自制苏芪合剂联合治疗Ⅲ～Ⅳ级IgA肾病的临床疗效观察

目的 探讨自制苏芪合剂对Ⅲ～Ⅳ级IgA肾病患者的疗效及患者T细胞功能的改善.方法 28例Ⅲ～Ⅳ级IgA肾病患者随机分为两组,对照组:醋酸泼尼松-日1mg,kg,隔日晨起顿服,盐酸苯那普利-日10mg;治疗组:在上述用药的基础上加用自制苏芪合剂.8周后,观察患者缓解率、T细胞功能及不良反应.结果 治疗组患者在缓解率、T细胞功能改善等方面优于对照组,且因糖皮质激素的使用而出现的不良反应少于对照组.结论 苏芪合剂联合治疗Ⅲ～Ⅳ级IgA肾病疗效显著并能有效改善T细胞功能.     

Complete response, as determined by prostate-specific antigen level, to chlormadinone acetate withdrawal persisting longer than 2 years in patients with advanced prostate cancer: Two case reports

Antiandrogen withdrawal syndrome (AWS) is a well-established phenomenon in prostate cancer. However, responses to AWS are usually of limited duration, and a complete response (CR) is extremely rare. We present two patients who exhibited a chemical CR for more than 2 years after the discontinuation of steroidal antiandrogen chlormadinone acetate use. Whether patients who respond to antiandrogen withdrawal include a group of patients with a better prognosis remains uncertain. However, considering that the usual survival period of patients with hormone-resistant prostate cancer is approximately 12 months, both of the patients reported here, who are present in excellent physical condition, exhibiting an improved quality of life, and attending their hospital as outpatients, obviously acquired a prolonged survival because of AWS.     

Prognostic significance of cytochemical analysis of leukemic M2 blasts

Cytochemical analysis of leukemic blasts from 46 patients with acute myeloblastic M2 leukemia (according to the FAB classification) was performed before and after cytostatic therapy, and compared with findings obtained in 20 age- and sex-matched control subjects. Cytochemical findings for myeloperoxidase (MPO), Sudan black B, acid phosphatase and alpha-naphthyl-acetate esterase (ANAE) were related to the achievement of the first complete remission (CR),i.e. data were compared after the patients had been divided into CR and non-CR groups. The analysis clearly showed that a high proportion of myeloperoxidase- and, to a lesser extent, Sudan black B-positive blasts before treatment may have constituted a significantly unfavourable prognostic factor.     

醋酸棉酚诱导膀胱癌T24细胞凋亡的研究

目的　研究棉酚对膀胱癌T2 4 细胞的抑制作用和诱导凋亡作用 ,探讨其作用机制。方法　将不同浓度的醋酸棉酚作用于人T2 4 膀胱癌细胞 ,利用MTT法检测其有效作用浓度、瑞氏染色、流式细胞仪 ,观察T2 4 细胞的凋亡情况。结果　MTT法测得其IC50 值为 12 6.3 μmol·L-1,浓度为 5 0～ 3 0 0 μmol·L-1的棉酚具有诱导T2 4 细胞凋亡的作用 ,随着药物作用时间的延长凋亡率增加。结论　棉酚抗癌作用机制之一是诱导人T2 4 膀胱癌细胞凋亡     

EFFECTS OF PROGESTOGENS ON LIPEMIA AND LIPOLYSIS IN RAT: EFFECTS OF PROGESTERONE, MEGESTROL ACETATE, NORETHINDRONE ACETATE, AND NOMEGESTROL ACETATE

Effects of 4 progestogens at equal dosages (5 mg/kg/day) on lipid metabolism and plasma glucose levels of adult female rats were compared. The 4 progestogens studied were progesterone (P) by SC injection, and megestrol acetate (MEG), norethindrone acetate (NOR), and nomegestrol acetate (NOM) PO. MEG, a 17 alpha-hydroxyprogesterone derivative, induced significant increases in glucose, total and high-density lipid (HDL) cholesterol, triglyceride, and phospholipid plasma levels. Treatment with NOR, a 19-nortestosterone derivative, resulted in a reduced gain in body wt and in a marked decrease in all plasma lipid parameters. The 19-norprogesterone derivative NOM, like P, did not alter lipid or glucose metabolism, despite a significant increase in body wt gain. In particular, no reduction in the HDL cholesterol level occurred. Plasma and tissue lipolytic activities remained unchanged. The results of this study confirm interest in the therapeutic class of 19-norprogesterone-derived progestogens, exemplified by NOM, with respect to their lack of metabolic side effects.     

