匹维溴铵磷脂复合物的制备及其理化性质研究

采用单因素试验考察了反应溶剂、投料比、浓度、反应温度和反应时间对匹维溴铵磷脂复合物形成的影响.所得优化制备工艺条件为:以无水乙醇为反应溶剂,匹维溴铵浓度为5 mg/ml,匹维溴铵-大豆磷脂投料比为1:5,60℃反应2h.对制得的复合物进行紫外扫描、差示扫描量热及X-射线衍射分析,并考察了其溶解性能.结果表明,复合物中药物以无定形形态存在,且该复合物提高了匹维溴铵的表观油水分配系数.     

86例颞叶癫痫患者的临床特征和脑电图研究

目的探讨颞叶癫痫（temporal lobe epilepsy,TLE）的临床特征和脑电图（electroencephalogram,EEG）的特点。方法对86例已确诊为TLE的患者均作EEG和视频脑电图（V ideo-EEG,VEEG）检查,并结合临床症状进行分析。结果 86例患者的EEG正常39例,异常47例。VEEG正常5例,异常81例。其中左侧颞叶痫样放电30例,右侧颞叶痫样放电27例,双侧颞叶痫样放电18例,6例过度换气诱发试验出现癫痫波。临床症状中以强直-阵挛发作占TLE最多;TLE患者的EEG癫痫波多分布于一侧或两侧颞区。结论颞区的癫痫波对诊断TLE有重要作用;VEEG对提高TLE的诊断和定位具有重要意义。     

法人治理结构体制下的现代职业化医院管理模式

我国正在进行以＂法人治理结构＂为核心的公立医院管理体制改革。构建与＂法人治理结构＂相一致的新型医院管理模式就成为目前我们亟待解决的课题。笔者经过对东西方理念与文化融合的研究,于2001年10月提出＂现代职业化医院管理模式＂后,经过近十年的实践印证与改进,总结提出＂法人治理结构体制下的医院管理模式——现代职业化医院管理模式＂。此模式的提出将有利于改变我国医院管理的＂行政化与官员化＂,实现医院管理的＂专业化与职业化＂,从而加速我国医院管理的现代化进程,实现＂解放生产力、发展生产力、优化生产力＂之目的。     

基于有效组分检测与理化表征相结合的当归补血汤制备工艺与质量控制研究

目的 优选当归补血汤的制备工艺,探索以理化表征参数作为汤剂制备工艺评价与质量控制手段的可行性。方法 采用正交试验设计,主要以有效组分阿魏酸、黄芪甲苷、黄芪皂苷、多糖的量为指标,全面考察当归补血汤的制备工艺,并对所得提取液进行流变学、表面化学、电化学等方面的表征,将表征参数与组分的量进行多项式回归分析。结果 优选的当归补血汤制备工艺条件为取处方药材加12倍量水,提取3次,每次1.0 h;理化表征显示,不同制备工艺条件所得汤剂理化参数差别较大,且渗透压与体系组分的量关系最为密切,表面张力、电导率次之,而pH值、黏度与体系组分的量关系不密切。结论 初步表明,表面张力、电导率和渗透压可作为当归补血汤制备工艺与质量控制的评价指标,通过提取液的理化表征来进行质量控制具有一定的可行性。     

单相抑郁与双相抑郁患者的临床特征比较

目的:探讨单相抑郁与双相抑郁的临床特征比较。方法:随机选取我院两年来诊断为单相抑郁和双相抑郁的精神科住院患者各40例,用自行设计的记录表收集两组病例的临床资料,并进行统计学分析。结果:两组疾病在住院年龄(t=2.13)、起病年龄(t=3.08)、文化程度(x2=10.05)、病前性格(x2=7.53)、有无共病(x2=7.53)等方面存在显著性差异(P<0.05)。结论:单相抑郁与双相抑郁可能属于情感性精神障碍的异源性亚型。     

临床护士心理健康状况与人格特征的关系

目的探讨临床护士的心理健康状况与人格特征的关系,为医院心理健康教育工作提供理论依据。方法采用90项症状自评量表(SCL-90)和卡特尔16种人格因素问卷(16PF)对185名临床护士进行测评和分析。结果①SCL-90问卷显示,临床护士的总分和各因子分(除人际关系敏感外)高于全国常模;②与一般女性相比,临床护士在12个因子上存在显著差异;与一般护士相比,优秀护士具有高自律性、高怀疑性的人格特征;③稳定性、自律性与SCL-90得分呈显著负相关,怀疑性、忧虑性、紧张性与SCL-90得分呈显著正相关。结论临床护士的心理健康水平低于一般人群,其心理健康水平与人格特征之间关系密切。     

