不同剂量运脾方对脾虚大鼠小肠功能的影响

目的观察治疗儿童厌食症的有效方药运脾方不同剂量对脾虚大鼠小肠功能的影响,探索运脾方临床治疗效果的作用机理。方法建立利血平致脾虚动物模型,设立正常组、模型组、阳性对照组(消食健儿糖浆组)、运脾方大剂量组、运脾方中剂量组、运脾方小剂量组,测定大鼠体重变化及食量消耗,观察大鼠尿D-木糖排泄率。结果运脾方能明显增加脾虚大鼠的食量和体重,增加D-木糖的排泄率。结论运脾方能够改善脾虚大鼠小肠吸收功能,并呈量效关系。     

脾虚证分度定量诊断研究

通过对９０例多系统多病种脾虚患者的研究，采用多指标探索脾虚证的定量诊断方法。发现随着木糖吸收率的逐步降低，脾虚证各种虚状的出现率不断升高；除周围血管阻力渐上升外，唾液淀粉酶活性、血液流变学检测、心功能、血红细胞、血红蛋白及淋巴细胞转化率等指标值均逐渐下降，并与木糖吸收率呈正相关，有显著性意义。按照上述规律，将脾虚证分为Ⅰ、Ⅱ、Ⅲ３度，并列出各度的症状出现率及检测结果，初步提出脾虚证分度定量诊断法。     

227例肝郁脾虚证患者小肠吸收功能障碍的分析

本文报告277例不同病种肝郁脾虚病人尿 D—木糖测定。实验组测定值显著低于健康人对照组;其中123例进行了疏肝健脾方药治疗,并作疗程前后对比观察。结果显示,疏肝健脾方药具有提高小肠吸收功能的作用。     

INFANTILE MALNUTRITION AND MALABSORPTION

Recovery from severe protein-calorie malnutrition in Guatemalan children was associated with a rapid improvement in intestinal absorption of nitrogen, D-xylose, and vitamin A, and a slower improvement in absorption of fat, triolein, oleic acid, and vitamin B₁₂, which were closely associated with the protein-nutritional status of the child.     

黄芪多糖对脾虚水湿不化模型大鼠血清GAS、CCK、VIP及尿D木糖排泄率的影响

目的:观察黄芪多糖对脾虚水湿不化模型大鼠血清胃泌素(GAS)、胆囊收缩素(CCK)、血管活性肠肽(VIP)及尿D木糖排泄率的影响,探讨黄芪多糖健脾祛湿机制。方法:将48只大鼠随机分为模型组和空白组,将模型组大鼠按高脂低蛋白加力竭游泳法建立脾虚水湿不化大鼠模型,模型组分为参苓白术散组,黄芪多糖低、中、高剂量组,每组8只。按分组情况分别进行干预,观察模型大鼠一般状况,检测GAS、CCK、VIP水平及5 h尿D木糖排泄率。结果:与模型组比较,黄芪多糖各组血清GAS、CCK、VIP水平均升高(P0.05或P0.01),5 h尿D木糖排泄率增加(P0.05)。结论:黄芪多糖可纠正脾虚水湿不化大鼠胃肠激素水平及尿D木糖排泄率,进而改善饮食物消化吸收,发挥健脾祛湿作用。     

开郁健脾法对脾虚大鼠血清胃泌素、尿D-木糖影响的实验研究

目的观察具有开郁健脾功效中药复方对脾虚大鼠体质量、血清胃泌素、尿D-木糖排泄率的影响。方法以破气苦降加饥饱失常的方法建立脾虚证大鼠模型,随机分组,实验组中药复方煎剂灌胃,以ELISA法测定大鼠血清胃泌素,间苯三酚法测定D-木糖排泄率。结果 (1)与模型组比较,中药复方低、中、高剂量组体质量明显增长(P0.01)。(2)与模型组比较,中药复方低、高剂量组、中剂量组尿D-木糖排泄率显著增高(P0.05、P0.01)。结论开郁健脾中药复方有促进胃肠吸收的作用。     

