeptides of rhFVIII produced at the beginning, middle and end of a production campaign. The ions were detected at the same relative retention times, as indicated by the similar scan numbers for the three lots. Conclusions. These observations support that rhFVIII preparations produced from the beginning, middle and end of a production campaign were highly similar, and demonstrate genetic stability in the manufacturing process of Recombinate.