Genomic location of bovid herpesvirus type 2 nucleotide sequences homologous to five herpes simplex virus type 1 genes

The location of nucleotide sequences within the bovid herpesvirus 1 (BHV-2) genome homologous to herpes simplex virus 1 (HSV-1) DNA were investigated. BHV-2 DNA was digested with restriction endonucleases and blotted to nitrocellulose paper. The blots were then probed with plasmids containing HSV-1 genes for thymidine kinase (TK), the major DNA binding protein (ICP8), the major capsid protein (VP5) and genes for HSV-1 glycoproteins gB, gD, and gC. Except for HSV-1 gC, each HSV-1 gene tested hybridized to BHV-2 nucleotide sequences that were located either on both sides of a restriction endonuclease cleavage site, within a small restriction endonuclease fragment, or to an area common to two overlapping restriction fragments. Thus, we were able to localize BHV-2 nucleotide sequences homologous to the HSV-1 ICP8 gene between 0.38 and 0.41 map units (m.u.), and BHV-2 nucleotide sequences homologous to the HSV-1 VP5 gene between 0.24 and 0.27 m.u. In addition, BHV-2 nucleotide sequences homologous to HSV-1 genes for TK, gB and gD were found to lie on both sides of restriction endonuclease cleavage sites at 0.30 0.35, and 0.94 m.u., respectively.     

不同标测方法的右室流出道室性早搏射频消融治疗

目的比较不同标测方法的射频消融治疗右室流出道室性早搏的疗效和安全性。方法66例右室流出道室性早搏随机分组(起搏标测组和起搏+最早激动点联合标测组)进行射频消融治疗。结果起搏标测组和联合标测组各33例,两组分别成功消融32例,总成功率为96.8%,无严重并发症出现。但起搏标测组和联合标测组的手术及X线曝光时间不一致,分别为(72.87±23.34)vs.(58.56±20.56)min(P<0.05)和(7.59±2.67)vs.(6.13±1.71)min(P<0.05)。结论射频消融治疗右室流出道室性早搏疗效确切,安全性良好,起搏和最早激动点联合标测可缩短手术及X线曝光时间。     

A consensus approach: Understanding the support needs of women in Newport West,Wales, to participate in breast screening

Introduction

Breast screening is an effective way to improve the early detection of breast cancer and reduce mortality. Unfortunately, low uptake of screening is often reported. This study aimed to explore the support needs of women residing in Newport West, Wales, to participate in breast screening.

Methods

Group Concept Mapping, a structured participatory consensus approach, was used as the method. Participants completed three activities either online or offline: brainstorming to generate statements, sorting statements into themed categories; rating statements for perceived importance and accessibility (easy to get).

Results

Thirty-seven participants from seven ethnic groups took part. Sixty-three statements (items of support) were generated and sorted into seven conceptually similar clusters (themes) (Trusting that I will be respected; Reassurance about my experience; Accessibility and convenience; Practical support; Addressing cultural diversity; Information tailored to individual needs; Raising awareness and understanding of breast screening). The ‘Trusting that I will be respected’ cluster was rated most important, while the ‘Practical support’ cluster was rated least accessible. Some disparity between responses was found based on ethnicity, language, disability and previous attendance of breast screening.

Conclusions

Women require a range of support to participate in breast screening. The results highlight the importance of ensuring women feel and are respected, instilling trust in the staff performing the screening, offering reassurance about positive experiences of breast screening and providing practical support, especially individualized/targeted support for people who do not speak and/or read English and those with a disability.

Patient or Public Contribution

The public contributed to the development of the information sheet, consent form, recruitment and data collection method.