萘普生缓释胶囊的药代动力学和生物利用度研究

目的 通过健康受试者的双交叉试验比较萘普生缓释胶囊与普通片的药物动力学和生物利用度。方法１０位健康受试者一次服用这两种制剂５００ｍｇ，在稳态试验中８位受试者接受两种制剂５天，缓释胶囊５００ｍｇ每天一次，普通片每天两次，每次２５０ｍｇ。血药浓度用ＨＰＬＣ方法测定。结果 一次服药试验证明该缓释胶囊血药浓度上升缓慢，浓度变化平稳，Ｇｍａｘ为８５．９μｇ／ｍｌ，Ｔｐｅａｋ为６．０ｈ，而普通片的Ｃｍａｘ为１４０．４μｇ／ｍｌ，Ｔｐｅａｋ为３．２ｈ，两种制剂的生物利用度相当，缓释胶囊与普通片生物等效。通过８位健康受试者交叉连续服用萘普生缓释胶囊与普通片的稳态药代动力学研究表明，到第３天给药后已达到稳态，由第５天给药后的血药浓度测定数据计算其主要的稳态药代动力学参数，缓释胶囊的峰浓度（Ｃｍａｘ）、峰谷比和波动度（ＤＦ）明显低于普通片，这些参数分别为７３，１μｇ／ｍｌ、１．４８和３７．７ｏ；而普通片的上述参数分别为 １００．８μｇ／ｍｌ、２．５１和７８．２％。结论 奈普生缓释胶囊具有缓释作用，能较好维持血药治疗浓度。     

Kinetic study of glomerular epithelial cells associated with segmental glomerular sclerotic lesions with adhesion in spontaneously diabetic WBN/Kob rats

Background We previously found that glomerular epithelial cells play an important role in the formation of adhesive lesions. Glomerular sclerotic lesions develop after the inital adhesive lesions. Methods Two series of experiments were done with spontaneously diabetic WBN/Kob rats. These rats develop segmental glomerular sclerotic lesions with aging. The first series of experiments was intended to clarify the kinetics of glomerular cells on progressive glomerular damage in these rats. The second series of experiments was designed to study the relationship between proliferation (judged by % bromodeoxyuridine-positive cells) of glomerlar epithelial cells and sclerotic lesions with adhesions. Results In the first series, rats having increased proteinuria showed segmental glomerular sclerotic lesions with adhesions. At the same time, increased labeling indices of tuft cells and epithelial cells of Bowman's capsule were observed. In the second series, no significant increase in the labeling indices of tuft cells with sclerotic lesions was observed, compared to tuft cells without sclerotic lesions. In sclerotic lesions with adhesion, bromodeoxyurdine-positive cells were observed that were not distinguishable as podocytes or epithelial cells of Bowman's capsule. The highest labelling index was noted in the epithelial cells of Bowman's capsules with sclerosis. Conclusion This study shows that the proliferation of glomerular epithelial cells (mainly epithelial cells of Bowman's capsule) occurs in glomerular sclerotic lesions with adhesions.     

救心胶囊对LDL损伤内皮细胞抗氧化损伤能力影响的实验研究

[目的]观察救心胶囊对低密度脂蛋白(LDL)损伤后内皮细胞抗氧化损伤能力的影响,以探讨该复方保护内皮细胞和防治冠心病的作用机制。[方法]运用内皮细胞培养技术,建立LDL损伤模型,并以两种不同的给药方式——无菌中药提取液和载药血清进行分组培养,并观察内皮细胞超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的变化。[结果]内皮细胞LDL损伤后,其SOD活性显著降低,而MDA含量明显升高,而中药处理后能有效抑制上述改变。[结论]救心胶囊能增强细胞的抗氧化损伤能力,具有良好的保护内皮细胞的作用。     

Joint capsule matrix turnover in a rabbit model of chronic joint contractures: Correlation with human contractures.

To evaluate changes in matrix molecules of the joint capsule, the right knees of 24 skeletally mature female NZW rabbits were immobilized while the contralateral limb served as an unoperated control. The immobilization was discontinued at 8 weeks and the rabbits were divided among four groups (n = 6) based on the number of weeks the right knees were remobilized: 0, 8, 16, or 32. Three rabbits (six knees) that did not have operations provided normal control joint capsules. The mRNA levels for collagen types I, II, and III, and MMP-1 and -13 were significantly increased in the joint capsules of the contracture knees in all groups when compared to normal and contralateral limb joint capsules. In contrast, the mRNA levels for TIMP-1, -2, and -3 were decreased in the joint capsules of the contracture knees in all groups when compared to normal and contralateral limb joint capsules. The mRNA levels for lumican and decorin were increased in the joint capsules of the contracture knees in all groups when compared to normal capsules. Many of the changes observed in this animal model are similar to those observed in human joint capsules from posttraumatic elbow contractures, supporting the value of this rabbit model.     

