H—TdR前体掺入技术在复方中药抗癌制剂研究中的应用

将一种快速、灵敏、可靠、简便的抗癌活性微量测定技术——~3H—TdR前体掺入技术用于复方中药抗癌制剂制备工艺筛选,短期内即拟定出较合理的工艺,经临床及14项生物学指标综合评定,该制剂疗效好,无明显的毒副作用。表明把一些快速、灵敏的医学检测技术引入药物制备工艺、质量标准及稳定性研究,可克服由于活性成分不清楚带来的一些困难,拓宽了研究途径,提高了研究水平。     

Leukocytosis and low serum IgA in workers exposed to the epoxy compound,t-methyl-3-phenylglycidate

Summary Forty-nine out of 54 male workers engaged in the production of an epoxy compound, t-methyl-3-phenylglycidate, showed skin symptoms in varing degrees that may be due to the skin-irritative effect of the compound. The exposed workers were also shown to have subjective symptoms which may be related to the irritative property of the compound on surface tissue. Laboratory examinations on the blood obtained from the exposed workers showed significantly higher values of leukocyte concentration as compared with the non-exposed controls. This was chiefly caused by the increase of neutrophilic granulocytes and T-cell lymphocytes. Serum IgA levels of the exposed workers were shown to be significantly lower than those of the control group. Hemoglobin concentration, hematocrit value and red cell count of the exposed workers remained at the same level as those of the control subjects. Liver or kidney damage was not found in biochemical analyses on the sera of exposed workers.     

丹参抗大鼠乙酸性十二指肠溃疡作用机制的探讨

目的　探讨丹参抗大鼠乙酸性十二指肠溃疡的作用及机制。方法　按Okabe方法复制大鼠乙酸十二指肠溃疡模型 ,按传统方法测定溃疡指数、溃疡抑制率 ;用放射免疫法测定十二指肠溃疡部位的前列腺环素 (PGI2 )含量。结果　丹参组的溃疡指数显著低于对照组 (P <0 .0 1) ,其溃疡抑制率高于对照组 ,PGI2 的含量亦显著高于对照组 (P <0 .0 1)。结论　丹参具有抗大鼠十二指肠溃疡的作用 ,其机制可能是丹参提高了十二指肠粘膜的防御能力     

复方丹参滴丸对急慢性高粘滞血症模型血管内皮细胞分泌功能的影响

目的：观察复方丹参滴丸对高粘滞血症血管内皮分泌功能的影响，探索其作用机制。方法：复合因素(高分子右旋糖酐、肾上腺素、牛血清白蛋白)、长时间(112天)造成高粘滞血症慢性模型；一次性静脉注射高分子右旋糖酐。皮下注射(sc)肾上腺素造成急性高粘滞血症模型，分别用复方丹参滴丸进行治疗，观察血管肉皮细胞分泌功能的变化。结果：慢性高粘滞血症模型血浆血栓素B2(TXB2)和内皮素(ET)浓度非常显升高，而6-酮-前列腺素F1x(6-酮)浓度非常显降低；急性高粘滞血症模型血管内皮分泌功能无显变化；长期使用复方丹参滴丸能调整、改善血管内皮分泌功能的异常状态；复方丹参滴丸改善血管内皮分泌功能的即时效应不显。     

药用真空铝塑复合薄膜的质量试验

 根据文献对药用真空铝塑复合薄膜进行了理化性能、密闭性能、急性毒性试验。结果表明，目前国产药用真空铝塑复合薄膜的铅盐、高锰酸钾还原性物质，蒸发残渣、密闭性能均不合格，但急性毒性试验符合规定。建议改进生产，提高质量。     

盐酸氯胺酮-β-环糊精包合物的研究

采用正交设计法对饱和水溶液法制备盐酸氯胺酮－β－环糊精包合物的温度、时间及投料比进行选优，显微观察包合物形态，UV和X－射线衍射法测试包合物晶形变化，品尝包合物味觉效果，并测定其稳定性及溶出度。结果表明，按1：2主客克分子比，于25℃电动搅拌3h，制备包合物的包合率和收率较高，包合物已形成一种新物相，具有稳定性高，无苦味及缓释性能。     

