保留冠状动脉的猪心螺旋形心室肌带的解剖

Objective Based on the Helical Ventricular Myocardial Band (HVMB) theory proposed by Torrent-Guasp,the ventricular myocardial hand extends from the root of the pulmonary artery to the root of the aorta with two helical coils.This new theory is considered as a revolutionary concept for further understanding the global, three-dimensional and functional architecture of the ven- tricular myocardium. No repot had described techniques for disecting HVMB while keepin～ the integrity of the coronmy artery sys- tern. We explored techniques for dissecting HVMB in swine.Methads 33 fresh swine hearts were randomly divided intoll groups, 3 bearts in each. 160% barium sulfate (type I)suspmmion was injected into the coronary artery system. The coronary arteries were li- gated. The strial tissue was removed following puuing the hearts in boiling water then cooling for several hours. The superficial coro- nary vessels and fat tissue around the atrio-ventricular taxi inter-ventricular sulcus we～'e preserved. Some branches of the left anterior descending artery, distal segment, of posterior descending branch, and middle and distal segment of obtuse marginal branches were mu- tilated appropriately. HVMB dissection was completed with fingers in accordnce with Torrent Guasp' s technique. Results A contin- ued bundle of muscle, originated at the root of pulmonary artery and ended at the root of aorta, was unwrapped along the major dire- tion of the cardiac muscle fiber in all of the 33 hearts with spating of the coronary artery. The swine hearts' ventricular myocandium was cumosed of two loops, with basal loop firm the root of the pulmonart artery to the anterior papillary muscle and apical from the beginning of the anterior papillary muscle to the root tithe aorta. Each loop consisted of two segments: the right segment-coincid- ing with the right ventricular free wall and the left segment-coinciding with the basal d the left ventricular free wall. Posterior papillary muscle, which belongs to the descendant segment, denmrcated the border between the descendent and the ascendant of the HVMB's apical loop. Conclusion Although controversies about the theory of the HVMB remain, we have dissected the HVMB in the swine hearts' ventricular myocardium successfully with sparing of the coronary artery systems. This dissection procedure provides technical information for the studies of associated diseases based on the theory of HVMB.     

降糖益肾方合二甲双瓜治疗2型糖尿病50例

目的:观察中药降糖益肾方合二甲双胍片治疗2型糖尿病的临床疗效.方法:治疗组50例口服降糖益肾方、二甲双胍片.对照组45例口服二甲双胍片.两组均连续用药3个月,监测两组治疗前后空腹及餐后2h血糖(FPG、PG2h)、空腹胰岛素(Fins)血脂(TC、TG)及血液流变学指标变化,并计算胰岛素敏感指数(IAI).结果:治疗组临床症状改善、FPG、PG2h、Fins、TC、TG以及血浆粘比度、红细胞压积、纤维蛋白原与对照组比较明显降低,IAI明显提高(P＜0.01、P＜0.05).结论:中药降糖益肾方合二甲双胍片治疗2型糖尿病疗效确切.     

硫酸镁和川芎嗪对新生鼠缺氧缺血性脑损害的预防作用

建立新生鼠缺氧缺血性脑病（HIE）的模型，观察了大脑皮质超氧化物歧化酶（SOD）、丙二醛（MDA）、血清MDA和脑组织神经病理变化以及硫酸镁、川芎嗪对其影响。发现HIE组大脑皮质SOD、MDA和血清MDA升高明显，神经细胞变性显著。硫酸镁和川芎嗪能使大脑皮质SOD、MDA和血清MDA降低，神经细胞变性减轻。提示氧自由基在缺氧缺血性脑损害发病中的影响和硫酸镁、川芎嗪通过间接抗氧化作用对HIE的预防作用。     

硫酸铁铵催化合成乙酰乙酸乙酯乙二醇缩酮

采用正交试验优选出最佳条件为：当乙酰乙酸乙酯为0.2moL时，硫酸铁铵0.7g，环乙烷10mL。乙酰乙酸乙酯和惭二醇摩尔比为1：1.5，反应时间为5h。所得产率为59.2%。     

妇科恶性肿瘤术后骨质疏松症的防治

为探讨妇科恶性肿瘤术后骨质疏松症的预防措施,采用补充紫竹爱维和乐力胶囊来预防骨质疏松的发生,分别与未服药的妇科恶性肿瘤及良性肿瘤术后作对照,并对其对骨代谢指标血清骨性碱性磷酸酶(BALP)及血清抗酒石酸酸性磷酸酶(TRAP)的影响进行了临床观察.结果发现恶性肿瘤术后较良性肿瘤术后患者的BALP降低,TRAP增高,服用紫竹爱维与乐力胶囊的患者TRAP均低于未服药的恶性肿瘤术后患者,P＜0.05,均有显著性差异.表明妇科恶性肿瘤术后患者较良性肿瘤更易患骨质疏松症,服用紫竹爱维与乐力胶囊均能有效防治骨质疏松症,前者效果更佳,但应掌握性激素补充治疗(HRT)的应用指征.     

