尿素循环障碍患儿慢性期治疗和管理

尿素循环障碍（UCD）是一组致死、致残率较高的遗传代谢病，需要长期饮食和药物治疗及管理。除希特林蛋白缺乏症和行肝移植治疗的患儿，其他慢性期患儿均需要终身低蛋白饮食，保证其相应年龄的安全蛋白质摄入量以及充足的碳水和脂肪的供能比，必要时补充必需氨基酸及无蛋白奶粉；药物治疗主要包括氮清除剂（苯甲酸钠、苯丁酸钠、苯丁酸甘油酯）、尿素循环激活/底物补充剂（N-氨基甲酰谷氨酸、精氨酸、瓜氨酸）等。规范饮食及药物治疗后未达预期效果、出现严重进展性肝病或出现反复发作的患儿建议行肝移植。基因疗法、干细胞疗法和酶替代疗法等新技术可能是UCD患儿治疗的新选择。UCD患儿需要定期检测血氨、肝功能和血氨基酸等生化指标，并评估体格生长、智力发育和营养摄入情况，及时调整治疗方案。     

Advancement of Krüppel-like factor 4 in tumor

KLF4为锌指蛋白转录因子,含特异性转录激活区和转录抑制区,体内分布广泛,在不同的肿瘤组织中,通过抑制细胞增殖、调节细胞分化、细胞凋亡和靶基因的表达,发挥着抑制或促进肿瘤形成的双重作用.其机制可能与乙酰化修饰有关.     

药用豆磷脂的HPLC法测定

本文报告了用HPLC法对药用豆磷脂进行定性定量分析。作者选用硅胶为固定相,以正己烷-异芮醇-醋酸盐缓冲液(pH5.8)(7.5:8:1)为流动相,紫外检测波长206nm,使得磷脂酰胆碱(PC),磷脂酰乙醇胺(PE),溶血磷脂酰胆碱(LPC),磷脂酰肌醇(PI)和磷脂酸(PA)获得满意分离,并对主成分PC进行了含量测定,平均回收率为99.8%,方法的变异系数为0.8%.     

99Tcm-MIBI显像检测乳腺癌多药耐药

探讨了乳腺癌组织的多药耐药现象的主要机制及P-糖蛋白(P-gp)、胎盘型谷胱甘肽硫转移酶(GST-π)与~(99)Tc~m-MIBI显像的关系。尽管文献报道的实验结果不尽相同，但作为一种功能显像技术，~(99)TC~m-MIBI SPECT显像能够用来研究乳腺癌细胞P-gp的表达，可以在体外无创性检测乳腺癌多药耐药，为指导乳腺癌的治疗提供参考。     

11C-胆碱和18F-FDG PET反映兔VX2肺肿瘤增殖活性的对比研究

objective Tumor proliferative activity has been recognized as an indicator of malignant degree in lung cancer and related to prognosis.The purpose of this study was to evaluate the feasibility of assossing proliferative activity with 11C-choline and 18F-fluorodeoxyglucose(FDG)PET on a rabbit beating lung VX2 tumor model.Methoils About 0.5 ml of viable VX2 tumor cell suspension was slowly injected into the right lungs of 54 New Zealand white rabbits through a transthoracical needle insertion.11C-choline and18F-FDG PET scan were performed 10-11 d after tumor implantation.One ear vein was cannulated for administration of the tracem.11C-choline PET scan(with Discovery LS PET/CT scanner,GE)was performed 5 min after intravenously injection of 37 MBq11C-choline.Then 18.7 MBq18F-FDG was infused at 60 min after11C-choline administration and18F-FDG PET scan was performed at 60 min after18F-FDG administration.The maximal standardized uptake value of tumor was calculated.The animals were euthanized after examination.Histochemical stain with proliferating cell nuclear antigen(PCCNA)was performed and PCNA index was obtained to assess tumor prolireration.The difference of11C-choline and18F-FDG was analvzed using paired student t-test.The correlation of 11C-choline 18F-FDG and tumor cell density and PCNA index was analyzed using Pearson Iinear regression.Results Of the 54 rabbits.36 had a solitary pulmonary tumor.The rate of successful generation of a solitary VX2 tumor was 66.7%(36/54).Only 33 rabbits underwent both 11C-choline and 18F-FDG PET,and enrolled in this study.The mean cellular density was (547.36±64.78) cells/field and the mean PCNA index was(42.34±15.26)%.18F-FDG was hisher than 11C-choline(5.70±3.45 vs 4.02±3.07,t=-3.188,P=0.003).11C-choline significantly and positively correlated with PCNA index(r=0.786,P＜0.05).However,there was no significant correlation between 11C-choline and tumor cellular density(r=-0.176,P=0.327).18F-FDG significantly and positively correlated to PCNA index(r=0.531.P=0.001).There was no significant correlation between 18F-FDG and cellular density(r=-0.105.P=0.561).Conclusions In a rabbit bearing lung VX2 tumor model,11C-choline or 18F-FDG uptake correlates with PCNA index.Thus PET scan with these two tracers may reflect tumor proliferative activity.11C-choline PET may be more reliable to evaluate tumor proliferation than 18F-FDG PET.     

