Scanning laser polarimetry with variable corneal compensation in migraine patients

Purpose: This study aimed to compare scanning laser polarimetry measurements of retinal nerve fibre layer (RNFL) thickness in eyes of migraine patients with those in eyes of age‐matched, healthy subjects. Methods: The study was designed as an observational, prospective, cross‐sectional study. It included 57 eyes of 57 patients with migraine with or without aura according to the criteria of the International Headache Society and 44 eyes of 44 age‐matched healthy controls. Scanning laser polarimetry images were obtained using a commercial GDx VCC system (Version 5.3.1; Laser Diagnostic Technologies, Inc.). At each sitting, three sets of GDx VCC measurements were acquired for each patient and used in the analysis. Image acquisition was performed in undilated eyes in all subjects. Results: The mean ± standard deviation RNFL average thickness parameter in the migraine subjects was significantly lower than in the control group, at 50.4 ± 4.8 μm versus 54.7 ± 3.4 μm, respectively (p < 0.0001). However, there were no differences between migraine subjects and controls in mean RNFL thickness in superior and inferior areas. In the migraine group the mean migraine disability assessment (MIDAS) score was 34.3 ± 15.3 and the mean number of attacks per year was 17.1 ± 6.9 (range 6?28). The mean RNFL average thickness parameter was significantly correlated with MIDAS score (r = ? 0.86, p < 0.0001) and frequency of attacks (r = ? 0.86, p < 0.0001). Conclusions: The mean RNFL average thickness parameter was found to be thinner in migraine patients. In addition, we found a strong correlation between migraine severity and RNFL average thickness parameters.     

The influence of posterior capsular opacification removal on anterior segment birefringence parameters as measured by scanning laser polarimetry

PURPOSE: To determine the effect of posterior capsular opacification (PCO) removal on anterior segment birefringence (ASB) and its influence on peripapillary retinal nerve fibre layer (RNFL) retardation measurements obtained by means of scanning laser polarimetry. METHODS: In this prospective interventional study, scanning laser polarimetry was performed using GDx variable corneal compensation on 26 eyes of 26 patients who developed clinically significant PCO (after uncomplicated cataract surgery and with no other ocular pathology) both before and between 1 and 4 weeks after Nd:YAG capsulotomy. Best-corrected visual acuity (BCVA), intraocular pressure, corneal polarization axis (CPA), corneal polarization magnitude (CPM) were compared using the Student t-test and Wilcoxon signed ranks test. Spearman correlations between changes (differences between values after and before capsulotomy) in the CPA, CPM, BCVA and RNFL data were also performed. RESULTS: PCO removal is associated with a shift in CPA (from 10.86 to 15.03 degrees, P = 0.004) and CPM (from 28.54 to 37.92 nm, P = 0.004). Significant correlations were found between changes in the parameters of ASB and BCVA. Furthermore, RNFL measurements (nerve fibre indicator, temporal-superior-nasal-inferior-temporal average and superior average) were also well related to the CPA and CPM shifts. CONCLUSIONS: PCO induces an inaccurate compensation of ASB which affects RNFL assessment. Thus, it is necessary to recompensate ASB after posterior capsulotomy.     

旋光法测定甲磺酸培氟沙星葡萄糖注射液中葡萄糖含量

目的用旋光法测定甲磺酸培氟沙星葡萄糖注射液中葡萄糖含量。方法直接取样测定甲磺酸培氟沙星葡萄糖注射液的旋光度,计算葡萄糖含量。结果葡萄糖质量浓度在0.03～0.07g/mL范围内与旋光度线性关系良好,线性回归方程Y=47.473X＋0.0246,r=0.9998（n=5）;平均回收率为100.28%,RSD为0.51%（n=9）。结论旋光法简便、准确、可靠,可用于甲磺酸培氟沙星葡萄糖注射液的质量控制。     

