多个SPF鸡品系的RAPD分析

目的用RAPD方法分析三个品系SPF鸡的遗传变异,以指导SPF鸡群的育种工作.方法对三个SPF鸡品系F、M及B系和对照H纯系分别采血样30个,等量混匀为三个混合样,提取DNA,筛选随机引物,优化反应体系后进行PCR扩增,计算品系内平均相似系数,根据品系间遗传距离进行聚类分析并绘制树状聚类图.结果60个随机引物中有13个产生清晰稳定的多态条带,B系品系内平均相似系数为0.903,F系和M系分别为0.673和0.755,H系为0.968;聚类分析的结果是每个品系的三个样品先聚为一类,然后F系与M系、B系与对照H系分别聚为一类.结论F系和M系的纯合程度均较低,而B系较高;F系与M系的遗传距离较近,而与B系之间距离较远,三者亲缘关系以F系与M系较近,这与各个品系的引种背景相一致.     

Center for the Polyurethanes Industry summary of unpublished industrial hygiene studies related to the evaluation of emissions of spray polyurethane foam insulation

Spray polyurethane foam (SPF) insulation is used as thermal insulation for residential and commercial buildings. It has many advantages over other forms insulation; however, concerns have been raised related to chemical emissions during and after application. The American Chemistry Council's (ACC's) Center for the Polyurethanes Industry (CPI) has gathered previously unpublished industrial hygiene air sampling studies submitted by member companies that were completed during an eight-year period from 2007–2014. These studies address emissions from medium density closed cell and low density open cell formulations. This article summarizes the results of personal and area air samples collected during application and post application of SPF to interior building surfaces in both laboratory and field environments. Chemicals of interest included: Volatile Organic Compounds (VOCs), methylene diphenyl diisocyanate (MDI), flame retardants, amine catalysts, blowing agents, and aldehydes. Overall, the results indicate that SPF applicators and workers in close proximity to the application are potentially exposed to MDI in excess of recommended and governmental occupational exposure limits and should use personal protective equipment (PPE) consisting of air supplied respirators and full-body protective clothing to reduce exposure. Catalyst emissions can be reduced by using reactive catalysts in SPF formulations, and mechanical ventilation is important in controlling emissions during and after application.     

Sulfated modification can enhance the adjuvanticity of lentinan and improve the immune effect of ND vaccine

The purpose of this research is to study the effect of sulfated lentinan (sLNT) on immune effect of Newcastle disease (ND) vaccine. In immune response test, 14-day-old chickens were vaccinated with ND vaccine then grouped respectively to inject three sLNTs at two doses, once a day for three successive days, taking non-sulfated lentinan (LNT) as the control. The changes of serum antibody titer and peripheral lymphocyte proliferation were determined before and after vaccination. In immune protection test, 35-day-old chickens were challenged with ND virus (NDV) after treated similar to above mentioned. The morbidity and mortality of chickens were observed, and the changes of serum antibody titer and peripheral lymphocyte proliferation were measured before and after challenge. The results showed that three modified sLNTs could significantly enhance serum antibody titer and promote lymphocyte proliferation in two experimental chickens, and reduce morbidity and mortality of chickens challenged with NDV, which were better than that of non-modified LNT. Their high doses in enhancing antibody titer and low doses in promoting lymphocyte proliferation were more preferable. These results indicated that sulfated modification could enhance the adjuvanticity of LNT and improve the immune effect of ND vaccine. sLNT₂ possessed the best efficacy and would be expected as the candidate of a new-type immune adjuvant.     

Dietary cholesterol lowers plasma and cecal equol concentrations in mice

This study examined the effects of cholesterol on mouse intestinal microflora and on isoflavonoids in the cecum and plasma. Dietary cholesterol affects bile acid metabolism and bile acids can influence the intestinal microorganisms. Intestinal microflora appear to play an important role in isoflavone metabolism. We hypothesized that dietary cholesterol changes the metabolism of isoflavonoids and intestinal microorganisms in mice. Male mice were randomly divided into two groups, which were fed a cholesterol-daidzein (CDA) or daidzein (DA) diet (control diet) for 60d. Plasma equol and cecal equol concentrations were significantly higher in the DA group (control group) than in the CDA group. However, plasma cholesterol concentrations were significantly higher in the CDA group compared to the DA group. The composition of cecal microorganisms differed between the two dietary groups. The occupation ratios of Clostridium cluster XI, Clostridium subcluster XIVa, and Lactobacillales were significantly higher in the CDA group. The occupation ratio of Bifidobacterium was significantly lower in the CDA group. This study suggests that dietary cholesterol has the potential to affect the metabolism of equol from daidzein by altering the metabolic activity of the intestinal microorganisms and gut physiological function.     

