儿童益智口服液中海参水解工艺改进研究

目的　研究并改进儿童益智口服液中海参水解工艺。方法　采用 5因素 4水平L16(45)正交试验法 ,以水解度为考察指标 ,对影响水解的因素进行研究。结果　影响海参水解的主次因素为B >A >D >C。优选的水解工艺条件为 :加酶量 (A)1.5 %、加水量 (B) 2 0倍、水解时间 (C) 6h、水解温度 (D) 5 0℃。按此优选工艺生产 ,水解度达 (2 9.2± 0 .9) % (n =3) ,与原工艺所得水解度经t检验比较 ,有极显著差异 (P <0 .0 0 1)。结论　上述结果为儿童益智口服液中海参水解工艺的改进提供了依据     

花刺参酸性粘多糖的提取及鉴定

利用 2 %NaOH和胃蛋白酶由花刺参中提取酸性粘多糖,酸性粘多糖纯度可达 95 %,提取方法简单可行,提取率高     

海参活素抗疲劳作用的初步研究

目的研究海参活素的抗疲劳作用。方法采用动物抗疲劳作用实验方法 ,设低、中、高 3个剂量组30 0、6 0 0、1 2 0 0mg·kg·d- 1,另设空白对照组 (生理盐水 ) ,按剂量设计连续给小鼠受试物 1 5d。结果低剂量海参活素可明显延长小鼠爬杆时间 ,降低小鼠运动后血清尿素氮和血乳酸含量 ,升高血糖和肝糖原含量。结论初步研究表明 ,海参活素具有一定的抗疲劳作用     

A new membrane-attack complex/perforin (MACPF) domain lethal toxin from the nematocyst venom of the Okinawan sea anemone Actineria villosa.

The Okinawan sea anemone Actineria villosa causes severe cases of stinging. We isolated the 60 kDa A. villosa toxin (AvTX-60A) as the major toxin from the isolated nematocysts of this species. AvTX-60A showed fatal toxicity to mice with intraperitoneal injection at a minimum lethal dose of less than 250 microg/kg. The N-terminal amino acid sequence was determined and the corresponding cDNA encoding AvTX-60A was sequenced. The deduced amino acid sequence of AvTX-60A showed high similarity with PsTX-60A, which had been isolated as one of the major toxins from the venomous sea anemone Phyllodiscus semoni. These sea anemone toxins are new members of the family of proteins containing membrane-attack complex/perforin (MACPF) domains, best known in pore forming proteins such as perforin. These are the first examples of MACPF domain proteins as toxins for prey acquisition or repelling predators in nature.     

The potential health impacts of climate change: An overview

Climate change would have a range of impacts on human health. Health impacts would be caused by the direct effect of climatic factors on human health, such as heat stress, and possible changes in the frequency and intensity of extreme weather events such as storms, floods and droughts. Impacts on health would also be mediated by the indirect effects of climate change, such as changes in availability of food and water and the distribution of vector‐borne diseases. The majority of health impacts would be adverse and would depend greatly on the vulnerability of populations.     

Cell mediated immune response of the Mediterranean sea urchin Paracentrotus lividus after PAMPs stimulation

The Mediterranean sea urchin (Paracentrotus lividus) is of great ecological and economic importance for the European aquaculture. Yet, most of the studies regarding echinoderm's immunological defense mechanisms reported so far have used the sea urchin Strongylocentrotus purpuratus as a model, and information on the immunological defense mechanisms of Paracentrotus lividus and other sea urchins, is scarce. To remedy this gap in information, in this study, flow cytometry was used to evaluate several cellular immune mechanisms, such as phagocytosis, cell cooperation, and ROS production in P. lividus coelomocytes after PAMP stimulation. Two cell populations were described. Of the two, the amoeboid-phagocytes were responsible for the phagocytosis and ROS production. Cooperation between amoeboid-phagocytes and non-adherent cells resulted in an increased phagocytic response. Stimulation with several PAMPs modified the phagocytic activity and the production of ROS. The premise that the coelomocytes were activated by the bacterial components was confirmed by the expression levels of two cell mediated immune genes: LPS-Induced TNF-alpha Factor (LITAF) and macrophage migration inhibitory factor (MIF). These results have helped us understand the cellular immune mechanisms in P. lividus and their modulation after PAMP stimulation.     

Jewel of the seabed: sea cucumbers as nutritional and drug candidates

Marine sources have been attracting the attention of scientists and manufacturers worldwide hoping to find new alternatives for biological active substances. Promising new research indicates that sea cucumber, which is slug-like in appearance and has been a staple in Japan, China and other parts of East Asia since ancient times, is beginning to gain popularity as a dietary supplement in western countries. The roles of sea cucumber extracts in various physiological functions have spurred researchers to investigate the ability of sea cucumber to be an alternative in neutraceutical and medical applications. This article provides a brief introduction to sea cucumber and reviews its numerous bioactive compounds, such as triterpene glycosides, glycosaminoglycans, gangliosides, collagen, branched-chain fatty acid and lectins, which serve as potential sources of neutraceutical, pharmaceutical and cosmetic agents, thus providing a new platform in biochemical research.     

海参胶囊盐分测定检验方法的改进

根据GB/T 12457中氯化钠的测定,针对海参提取物的样品特性,进行煮制法、沉淀蛋白法和灰化法三种前处理改进,再直接滴定法测定样品中的盐分含量。通过实验对比,得出灰化法操作简便、测量结果准确度高等优点,适用于海参胶囊中盐分的测定,对样品能起到控制盐分的目的。