复方环丙沙星喷鼻剂含量测定方法研究

目的测定复方环丙沙星喷鼻剂中乳酸环丙沙星、盐酸麻黄碱的含量。方法用紫外分光光度法测定乳酸环丙沙星含量,溶剂为0．05mol／L氢氧化钠溶液,测定波长为334nm;用旋光法测定盐酸麻黄碱。结果乳酸环丙沙星质量浓度在6～18μg／mL范围内与吸收度呈良好的线性关系。乳酸环丙沙星、盐酸麻黄碱平均回收率分别为99．64％和100．27％,RSD分别为0．42％和0．58％（n=6）。结论方法简便、准确、灵敏度高、重现性好,可用于该制剂的质量控制。     

DL-泛内酯酶法拆分中检测方法及其自发水解

目的建立快速简便的泛内酯、泛解酸的检测方法,方便酶法拆分DL-泛内酯过程中监测,研究pH对DL-泛内酯自发水解的影响。方法建立了用硅胶薄层色谱法以碘蒸汽为显色剂进行薄层色谱检测泛内酯和泛解酸;结合HPLC法建立了以旋光法评价酶法拆分DL-泛内酯反应程度的新方法;考察pH值对混旋泛内酯自发水解的影响。结果与结论薄层色谱和旋光测定的方法适合酶法拆分DL-泛内酯反应程度的快速检测;混旋泛内酯的自发水解随pH增加而增加,而且在一定pH值下,自发水解随着时间的延长而增加。     

Effect of peripapillary chorioretinal atrophy on GDx parametersin patients with degenerative myopia

Purpose: To determine the effect of peripapillary chorio­retinal atrophy in degenerative myopia on the retinal nerve fibre layer (RNFL) thickness measurements performed by scanning laser polarimetry. Methods: Polarimetric RNFL analysis was done in 41 degenerative myopic eyes of 25 patients with spherical refractive errors between ?7.50 and ?22.00 D (mean ?12.50 D; SD 3.5). Analysis was also performed on 46 eyes of 24 age‐matched healthy subjects with spherical refractive errors between ?1.00 and +1.00 D (mean ?0.25 D; SD 0.50) with GDx Nerve Fiber Analyzer (Version 1.0.12). All of the myopic patients had clinically evident peripapillary chorio­retinal atrophy in their fundus examination. Results: In myopic patients most of the GDx parameters were statistically different from the control group (P < 0.05). Although average thickness, ellipse average, number, inferior maximum and inferior average were found to be higher than the healthy subjects, the modu­lation parameters and the ratio‐based parameters were significantly decreased in degenerative myopic patients (P < 0.05). The TSNIT (temporal, superior, nasal, inferior, temporal) graph showed irregular high spikes with loss of the regular double hump pattern, especially in quadrants with large chorioretinal atrophy and visible sclera. Conclusion: Peripapillary chorioretinal atrophy increased retardation values, which might be explained by the high reflectivity of the bare sclera in these areas.     

联合应用多种分析方法测定复方苯甲酸散的含量

目的:测定复方苯甲酸散中各组分的含量,建立一套完整、简便、有效的复方苯甲酸散定量质控方法。方法:联合应用旋光法、化学容量法和吸收度线性组合分光光度法等分析技术,不经分离直接测定制剂中5种组分的含量。结果:苯甲酸、呋喃西林、鞣酸、水杨酸和硼酸5种组分平均回收率分别为99.99％、10O.07％、99.96％、100.06％和100.74％。RSD分别为0.50％、0.33％、0.92％、0.53％和0.50％。结论:实验表明所建立的定量分析方法简便易行,重现性好,对复方苯甲酸散制剂中5种组分进行质量控制的结果令人满意,可作为一种常规分析方法。     

0.5%盐酸麻黄碱滴鼻液含量测定方法研究

目的　建立 0 .5 %盐酸麻黄碱滴鼻液的含量测定方法。方法　比较旋光度法 ,酸碱滴定法 ,两种测定 0 .5 %盐酸麻黄碱滴鼻液的方法 ,挑选一种最佳的含量测定方法。结果　旋光度法在 2 .5～ 2 0mg/mL之间线性关系良好 (r=0 .99998) ,平均回收率为 10 0 .8% ,RSD =0 .0 5 4 %。结论　旋光度法简便快速 ,结果可靠可用于测定 0 .5 %盐酸麻黄碱滴鼻液。