Sun protection factor persistence on human skin during a day without physical activity or ultraviolet exposure

Background/purpose: Recently, we showed that the sun protection factor (SPF) decreases by a constant factor to reach 55% during a day with activities. Organic sunscreens but not inorganic ones are absorbed through the skin. We wished to determine the SPF decrease caused by absorption by investigating the difference in SPF decreases between an organic and an inorganic sunscreen, assuming that the sunscreens are stable, and that the SPF decrease is time dependent if caused by absorption.
Methods: Sunscreens were used on the backs of 22 participants, who were physically inactive at 22 °C. SPF testing was performed 30 min, 4, and 8 h after application of 2 mg/cm² sunscreen. Whether cream evaporation changed the ultraviolet (UV) transmission was studied in vitro .
Results: The SPFs of the organic and inorganic sunscreens were reduced by about 25% after 8 h. Evaporation of the cream did not cause a change in UV transmission in vitro .
Conclusion: A similar decrease in SPF of the organic and inorganic sunscreen was seen during 8 h without activities, and is thus not likely to be caused by absorption or evaporation from the skin. The SPF decrease after 8 h is about 55% when the participants perform activities and 25% without activities.
Trial registration: Registered at http://www.clinicaltrials.gov . Register name: 'Sunscreen: Persistence of Sun Protection Factor and the Influence on Vitamin D'. Register number H-B-2007-120.     

Antifibrotic effects of Artemisia capillaris and Artemisia iwayomogi in a carbon tetrachloride-induced chronic hepatic fibrosis animal model

Ethnopharmacological relevance

Artemisia capillaris and Artemisia iwayomogi, both members of the Compositae family, have been indiscriminately used for various liver disorders as traditional hepatotherapeutic medicines in Korea for many years.

Aim of the study

In this study, the anti-hepatofibrotic effects of Artemisia capillaris and Artemisia iwayomogi were comparatively analyzed using a carbon tetrachloride (CCl₄)-induced liver fibrosis rat model.

Materials and methods

Hepatic fibrosis was induced via a 10-week course of intraperitoneal CCl₄ injections (50% dissolved in olive oil, 2 mL/kg, twice per week). Water extract of Artemisia capillaris (AC) or Artemisia iwayomogi (AI) was orally administered six times per week from the 5th to the 10th week.

Results

AI (50 mg/kg) significantly attenuated the CCl₄-induced excessive release of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) in serum (p < 0.05), and hydroxyproline and malondialdehyde (MDA) contents in liver tissue (p < 0.05). Further, AI markedly ameliorated the depletion of total antioxidant capacity (TAC), glutathione (GSH), and superoxide dismutase (SOD) in liver tissue (p < 0.01). Unexpectedly, AC did not exert any effects on the above parameters. Histopathological and immunohistochemical analyses revealed that AI drastically reduced inflammation, necrosis, fatty infiltration, collagen accumulation, and activation of hepatic satellite cells in liver tissue. These changes were not observed with AC treatment. Several critical genes of fibrosis-related cytokines including transforming growth factor beta (TGF-β), platelet-derived growth factor beta (PDGF-β), and alpha smooth muscle actin (α-SMA) were more prominently downregulated by AI compared to AC treatment.

Conclusion

Our results show that AI exerts greater hepatoprotective and anti-fibrotic effects as compared with AC via enhancing antioxidant capacity and downregulating fibrogentic cytokines.     

肝癌组织中DNA倍体、SPF、PI值测定的临床意义

目的 了解原发性肝癌组织中DNA倍体,SPF、PI值,与临床分期的关系。方法应用流式细胞仪（FCM）测定石蜡包埋的原发性肝细胞癌组织标本120例,检测DNA倍体,SPF、PI值。结果随着原发性肝细胞癌临床分期的发展,临床Ⅰ～Ⅱ与Ⅲ～Ⅳ期肿瘤的异倍体率（Ⅰ～Ⅱ33．3％,Ⅲ-Ⅳ期42．8％）,SPF、PI值均增高,其与正常肝组织均有统计学意义。但肿瘤各临床分期Ⅰ-Ⅱ与Ⅲ-Ⅳ期之间并无统计学意义。结论测定原发性肝细胞癌组织的DNA倍体,SPF、PI值能了解其某些分子生物学特性,从而从分子水平上了解其增殖活性。对肿瘤的诊断及预后的评估有积极的临床意义。     

Rodent models to study the relationships between mammals and their bacterial inhabitants

Laboratory rodents have been instrumental in helping researchers to unravel the complex interactions that mammals have with their microbial commensals. Progress in defining these interactions has also been possible thanks to the development of culture-independent methods for describing the microbiota associated to body surfaces. Understanding the mechanisms that govern this relationship at the molecular, cellular, and ecological levels is central to both health and disease. The present review of rodent models commonly used to investigate microbial-host “conversations” is focused on those complex bacterial communities residing in the lower gut. Although many types of pathology have been studied using gnotobiotic animals, only the models relevant to commensal bacteria will be described.