复方甘草酸苷注射液联合多烯磷脂酰胆碱注射液治疗酒精性肝炎临床疗效观察

目的：观察复方甘草酸苷注射液联合多烯磷脂酰胆碱注射液治疗酒精性肝炎的临床疗效。方法：121例酒精性肝炎病人被随机分为两组，治疗组以复方甘草酸苷注射液联合多烯磷脂酰胆碱注射液治疗，对照组应用复方甘草酸苷注射液治疗，疗程为4周，观察治疗前、后血清肝功能（STB、ALT、AST、GGT）的变化。结果：治疗组与对照组总有效率分别为85％、63％（P〈0．01），且治疗组对肝功能指标的改善较对照组更为明显（P〈0．05）。两组均未见明显不良反应。结论：复方甘草酸苷注射液联合多烯磷脂酰胆碱注射液治疗酒精性肝炎疗效较好且安全。     

华支睾吸虫mGST新基因的克隆、表达与纯化

目的扩增华支睾吸虫一个微粒体谷胱甘肽转移酶（mGST）新基因,明确是否存在内含子,并进行原核表达与纯化. 方法用PCR方法分别从华支睾吸虫成虫cDNA（质粒）文库和基因组DNA中扩增mGST基因并测序,将编码基因定向克隆到原核表达载体pET-30a（＋）,在大肠埃希菌BL21/DE3中表达,按照Ni-NTA agarose说明书纯化重组蛋白,用SDS-PAGE和TLC分析. 结果 mGST基因全长486 bp,没有内含子,构建的原核表达质粒pET-30a（＋）-mGST在大肠埃希菌中得到了有效表达,融合蛋白的分子质量单位约为24 ku.重组蛋白达细菌总蛋白质的11%,纯化后的重组蛋白纯度为83%. 结论 mGST基因没有内含子,在大肠埃希菌中能有效表达,得到了纯化的重组蛋白,为进一步研究其功能奠定了基础.     

p21^WAF1的调节——乙酰化和甲基化

近来研究认为下调转录的DNA启动子区甲基化和使染色质功能下降的组蛋白乙酰化是p21^WAF1失活的重要机制。现综述组蛋白乙酰化和DNA甲基化对p21^WAF1转录的影响，以及与之有关的影响途径或因素。     

苯接触人群中GSTμ基因的多态性分析

探讨苯中毒患者GSTμ 基因缺失频度 ,为进一步研究GSTμ 基因缺失与苯白血病之间的关系寻找线索。方法采用聚合酶链反应(PolymeraseChainReaction,PCR)技术检测GSTμ 基因型。结果对照组、苯接触组、轻度苯中毒组、中度苯中毒组、重度苯中毒组GSTμ 基因缺失频率分别为46.88 %、51.79 %、53.75 %、56.00 %、69.84 %。进行2×K表的 χ2 检验结果显示:χ2=9.6555 ,P<0.05 ,说明苯接触者中毒的程度与GSTμ 基因缺失有关。结论GSTμ 基因缺失可能是苯中毒的危险性因素之一。