疏可眠胶囊镇静作用的实验研究

且的:观察疏可眠胶囊对小鼠的镇静作用。方法:采用旷野活动法观察疏可眠胶囊不同给药剂量、给药时间对小鼠自主活动的量效、时效作用;对士的宁诱发惊厥小鼠的作用。结果:连续给药7d,疏可眠低中剂量(1g·k~(-1)、2g·kg~(-1))均能显著减少小鼠的自主活动;疏可眠高剂量(3g·kg~(-1))给药后1h～2h自主活动显著减少;给药后2h,呈现量效关系,随着剂量增加小鼠自主活动减少。各剂量组均可延长士的宁诱发小鼠惊厥出现的时间及死亡时间。结论:疏可眠胶囊具有减少小鼠自主活动和抗惊厥作用。     

Recurrent locking of knee joint caused by intraarticular migration of bioabsorbable tibial interference screw after arthroscopic ACL reconstruction

Two cases are reported in which, after ACL reconstruction with autologous hamstring grafts, tibial polylactide interference screws migrated into the knee joint. Clinically, both patients presented with recurrent locking of the joint. In one case, a broken 15 mm-long tip of the screw was found intra-articularly. In the other case, the whole screw had migrated into the joint cavity. The degradation process of polylactic acid, operative technique and bone quality are discussed as possible reasons for these complications.     

HPLC法测定复方五仁醇软胶囊中芍药苷的含量

目的 测定复方五仁醇软胶囊中芍药苷的含量.方法 采用色谱柱ODS-C18,流动相:乙腈-水(15∶85),检测波长230nm,流速1.0ml/min.结果 芍药苷在0.05～0.80 mg·ml-1范围内线性良好,r=0.999 9,平均回收率为97.93%(n=5),RSD为1.68%.结论 本方法重现性好、简便易行,可作为复方五仁醇软胶囊中芍药苷含量的质控方法.     

头风痛胶囊治疗头风病(偏头痛)风瘀证临床观察附:62例病例报告

目的　观察中药四类新药头风痛胶囊治疗头风病 (偏头痛 )风瘀证的临床疗效及安全性。方法　采用随机对照试验 ,将 6 2例病人随机分为治疗组和对照组 ,每组 31例 ,分别服用头风痛胶囊和头风痛丸 ,疗程 1月 ,观察其疗效和副作用。结果　治疗组的头痛疗效为显效率 6 4 5 2 %、有效率 87 10 % ;中医证候疗效为显效率 6 1 2 9%、有效率 90 32 %。两组疗效之差异无统计学意义 (P >0 0 5 )。临床试验期间未发现头风痛胶囊所致的不良反应。结论　头风痛胶囊有较好的祛风止痛作用 ,服用安全 ,与头风痛丸具有相同的疗效 ,是头风痛丸的理想新剂型     

重建钛板枢椎椎弓根螺钉及颗粒状植骨枕颈融合术

目的 探讨重建钛板螺钉及颗粒状自体松质骨植骨在枕颈融合中的应用。方法 2002年4月～2005年1月，选择枕颈区不稳定患者19例，年龄31～67岁；病程3个月～2年。其中枕寰枢椎复合畸形8例，陈旧性寰枢椎骨折脱位8例，类风湿性关节炎所致寰椎前脱位2例，枢椎齿状突肿瘤1例。JOA脊髓功能评分平均9．8分。使用重建钛板和枢椎椎弓根螺钉固定枕颈部，同时枕骨与枢椎后弓间颗粒状自体松质骨植骨。结果 术中、术后无并发症发生，切口Ⅰ期愈合。19例均获随访6个月～2年8个月，平均16个月，均获得了骨性融合。无神经血管损伤，无断钉、断板及内固定松脱。JOA脊髓功能评分平均达14．4分。结论 重建钛板枢椎椎弓根螺钉固定可靠，置入方便，自体颗粒状松质骨具有较高的融合率，在枕颈融合中效果满意。     

通心络对颈动脉粥样硬化患者脑血流动力学的影响

目的　研究颈动脉粥样硬化伴脑供血不足患者 ,用通心络治疗前后血流动力学的变化。方法　给予 53例有颈动脉粥样硬化的脑供血不足的患者服用通心络胶囊 4粒 ,每日 3次 ,疗程为 4周。治疗前后采用经颅彩色多普勒 (transcranialdoppler,TCD)检测颅内各血管的血流变化 ,并与 30例老年健康体检者进行对照。结果　脑供血不足组各血管平均血流速度 (Vm)与健康对照组比较明显降低 (P <0 .0 5或 P <0 .0 1 ) ,且多数血管搏动指数 (PI)升高 (P <0 .0 5) ;治疗后脑供血不足组各血管的Vm较治疗前有显著性增高 (P <0 .0 5) ,且PI较治疗前有显著性降低 (P <0 .0 5)。结论　通心络可改善颈动脉粥样硬化患者的脑供血 ,是治疗和预防脑供血不足的有效药物