麸炒苍术不同提取物对小鼠脾虚泄泻的影响

目的探究麸炒苍术水提物、醇提物、二氯甲烷部位对脾虚泄泻模型小鼠的治疗作用。方法选用番泻叶水煎液灌胃制备大鼠脾虚模型。考察麸炒苍术水提物、醇提物、二氯甲烷萃取物对脾虚小鼠体质量、腹泻等级、粪便含水率、血清淀粉酶、D-木糖水平及肠内短链脂肪酸(SCFAs)含量的影响,并做肠道病理学进行观察。结果与模型组比较,麸炒苍术水提物、醇提物、二氯甲烷部位均可显著减轻腹泻等级、缓解脾虚泻引起的体质量增长迟缓,降低粪便含水率,增加血清D-木糖及肠内短链脂肪酸含量,减轻肠黏膜损伤,此外麸炒苍术二氯甲烷部位组血清淀粉酶水平较模型组显著提高。结论麸炒苍术水提物、醇提物、二氯甲烷部位均能明显拮抗番泻叶所致小鼠脾虚泻,麸炒苍术二氯甲烷部位对于脾虚泻引起消化吸收功能降低能起到最好的改善作用。     

Intestinal permeability in patients with chemotherapy-induced stomatitis

Purpose: Mucositis represents one of the most common side effects of chemotherapy, and may affect any part of the gastrointestinal tract, resulting in stomatitis, dysphagia, dyspepsia, or diarrhea. The aim of the present study was to evaluate intestinal permeability in patients with stomatitis during treatment with oral granulocyte-monocyte colony-stimulating factor (GM-CSF, Leucomax). Methods: Ten patients with chemotherapy-induced stomatitis and 21 control cancer patients were included in the study. Intestinal permeability in patients with stomatitis was evaluated before and after the treatment with oral GM-CSF (200 μg for 4 consecutive days) by measuring urinary lactulose, D-xylose, and mannitol after oral challenge in collected urine using capillary gas chromatography. Results: Mean grade of stomatitis (3, range 2–3) improved during treatment by a mean of 1 grade (range 0–2, sign test P < 0.05) with an improvement observed in eight of ten patients. Lactulose excretion, lactulose/mannitol, and lactulose/xylose ratios were markedly elevated in the patients with mucositis compared with 21 control cancer patients (1.60 ± 1.04%, 0.2446 ± 0.2937, and 0.3877 ± 0.6808 vs 0.35 ± 0.20%, 0.0332 ± 0.0148, and 0.0255 ± 0.0086, respectively, Mann Whitney U-test, P < 0.001). After treatment, lactulose excretion, lactulose/mannitol, and lactulose/xylose ratio decreased significantly (1.60 ± 1.04 vs 0.63 ± 0.42%; 0.2446 ± 0.2937 vs 0.1303 ± 0.1149; and 0.3877 ± 0.6808 vs 0.1126 ± 0.1146, respectively, P < 0.05). Conclusions: Lactulose excretion after oral challenge, lactulose/mannitol, or lactulose/xylose ratio may be useful markers for intestinal involvement in chemotherapy-induced mucositis. Improvement of oral mucositis was associated with a significant decrease of intestinal permeability to lactulose. Testing of intestinal permeability by the present method may be useful to evaluate the effect of therapeutic interventions in patients with chemotherapy-induced mucositis. Received: 13 July 2000 / Accepted: 29 August 2000     

小儿厌食症尿D—木糖排泄率观察

研究发现厌食症病儿的尿D—木糖排泄率大多偏低,服用养胃生津滋脾的小儿厌食合剂后,尿D—木糖排泄率明显升高(P<0.001)。初步认为该试验除可作为脾气虚的客观指标外,尚可作为脾胃阴津亏虚的客观指标。     

自拟运脾和胃汤治疗小儿厌食症60例疗效观察

目的:观察运脾和胃汤治疗小儿脾胃不和型厌食症疗效。方法:选择符合诊断标准的患儿随机分为中药组、对照组各30例。中药组口服运脾和胃汤,对照组口服吗丁啉,两组连用7d,观察治疗前后两组患儿临床疗效及运脾和胃汤对尿D-木糖排泄率、微量元素的影响。结果:中药组较对照组总有效率、尿D-木糖排泄率明显升高(P<0.01);中药组治疗后较治疗前微量元素锌明显增高,有显著性差异(P<0.01)。结论:运脾和胃汤治疗小儿脾胃不和型厌食症疗效确切。