仙人掌提取物对小鼠血糖的影响

目的 :探讨仙人掌水性和酸性提取物对小鼠血糖的影响。方法 :糖尿病小鼠造模成功后分为 5组 :仙人掌酸提物 2个剂量组 (0 .5g·kg-1·d-1和 1.0g·kg-1·d-1) ,水提物 2个剂量组和优降糖组 (2 0 0mg·kg-1·d-1)。正常小鼠灌注等量生理盐水作为正常对照。 6组小鼠均连续给药 2 1d后测血糖。结果 :给药后仙人掌酸提物 2个剂量组血糖均较给药前降低 (P均 <0 .0 5 ) ,2个剂量组间差异无统计学意义 (P >0 .0 5 ) ,但 2组与优降糖组相比 ,P均 <0 .0 5 ;水提物 2个剂量组给药前后血糖无明显变化 (P >0 .0 5 )。结论 :仙人掌酸提物有降血糖作用     

Effect of various stabilizing agents on Imperata cylindrica grass pollen allergen extract

BACKGROUND: Allergen extracts are unstable, heat labile or susceptible to proteases. Stability of allergen extracts is important for proper diagnosis and therapy of allergic disorders. OBJECTIVE: The present study was undertaken to determine the preservation and stabilization conditions of Imperata cylindrica (Ic) grass pollen extract. METHODS: The Ic extract was kept with 0.1 mepsilon-aminocaproic acid (EACA), 0.75 m sucrose, 5% glycerol, 0.03% human serum albumin (HSA) or 0.4% phenol for different time periods. The extracts were stored for 3, 6 and 12 months each at 4 degrees C, 4 degrees C with daily exposure to room temperature (RT) for 1 h, and RT. The quality of extracts was analysed by SDS-PAGE, Western blot, ELISA, ELISA inhibition and skin test. RESULTS: Extracts kept with EACA and sucrose retained most of the protein bands followed by glycerol as determined by SDS-PAGE and Western blot during all storage periods and conditions in comparison with standard extracts. The extracts kept with HSA, phenol and without preservative (WP) showed protein degradation below 33 kDa after 3 months storage at all conditions. However, a 67-kDa allergen was stable in these extracts. EACA extract required 75 to 120 ng of protein for 50% inhibition in IgE binding under different conditions, whereas standard extract required 70 ng for the same. ELISA also demonstrated high allergenic reactivity of EACA extract. ID test on allergy patients with EACA extract demonstrated same allergenic potency as that of standard extract. CONCLUSION: EACA is the best preservative/stabilizing agent of Ic pollen extract, followed by sucrose and glycerol. Ic extract kept with phenol, HSA and without preservative showed degradation within 3 months. EACA preserved extract is equally potent as that of standard extract up to 1 year's storage.     

蝌蚪提取液诱导人早幼粒白血病细胞分化的实验研究

目的 :探讨蝌蝌提取液 (T871)对白血病细胞的诱导分化作用。方法 :利用细胞生长曲线、形态学观察、细胞免疫化学及原位mRNA杂交和完整细胞原位斑点印迹技术 ,观察了T871诱导HL 60细胞分化的作用和分化过程中癌基因c myc、c myb表达的变化。结果 :T871能抑制HL 60细胞的增殖 ,形态学表现为类似单核 /巨噬细胞 ,同时T871还能使HL 60细胞的NBT还原能力、ANAE活性显著增强 ,同时伴有癌基因c myc、c mybmRNA表达的下调。结论 :T871能诱导HL 60细胞向单核 /巨噬细胞方向分化 ,c myc、c myb癌基因可能参与了此过程     

正常人与重症肌无力患者胸腺提取液免疫活性比较

用~３Ｈ－ＴｄＲ掺入法观察不同稀释度的正常人与重症肌无力患者胸腺提取液对健康成人外周血淋巴细胞增殖的影响。实验证实了正常儿童的胸腺功能下降（高浓度Ｐ＜０．０２，余浓度Ｐ＞０．０５），成人胸腺无功能（Ｐ＞０．０５）；并且得出增生型重症肌无力胸腺提取液对ＰＨＡ诱导的人外周血淋巴细胞增殖有促进和抑制双向作用。提示重症肌无力患者的胸腺免疫活性与正常人比较有差异。解释并肯定了手术切除胸腺治疗增生型重症肌无力的作用。     

荔枝壳红色素的提取及稳定性观察

以荔枝壳为原料，在酸性条件下浸提荔枝壳红色素，并对其稳定性进行了研究，结果表明，该色素在酸性条件下对光、热、常用食品添加剂均具有较好的稳定性。     

酵母抽提物超滤制备海藻糖工艺条件的优化

利用响应面分析法对影响酵母抽提液中总氮截留率和海藻糖透过率的主要因素进行分析,优化超滤工艺,得到最佳超滤条件:料液质量浓度10 g／dL,料液温度42℃,操作压力0.31MPa,在最佳超滤条件下得到酵母抽提物蛋白质截留率96.5%,海藻糖透过率94.8%,所得RSA图可直观地反映各因素与蛋白氮截留率和海藻糖透过率的关系.     

鸡矢藤提取物对小白鼠离体子宫的作用

鸡矢藤[Paederia Scandens]提取物0.32mg 加入营养槽内,对小白鼠各期离体子宫的收缩张力、收缩强度、收缩频率以及子宫活动力均有增强作用。与给药前比较,诱导期组分别增加85%、47%、21%和72%;动情期组分别增加70%、77%、41%和41%;非动情组分别增加47%、76%、62%和66%,而且三组间作用无显著性差异。本品还能增强 PGE_2对小白鼠离体子宫的收缩张力、收缩强度、收缩频率以及子宫活动力。与 PGE_2的作用比较,分别增加34%、39%、9%和45%,但收缩频率增加无统计学意义(P>0.05)。上述结果表明,鸡矢藤提取物对小鼠子宫有显著性兴奋作用,而且还能增强PGE_2对小鼠子宫兴奋作用,其机制尚待进一步研究。     

银杏叶提取物中的芦丁含量测定

用紫外分光光度法测定银杏叶提取物中芦丁的含量，结果表明：以６０％丙酮或７０％乙醇提取银杏叶，所得提取物的芦丁含量为３３．２７％～４３．３８％。     

添喂纯植物提取物"益绿素"添加剂对奶牛生产性能的影响

选择体重、年龄、胎次、产乳量基本一致的荷斯坦泌乳奶牛10头,随机分为两组,分别为对照组(空白)和试验组;试验组每头奶牛每天一次补充10g添加剂。结果显示,添喂益绿素添加剂的试验组与对照组相比日产标准乳提高7.16%(P<0.01),奶料比提高7.31%(P<0.01),经济效益提高17.0%。表明添喂益绿素添加剂能有效提高奶牛产奶量,经济效益明显。     

猕猴桃中药复方制剂对血清SOD及MDA含量的影响

猕猴桃中药复方制剂系采用猕猴桃汁为基质，配加绞股蓝、丹参等中药的有效成分制成。为了研究它的保健作用，在６３例５０岁以上、无急性疾病的中、老年人，３６例３０～４９岁健康的青年，３２例１４～１７岁健康的少年中进行试验，１０ｍｌ每日２次连续服用３０天后，中老年人血液ＳＯＤ显著增高，７０岁以上老人平均增高２．８倍，血清ＭＤＡ明显下降，７０岁以上平均下降８７．５％。