载骨髓间充质干细胞的磷酸钙骨水泥的制备

[目的]观察载骨髓间充质干细胞(Bone marrow mesenchymal stem cells,MSCs)明胶微球磷酸钙骨水泥(Calcium phosphate cement,CPC)的理化性质,探究新型CPC的制备。[方法]制备载MSCs的明胶微球后,分别以0%(A),2.5%(B),5%(C),10%(D)的质量比(w/w)与CPC复合。测定其抗压强度、初凝时间、孔隙率和大孔率等理化性质,进一步检测MSCs在CPC中的成长情况。[结果]在各组CPC中MSCs均生长良好,且与空白对照组比较,随着明胶微球比例的增大,各组CPC的抗压强度逐步降低,初凝时间延长,孔隙率增大,大孔率增高(P<0.05)。[结论]载MSCs的明胶微球与CPC的质量比为5%时,CPC的理化性质最为理想,细胞生长良好,此结果为下一步体内实验奠定了坚实的基础。     

Genipin crosslinked curcumin loaded chitosan/montmorillonite K-10 (MMT) nanoparticles for controlled drug delivery applications

Abstract

Here, we have reported the influence of MMT and genipin in releasing curcumin from the Genipin crosslinked Chitosan/MMT nanoparticles, prepared by ionic gelation method. The nanoparticles were characterised using Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffractometry (XRD), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM). Zeta potential and average diameter of the nanoparticles were found in the range 32–47?mV and 430–560?nm. Swelling and release of curcumin from the nanoparticles increased with the decrease in pH of the medium, MMT, and genipin content. Curcumin released from the nanoparticles reduced the viability of MCF-7 and Hep G2 cells as compared to untreated cells. The nanoparticles increased the level of reduced glutathione (GSH), superoxide dismutase (SOD), and catalase level in human PBMCs and decreased the level of Lipid peroxidation suggesting an enhanced protection against cellular damage. Lower pH and higher MMT concentration in the nanoparticles improved the mucoadhesive properties.     

Controlled Release of DSBP from Genipin-Crosslinked Gelatin Thin Films

Controlled release of a marker drug, 4,4′-bis(2-sulfostyryl) biphenyl (DSBP) from genipin crosslinked gelatin thin films, with application to drug delivery by transdermal patches is studied in this paper. A simple method for fabrication of nano-thin films, using basic lab equipment is introduced. This method consists of two steps: dipping of the substrate in a deposition solution, followed by centrifugation of the substrate. Also, swelling and drug release from thin films is modeled, using the Fick's second law of diffusion. The effect of genipin concentration on release of DSBP molecules from thin films is investigated, experimentally and numerically. The results show that controlled release of DSBP from the genipin-crosslinked gelatin thin films is achieved, using various concentrations of genipin in gelatin.     

Genipin对糖尿病小鼠下丘脑细胞凋亡指数及解偶联蛋白2表达的影响

目的探讨genipin对糖尿病小鼠下丘脑细胞凋亡指数(AI)及解偶联蛋白2(UCP2)表达的影响。方法 48只健康雄性小鼠随机分为正常对照组、糖尿病模型组和genipin低、高剂量干预组,每组12只小鼠。采用长期高脂饮食及链脲佐菌素胰岛破坏法建立2型糖尿病小鼠模型。Genipin低、高剂量干预组小鼠分别给予5 mg/(kg.d)、10 mg/(kg.d)的genipin 0.1 ml连续灌胃2周,正常对照组和糖尿病模型组用等量生理盐水替代。分别采用TUNEL染色法和免疫组化染色检测并计算各组小鼠下丘脑AI和UCP2蛋白阳性细胞率;采用实时PCR和Western Blot法检测下丘脑UCP2 mRNA和蛋白的表达水平。结果糖尿病模型组、genipin低剂量和高剂量干预组下丘脑AI明显高于正常对照组(均P<0.05);而genipin低剂量和高剂量干预组下丘脑AI明显低于糖尿病模型组(均P<0.05);且genipin低剂量干预组和genipin高剂量干预组间差异无统计学意义。糖尿病模型组UCP2蛋白阳性细胞率明显高于正常对照组(P<0.05),而genipin低剂量和高剂量干预组UCP2蛋白阳性细胞率均明显低于糖尿病模型组(均P<0.05)。Genipin低剂量和高剂量干预组UCP2 mRNA和蛋白的表达水平均明显低于糖尿病模型组(均P<0.05)。与genipin低剂量干预组比较,geni-pin高剂量干预组UCP2蛋白阳性细胞率、UCP2 mRNA和蛋白的表达水平明显降低(均P<0.05)。结论 Ge-nipin可以减少糖尿病小鼠下丘脑细胞凋亡和UCP2的表达。     

Rapid treatment of full‐thickness skin loss using ovine tendon collagen type I scaffold with skin cells