冬虫夏草鉴别方法的研究进展

冬虫夏草是麦角菌科真菌冬虫夏草菌Cordyceps sinensis (Berk.) Sacc.寄生在蝙蝠娥科昆虫幼虫上的子座及幼虫尸体的复合体.作为一味传统的名贵中药,冬虫夏草具有调节免疫系统功能、抗肿瘤、抗疲劳等多种药理作用.冬虫夏草的掺伪现象严重,综述冬虫夏草的鉴别方法,为快速、准确地对其进行鉴别提供参考.     

Comparison of biological characteristics of marrow mesenchymal stem cells in hepatitis B patients and normal adults

AIM: To establish a culture system of marrow mesenchymal stem cells (MSCs)from hepatitis B patients and normal adults and to compare their biological characteristics. METHODS: MSCs were isolated from bone marrow in 34 male hepatitis B patients and 15 male normal adults and cultivated in vitro. Their biological characteristics including surface markers, shapes and appearances, growth curves, first passage time and passage generations were compared. RESULTS: Cultivation achievement ratio of hepatitis B patients was lower than that of normal adults, no statistical significance (82.35% vs 100%, P > 0.05). Compared with MSCs of normal adults, MSCs of hepatitis B patients presented a statistical lower growth curve, longer first passage time (13.0 ± 1.6 d vs 11.4 ± 1.5 d, P < 0.05), fewer passaging generation numbers (10.5 ± 1.4 generations vs 12.3 ± 1.7 generations, P < 0.05), though both shared same appearances, shapes and surface markers. MSCs in hepatitis B patients would expand, spread out and age more easily and there were more refractive particles in the cytoplasm. CONCLUSION: MSCs from hepatitis B patients can be cultured in vitro. Although their appearance, shape and surface marker are similar to those of MSCs from normal adults, there are differences in their biological characteristics.     

过量表达铁蛋白基因的转基因烟草抗Co2＋能力分析

目的：将烟草铁蛋白基因NtFer1的完整cDNA序列克隆到植物表达载体pBI121中,利用农杆菌（Agrobactri-um tumefaciens）介导的叶盘法将NtFer1导入烟草（Nicotiana tobacum L.）基因组。对卡那抗性植株进行PCR-Southern检测,并将进一步经Northern杂交证明NtFer1基因表达量增加的转基因株系进行抗Co2＋分析。结果表明,NtFer1的过量表达提高了转基因植株的抗Co2＋能力。在含150μmol·L-1Co2＋的MS培养基上,转基因植株的株高和鲜重均明显优于非转基因株系;在生理性状上表现为叶绿素含量、POD活性和SOD活性比非转基因株系明显增加,转基因植株MDA含量明显低于非转基因株系。结果表明,NtFer1大量表达能够增强转基因株系抗氧化能力,提高植物抗Co2＋能力。     

Preparation,physicochemical characterization and biological evaluation of cefodizime metal ion complexes

Objectives Cefodizime is a broad spectrum cephalosporin belonging to the third generation agents. In this study, attention has been paid to the preparation, physicochemical characterization and biological evaluation of new Cu²⁺, Zn²⁺, Fe³⁺, Co²⁺ and Al³⁺ complexes of cefodizime. Methods The stoichiometrics and the mode of bonding of the complexes were deduced from their elemental and metal analysis, electrical conductivity measurements, UV‐vis, infrared and Raman spectroscopic investigations. Study of the stoichiometry of these complexes referred to the formation of 1: 1 ratios of metal to ligand. Antimicrobial activity of the complexes was determined using two strains of Gram‐positive (Bacillus subtilis and Proteus vulgaris) and two strains of Gram‐negative (Escherichia coli W3110 and Pseudomonas putida) bacteria. The minimal inhibitory concentration was determined as the lowest concentration inhibiting bacterial growth on solid Luria Bertani medium. Key findings The spectra gave evidence as to the position of binding. In addition, the aqueous solubility of cefodizime was strongly reduced by complexation. Conclusions The antibacterial activity of cefodizime was not affected by complexation with Al³⁺ but it was reduced by complexation with the other tested metal ions against the bacteria under study.