重症肌无力患者泼尼松治疗前后免疫学指标的变化

探讨泼尼松治疗重症肌无力（MG）免疫学机制。对382例MG患者在漏尼松中剂量冲击，小剂量维持疗法治疗前后，检测酰胆碱受体抗炎（AchRab)，突触前膜抗体（PsMab)，单个核细胞亚群（PBMC），肿瘤坏死因子（TNF），可溶性白介素－2受体（SIL－2R），β2－微球蛋白（β2－m)，以及红细胞免疫功能的变化。结果表明：MG患者在泼尼松治疗前后多项免疫指标有显著性的变化。为泼尼松治疗MG提供了评定疗效的免疫学指标，进一步阐明了MG发病的免疫学机制。     

纯化的抗乙酰胆碱酯酶单克隆抗体对乙酰胆碱酯酶活性的影响(英文)

本文研究了Balb/c小鼠杂交瘤细胞分泌的抗乙酰胆碱酯酶单克隆抗体对电鳐电器官乙酰胆碱酯酶催化反应的影响及其机理。小鼠腹水抗体经蛋白A琼脂糖亲和层析分离纯化,达到聚丙烯酰胺凝胶电泳纯。酶联免疫吸附试验检测了11株单克隆抗体,滴度在1:5000～1:300 000范围内。在以乙酰胆碱为底物的催化反应中,3F3、2G8及1H11三株杂交瘤分泌的单克隆抗体对乙酰胆碱酯酶活性有明显的抑制作用,抑制作用随单克隆抗体用量的加大而增强。但在以乙酰靛酚为底物的催化反应中,仅3F3显示抑制作用。因而推论,3F3作用在乙酰胆碱酯酶的活力中心,而2G8及1H11可能作用在活力中心的邻近部位.乙酰胆碱酯酶的活力中心不仅是酶的催化部位,而且也是酶分子上的一个抗原决定簇.乙酰胆碱酯酶活力中心具有催化及诱导抗体产生的双重功能.     

血浆甲地孕酮的高效液相色谱测定法

本文研究和建立了测定血浆中甲地孕酮的反相高效液相色谱法。其最低检测限为2～4ng,一日内回收率为107%,三日内回收率为98%。本法与 RIA 法测定同一样品,结果相关性好,r=0.986。     

美替诺龙醋酸酯的合成

目的　由 17β 乙酰氧基 5α雄甾 1 烯 3 酮 (1)经 5步反应合成蛋白同化激素美替诺龙醋酸酯 (17β 乙酰氧基 1 甲基 5α雄甾 1 烯 3 酮 ,6 )。方法　 17β 乙酰氧基 5α雄甾 1 烯 3 酮 (1)在酸的存在下与NBS反应 ,得2 β 溴 1α ,17β 二羟基 5α 雄甾烷 3 酮 17 醋酸酯 (2 ) ,化合物 (2 )用H2 /活性镍催化氢解 ,得 1α ,17β 二羟基 5α 雄甾烷 3 酮 17 醋酸酯 (3) ,然后在对甲苯磺酸的催化下化合物 (3)与甲醇作用 ,得 3,3 二甲氧基 5α 雄甾烷 1α ,17β 二醇 17 醋酸酯 (4 )。化合物 (4 )经铬酐 硫酸氧化 ,得 17β 乙酰氧基 3,3 二甲氧基 5α 雄甾烷 1 酮 (5 ) ,再经格氏反应和乙酰化反应 ,制得 17β 乙酰氧基 1 甲基 5α 雄甾 1 烯 3 酮 (美替诺龙醋酸酯 ,6 )。由化合物 1到 6的总收率为 48.7%。结果　此法以较高收率合成了美替诺龙醋酸酯。结论　该合成法有适用于工业化生产的前景     

聚醋酸乙烯酯与铜（Ⅱ）离子的配位反应及其催化作用

以ＥＳＲ、ＵＶ和ＩＲ表征Ｃｕ（２＋）离子与ＰＶＡＣ的配位反应。根据ＣＵＣｌ２·２Ｈ２Ｏ乙醇溶液与ＰＶＡｃ－ＣｕＣｌ２·２Ｈ２Ｏ乙醇溶液的电导率差值随Ｃｕ（２＋）离子摩尔浓度变化的明显转析点得知，１个Ｃｕ（２＋）离子大约能与ＰＶＡｃ４个链节单元配位。证实ＭＭＡ在Ｃｕ（Ⅱ）－ＰＶＡｃ配合物／Ｎａ２ＳＯ３体系的聚合体是无规ＰＭＭＡ，得率为７０％。讨论了ＭＭＡ在Ｃｕ（Ⅱ）－ＰＶＡｃ配合物／Ｎａ２ＳＯ３体系络合催化引发下的游离基反应历程。