The full‐thickness skin wound is a common skin complication affecting millions of people worldwide. Delayed treatment of this condition causes the loss of skin function and integrity that could lead to the development of chronic wounds or even death. This study was aimed to develop a rapid wound treatment modality using ovine tendon collagen type I (OTC‐I) bio‐scaffold with or without noncultured skin cells. Genipin (GNP) and carbodiimide (EDC) were used to cross‐link OTC‐I scaffold to improve the mechanical strength of the bio‐scaffold. The physicochemical, biomechanical, biodegradation, biocompatibility, and immunogenicity properties of OTC‐I scaffolds were investigated. The efficacy of this treatment approach was evaluated in an in vivo skin wound model. The results demonstrated that GNP cross‐linked OTC‐I scaffold (OTC‐I_GNP) had better physicochemical and mechanical properties compared with EDC cross‐linked OTC‐I scaffold (OTC‐I_EDC) and noncross‐link OTC‐I scaffold (OTC‐I_NC). OTC‐I_GNP and OTC‐I_NC demonstrated no toxic effect on cells as it promoted higher cell attachment and proliferation of both primary human epidermal keratinocytes and human dermal fibroblasts compared with OTC‐I_EDC. Both OTC‐I_GNP and OTC‐I_NC exhibited spontaneous formation of bilayer structure in vitro. Immunogenic evaluation of OTC‐I scaffolds, in vitro and in vivo, revealed no sign of immune response. Finally, implantation of OTC‐I_NC and OTC‐I_GNP scaffolds with noncultured skin cells demonstrated enhanced healing with superior skin maturity and microstructure features, resembling native skin in contrast to other treatment (without noncultured skin cells) and control group. The findings of this study, therefore, suggested that both OTC‐I scaffolds with noncultured skin cells could be promising for the rapid treatment of full‐thickness skin wound.     

Development of HPLC Method to Evaluate Drug-processing Technique of Eucommiae Cortex

Objective To establish a RP-HPLC method investigate the processing technique and mechanism of Eucommiae Cortex.Methods The RP-HPLC method was applied to simultaneously determining six ingredients,geniposidic acid,geniposide,genipin,chlorogenic acid,(+)-pinoresinol-di-β-D-glucopyranoside,and(+)-syringaresinol-di-β-D-glucopyranoside,in the different processed barks of Eucommia ulmoides.Results The valid method with good accuracy could be well used to study the processing technique of E.ulmoides;Besides,target ingredients in E.ulmoide were decreased within 6 h when they were processed.Conclusion Established RP-HPLC is a reliable method which could be used to research the processing technique of the barks of E.ulmoides.Moreover,the result of this study could be provided with significant evidence of processed barks of E.ulmoides.     

Study on Characteristics of Acellular Bovine Pericardium Crosslinked with Genipin

The study used a naturally occurring crosslinking reagent-genipin to chemically modify acellular bovine pericardium, prepare cardiac valve tissue engineering scaffold material,and evaluated genipin crosslinked acellular matrix of bovine pericardium by investigating the physical and chemical properties of the tissues, such as the surface properties, crosslinking characteristics, mechanical properties, resistance to enzymatic capacity in vitro, and hemolysis tests. The results showed that acellular bovine pericardium matrix crosslinked with genipin was strong hydrophilicity, high crosslinking index, and stable structure, which can maintain good mechanical properties. As a kind of scaffold material for valve tissue engineering, it has wide application prospect.     

A pilot study of genipin cross-linking effects on bullous keratopathy in rabbits

目的:评价京尼平交联治疗兔大泡性角膜病变的效果.方法:将已建立大泡性角膜病变动物模型的9只雌性新西兰白兔随机分为3组:治疗组(n=3),对照组(n=3),空白对照组(n=3).其中治疗组:刮除角膜上皮后,应用0.25%的京尼平浸泡实验眼角膜40min(24℃);对照组:刮除角膜上皮后,应用生理盐水浸泡实验眼角膜40min(24℃);空白对照组:不进行任何处理.处理后2wk内进行以下检查:裂隙灯检查、中央角膜厚度(CCT)测量、体质量和应激反应评估、组织学检查和利用原位末端转移酶标记技术(TUNEL法)检测基质层细胞凋亡.结果:与对照组和空白对照组相比,治疗组角膜水肿明显减轻、角膜上皮逐渐修复完整、角膜大泡消失、中央角膜角膜厚度显著下降(P<0.05)、体质量显著增加(P<0.05).治疗组的实验动物活动性较处理前增加,反抗行为减少,攻击性减低.治疗组角膜基质层胶原纤维排列更为紧致、规则,可见蓝色条索状交联产物.治疗组角膜基质层均偶见凋亡细胞,对照组及空白对照组未明显见凋亡细胞.结论:京尼平交联治疗可使兔大泡性角膜病变角膜水肿减轻,疼痛症状缓解,其可能的机制为京尼平交联使角膜基质层胶原纤维排列紧密,从而阻止角膜水肿和大泡的形成.     

Chemistry and bioactivity of Gardenia jasminoides

Gardenia jasminoides, grown in multiple regions in China, was commonly used as a natural yellow dye but has been one of the popular traditional Chinese medicines since the discovery of its biological property a few decades ago. It has been reported that G. jasminoides possess multiple biological activities, such as antioxidant properties, hypoglycemic effect, inhibition of inflammation, antidepression activity, and improved sleeping quality. In this review, our aim was to have a comprehensive summary of its phytochemistry including the extraction, isolation, and characterization of volatiles and bioactive molecules in G. jasminoides, focusing on the two major phytochemicals, genipin and crocin, which possess potent medicinal properties. Furthermore, this study attempted to establish a structure–activity relationship between the two major series of molecules with two pharmcophores and their biological activities, which would serve further exploration of the properties of phytocompounds in G. jasminoides as potential functional foods and medicines.