The in vitro elution characteristics of vancomycin and tobramycin from calcium sulfate beads

The purpose of this study was to determine the elution characteristics of vancomycin and tobramycin when mixed with calcium sulfate to form antibiotic beads. Calcium sulfate was combined with vancomycin and tobramycin separately to form 2 types of antibiotic beads, which were packaged and labeled separately. The packaged calcium sulfate beads with vancomycin and tobramycin were then gas sterilized. The beads were placed in phosphate-buffered saline and kept at 36 degrees C for 6 weeks. Two separate series of assays were run simultaneously for both types of beads. In one assay, a bead containing vancomycin was placed in a fresh vial of phosphate buffered saline after each assay. The same was done with beads containing tobramycin. In the second series of assays, 9 vials of phosphate buffered saline each containing 1 vancomycin bead and 9 vials of phosphate buffered saline each containing 1 tobramycin bead was arranged. The phosphate-buffered saline was then assayed at predetermined times for both the vancomycin bead series and the tobramycin bead series. The amount of vancomycin and tobramycin assayed nearly equaled the calculated amount of antibiotic per bead measured before bead construction. Also, the elution of antibiotic from the calcium sulfate was complete within 72 hours. In conclusion, the construction and gas sterilization of calcium sulfate beads containing vancomycin and tobramycin does not destroy vancomycin and tobramycin. Also, the complete elution of available vancomycin and tobramycin in calcium sulfate beads occurs within 72 hours.     

人急性胰腺炎早期外周血单核细胞Toll样受体4及CD14表达变化

目的探讨人急性胰腺炎早期外周血单核细胞（peripheral blood monocytes，PBMCs）表面Tbll样受体4（toll like receptor4，TLR4）、CD14表达变化。方法收集发病24h内入院的早期急性胰腺炎病人36例，采集入院当日及第3、第7日外周血，分离单核细胞。用流式细胞术检测单核细胞表面TLR4、CD14表达变化情况。同时检测血清肿瘤坏死因子（tumor necrosis factor alpha，TNF-α）、白细胞介素6（interleukin6，IL-6），研究它们之间的相关性。结果急性胰腺炎病人外周血单核细胞表面TLR4发病后表达上调，在轻症病人渐下降，1周左右恢复正常。TNF-α的变化一致。结论人急性胰腺炎早期可能通过先天性免疫门户蛋白TLR4激活单核巨噬细胞系统导致TNF-α等促炎细胞因子的产生和释放。     

猪喉软骨硫酸软骨素的制备和自由基清除活性

直接用木瓜蛋白酶水解软骨,三氯醋酸除蛋白质,乙醇沉淀干燥得硫酸软骨素粗多糖,经DEAE-Sepharose fast flow离子交换层析分离纯化,高效凝胶渗透色谱和琼脂糖凝胶电泳测定其相对分子质量和纯度,比较硫酸软骨素粗品和纯品的体外自由基清除活性。结果显示硫酸软骨素粗品和纯品提取率分别为31.56±0.46%和19.79±0.78%,后者相对相对分子质量为75 174,粗品的DPPH.和.OH清除活性最强,随着产品纯度的提高,活性降低,而对于O2-.清除活性结果则相反。     

非洛地平缓释片在兔体内药物动力学和生物利用度比较

目的 比较几种国产非洛地平缓释片在兔体内的药物动力学和生物利用度.方法 用高效液相色谱法测定兔口服药物后血浆中的药物浓度,以普通非洛地平片作为参照,计算其药物动力学和生物利用度.结果 在1.0-80μg·L-1范围内,非洛地平血浆药物浓度与色谱峰高呈良好的线性关系.单剂量兔血浆中非洛地平缓释片动力学参数不同产品存在差异,相对于普通制剂的生物利用度分别为179%、162%、122%.结论 不同产品非洛地平缓释片药物动力学和生物利用度存在差别.     

盐酸吗啡普通片及硫酸吗啡控释片在不同溶剂中溶释过程的对照研究

本文采用紫外分光光度法对国产盐酸吗啡普通片及合资厂产硫酸吗啡控释片于蒸馏水、人工胃液、人工肠液中进行体外溶出度和释放度测定。结果表明，两种片剂的溶释均符合中国药典９５版标准，控释片在人工肠液中溶释参数Ｔ５０、Ｔｄ、ｍ与在蒸馏水及人工胃液中的溶释参数差异具显著性（Ｐ＜０．０１），在人工肠液中的释放过程明显缓慢。