全文获取类型
收费全文 | 164944篇 |
免费 | 15819篇 |
国内免费 | 5274篇 |
专业分类
耳鼻咽喉 | 1180篇 |
儿科学 | 2268篇 |
妇产科学 | 1945篇 |
基础医学 | 28033篇 |
口腔科学 | 4000篇 |
临床医学 | 11584篇 |
内科学 | 22003篇 |
皮肤病学 | 3148篇 |
神经病学 | 13912篇 |
特种医学 | 4184篇 |
外国民族医学 | 45篇 |
外科学 | 12873篇 |
综合类 | 20354篇 |
现状与发展 | 23篇 |
预防医学 | 8389篇 |
眼科学 | 1593篇 |
药学 | 24309篇 |
38篇 | |
中国医学 | 6878篇 |
肿瘤学 | 19278篇 |
出版年
2024年 | 251篇 |
2023年 | 2240篇 |
2022年 | 3408篇 |
2021年 | 6526篇 |
2020年 | 5377篇 |
2019年 | 7807篇 |
2018年 | 7234篇 |
2017年 | 6288篇 |
2016年 | 5527篇 |
2015年 | 6790篇 |
2014年 | 10263篇 |
2013年 | 10060篇 |
2012年 | 8817篇 |
2011年 | 10304篇 |
2010年 | 8715篇 |
2009年 | 9355篇 |
2008年 | 8464篇 |
2007年 | 8507篇 |
2006年 | 7479篇 |
2005年 | 6598篇 |
2004年 | 5043篇 |
2003年 | 4809篇 |
2002年 | 3816篇 |
2001年 | 3360篇 |
2000年 | 2896篇 |
1999年 | 2493篇 |
1998年 | 2331篇 |
1997年 | 2136篇 |
1996年 | 1917篇 |
1995年 | 1704篇 |
1994年 | 1459篇 |
1993年 | 1273篇 |
1992年 | 1012篇 |
1991年 | 919篇 |
1990年 | 726篇 |
1989年 | 631篇 |
1988年 | 616篇 |
1987年 | 614篇 |
1986年 | 723篇 |
1985年 | 1180篇 |
1984年 | 1172篇 |
1983年 | 848篇 |
1982年 | 868篇 |
1981年 | 718篇 |
1980年 | 696篇 |
1979年 | 523篇 |
1978年 | 325篇 |
1977年 | 292篇 |
1976年 | 282篇 |
1975年 | 192篇 |
排序方式: 共有10000条查询结果,搜索用时 25 毫秒
91.
92.
93.
94.
95.
Extensive research has indicated that miRNAs are crucial for the occurrence and progression of cancers. miR-451a, involved in breast cancer (BC), is one of the miRNAs. This study focused on the mechanism by which miR-451a regulates BC. The levels of miR-451a in BC tissues and cell lines were examined using quantitative real-time polymerase chain reaction (qRT-PCR). Kaplan‒Meier analysis showed that this was intimately related to the patient's overall survival rate. Functional experiments revealed the negative effects of miR-451a on the abilities of BC cells to multiply (tested by Cell Counting Kit-8), migrate (tested by wound healing assay), and invade (tested by Transwell assay) and its positive effects on apoptosis (tested by flow cytometry). Western blotting indicated that the expression of tumor-related proteins was affected by miR-451a. Moreover, in vivo experiments suggested that tumor growth was clearly restrained by an miR-451a agonist in a xenograft tumor model. Bioinformatic analysis indicated that miR-451a directly targeted Cyclin D2 (CCND2), as demonstrated by the luciferase reporter assay. An opposite change in the level of CCND2 and miR-451a in BC was indicated by qRT-PCR, western blotting, and immunohistochemistry. Subsequently, functional experiments and western blotting analysis confirmed that CCND2 accelerated BC progression, which was regulated by miR-451a. Cumulatively, research on miR-451a may be valuable for BC treatment. 相似文献
96.
97.
98.
目的:比较胶质瘤患者胶质瘤组织和瘤旁组织中miR-147表达水平的差异性,进一步分析miR-147与胶质瘤细胞生物学活性的相关性。方法:通过实时荧光定量PCR检测65例胶质瘤患者瘤组织和瘤旁组织中miR-147的表达水平;采用Lipofectamine 2000转染法转染胶质瘤细胞,分为miR-147 mimics组和NC组,检测两组转染效率;进一步通过CCK-8实验、流式细胞实验和Transwell实验比较两组细胞生物活性。结果:胶质瘤组织中miR-147表达水平显著低于瘤旁组织;与NC组比较,miR-147 mimics组细胞不论是增殖能力还是侵袭能力都明显降低,但是,miR-147 mimics组的细胞凋亡率升高。结论:胶质瘤组织中存在miR-147低表达的现象。miR-147在胶质瘤细胞中表达升高能够抑制细胞的增殖能力、降低其侵袭能力,并促使更多的细胞发生晚期凋亡。miR-147可能在胶质瘤中扮演着抑癌基因的重要角色。 相似文献
99.
100.
《European journal of medical genetics》2020,63(6):103919
Neuronal growth regulator 1 (NEGR1), a member of the immunoglobulin superfamily cell adhesion molecule subgroup IgLON, has been involved in neuronal growth and connectivity. Genetic variants, in or near the NEGR1 locus, have been associated with obesity and, more recently, with learning difficulties, intellectual disability, and psychiatric disorders.Here, we described the only second report of NEGR1 gene disruption in 1p31.1 microdeletion in two patients. Patient 1 is a 14-year-old female with neurological and psychiatric features present also in her family. Patient 2 is a 5-month-old infant showing global hypotonia as unique neurological features till now. This patient also carries 7p22.1 duplication, of paternal origin, that could be responsible for some malformations present in the child.We hypothesize a role of NEGR1 in producing the phenotype of our patients and compare them with other cases previously reported in the literature and DECIPHER database to better identify a possible genotype-phenotype correlation. 相似文献