Axonal neuropathy in a patient with monoclonal IgM kappa reactive with Schmidt-Lantermann incisures

We report a patient with a progressive, predominantly sensory neuropathy and a IgM kappa M-protein that binds to Schmidt-Lantermann incisures. A sural nerve biopsy showed primary axonal damage and IgM deposits at Schmidt-Lantermann incisures were seen by direct immunoperoxidase. Serum from the patient injected into rat sciatic nerve reacts with the incisures as with those in the patient's nerve. The IgM kappa M-protein reacts with chondroitin sulfate C and binds to a broad nerve protein band with a mobility of between 170 and 118 kDa. Peripheral neuropathy may be related to the M-protein, which had immunocytochemical reactivity not previously described for patients with polyneuropathy and IgM monoclonal gammopathy.     

多糖硫酸脂(PSS)治疗缺血性脑血管病临床观察和实验室研究

应用PSS治疗270例缺血性脑血管病并进行了临床、实验室以及动物试验的观察.总有效率为92.27%;显效率65.24%,与对照组相比较有显著性差异(P<0.005).实验室观察发现PSS有显著的抗凝血、降低血粘度,降低血脂及血管扩张作用.动物实验也说明了这一点.     

Simplified determination of serum cholesterol sulfate by gas-liquid chromatography combined with cyclohexylsilane-bonded phase column purification

Summary A new gas-liquid chromatographic (GLC) determination of cholesterol sulfate (CS) and dehydroepiandrosterone sulfate (DHEAS) for a biochemical diagnosis of recessive X-linked ichthyosis (RXLI) is described. Although the GLC method for determination of CS is known to be more sensitive than the thin layer chromatographic (TLC) method, the former method has not been widely employed because of its complicated pre-purification steps. The present method allows us to measure the serum levels of CS and DHEAS without tedious purification steps such as multiple conventional column chromatography and preparative thin layer chromatography. Sulfated steroids are rapidly purified with a commercially available mini disposable cyclohexylsilane-bonded phase (CH) column, CH BOND ELUT, and the purified steroids after desulfation are converted to water-resistant tert-butyldimethylsilyl ether derivatives for the GLC analysis on dual 2 m glass columns packed with 2% XE-60 on Chromosorb W.By the present method, serum CS concentrations in RXLI patients were shown to be about 10 times higher than those in patients with ichthyosis vulgaris, carriers of RXLI, and healthy subjects. This method is more suitable not only for a biochemical diagnosis of RXLI but also for studies on the metabolism of sulfated steroids than the previous time-consuming GLC methods.     

尼莫地平片溶出度的研究

采用转篮法对尼莫地平国产市售片，进口片和自制片在不同介质、不同转速和ＰＨ条件下的溶出度进行测试。结果表明，介质对尼莫地平溶出具有很大的影响，而最佳测定方法是以０．１％的十二烷基硫酸钠水溶液为介质，转速１５０ｒ／ｍｉｎ。     

荞麦花粉抗贫血作用的实验研究

用荞麦花粉的水提取液进行了抗大白鼠缺铁性贫血的研究，结果表明：荞麦花粉具有和硫酸亚铁相似的抗缺铁性贫血作用．饮用荞麦花粉提取液３５ｄ的大白鼠，生长发育良好，主要脏器未见损害．该研究为荞麦花粉的开发利用提供了实验依据．     

硫酸镍对红细胞毒性研究

健康小鼠腹腔注射硫酸镍10.0,5.0,2.5mg/kg,导致外周血中红细胞增高,血红蛋白不变或降低,红细胞中点彩红细胞和碱粒红细胞占有比值明显增高,网织红细胞含量升高、提示镍对外周血红细胞有毒性损害。     

离子色谱法测定硫酸软骨素的含量

建立了用离子色谱法测定硫酸软骨素的方法。在６ｍｏｌ／Ｌ盐酸介质中将硫酸软骨素水解生成等摩尔的硫酸根离子，然后用ＡｎｉｏｎＨＣ离子色谱柱进行分离，以硼酸钠／葡萄糖酸钠淋洗液为流动相，流速１．５ｍｌ／ｍｉｎ，电导检测器检测。硫酸根离子在０．００１～０．０８０ｍｇ／ｍｌ浓度范围内线性关系良好，相关系数为０．９９９４，最低检测浓度为０．０００６ｍｇ／ｍｌ。样品中硫酸根离子的加样回收率为９８．５１％～９９．５２％，硫酸软骨素原料药和注射液及鲨鱼软骨粉胶囊中硫酸软骨素测定的相对标准偏差分别为０．２５％，１．０６％和１．９６％。该方法已应用于多种硫酸软骨素制剂中硫酸软骨素的含量测定     

硫酸锌对肠缺血—再灌注损伤的防治作用

目的：探讨硫酸锌对肠缺血－再灌注（ischemia-reperfusion,I/R)损伤的防治作用及其机制。（2）方法：复制家兔肠I／R损伤模型，观察硫酸锌对超氧化物歧化酶（SOD），黄嘌呤氧化酶（XO）及丙二醛（MDA）含量的影响，实验分为I／R损伤组，硫酸锌＋I／R组和假手术组（Sham)对照组，并进行比较。（3）结果：缺血前3组动物红细胞SOD、红细胞膜及血浆MDA，血浆XO值均无统计学差异，I／R组再灌注后2h与缺血前及Sham组相比，SOD活性显下降，XO活性及MDA含量明显增加（P＜0．01），此外，肠、肺、肝等组织MDA含量亦明显高于Sham组（P＜0．01），静脉给予硫酸锌，可使XO及MDA含量降低，且可防止SOD减少。结论：肠I／R过程中伴有多器官脂质过氧化损伤，硫酸锌通过抗脂质过氧化能减轻或在一定程度上避免再灌注损伤的进一步加剧。     

硫酸小诺霉素注射液的紫外分光光度测定

采用紫外分光光度法测定硫酸小诺霉素注射液的含量，以邻苯二醛为衍生化试剂，测定波长３３３ｎｍ。在２￣２０ｕ／ｍｌ范围内呈线性关系，ｒ＝０．９９９９。平均回收率为１００．０％，ＲＳＤ为０．８２％。本法简便、快速，结果准确。