装潢居室空气中挥发性化学物的遗传毒性研究

目的探讨装潢居室空气中挥发性化合物的遗传毒性.方法采用小鼠骨髓嗜多染性红细胞微核试验和小鼠精子畸形试验检测居室装潢后空气中挥发性化合物的遗传毒性.结果该化合物对小鼠呼吸系统有明显的刺激作用,与阴性对照组比较,高、中、低剂量组的微核率、精子畸形率均有显著差异性.结论居室装潢后室内空气中挥发性化合物具有遗传毒性作用.     

Oxygen Tension Imaging in the Mouse Retina

A newly developed microscope-based imaging system was used to measure the oxygen tension (PO₂) inside the retinal and choroidal vessels of mice and to generate in vivo maps of retinal PO₂. These maps were generated from the phosphorescence lifetimes of an injected palladium–porphyrin compound using a frequency-domain measurement. The system was fully calibrated and used to produce retinal PO₂ maps at different inspiratory oxygen fractions. PO₂ rose accordingly and predictably as inspiratory O₂ was stepped from hypoxic to hyperoxic conditions. Important experimental and acquisition parameters necessary for applying phosphorescence lifetime imaging to the mouse eye were investigated, including camera exposure and intensifier gain settings. Because of a need to limit light exposure to the retina, PO₂ map quality as measured by the coefficient of determination was investigated as a function of signal-to-noise and accumulated excitation energy deposition. With the development of this technology for use in mice, the potential for investigating the oxygen dynamics in genetically engineered mouse models of retinal disease, including diabetic retinopathy, glaucoma, and age-related macular degeneration, is advanced. © 2003 Biomedical Engineering Society. PAC2003: 4266Ew, 8763Lk, 8719Dd     

Effects of local nerve cooling on conduction in vagal fibres shed light upon respiratory reflexes in the rabbit

In ten vagus nerves the effect of local cooling on the compound action potential was studied in the temperature range of 34 to 0 °C in spontaneously breathing, anaesthetized rabbits. The mean temperature at which the myelinated (A) fibres were completely blocked, was 10.2±2.4 °C (mean ± S.D.). In nine nerves, local vagus cooling to 0 °C failed to block all non-myelinated (C) fibres. In one nerve, total blocking occurred at 2.0 °C. We conclude that in the rabbit, the earlier found increase in tonic activity of the diaphragm following lung inflation or deflation during bilateral local vagus cooling to a temperature between 8 and 0 °C is due to afferent impulses in vagal C fibres.     

前臂后皮神经营养血管远端蒂复合瓣的应用解剖

目的:为前臂后皮神经营养血管远端蒂复合瓣设计提供解剖学基础。方法:30侧动脉灌注红色乳胶成人上肢标本,解剖观测前臂后皮神经营养血管的来源、分支、吻合及其与尺、桡骨膜血管的关系。结果:前臂后皮神经营养血管来自:桡侧副动脉皮支2～6支,外径(0.6±0.3) mm;骨间后动脉皮支6～9支,外径(0.7±0.3) mm;骨间前动脉腕背支皮支3~5支,外径(0.8±0.2) mm;尺、桡动脉腕背支皮支2~6支,外径(0.6±0.1) mm。尺骨中上段骨膜血管来自骨间后动脉的肌骨膜支6～8支,外径0.3～1.0 mm;骨间后动脉桡侧骨皮支与桡骨中段裸区骨膜血管吻合。上述支发出皮支、筋膜支、骨膜支和神经营养血管,形成皮神经干血管链以及深、浅筋膜和骨膜血管网。结论:前臂后皮神经营养血管与肌、骨、皮营养血管同源,其远端蒂复合瓣,旋转轴点在腕关节平面,适宜手背远处的组织缺损修复。