Survivin在T细胞非霍奇金淋巴瘤组织中的表达及临床意义

【目的】了解生存蛋白Survivin在T细胞非霍奇金淋巴瘤（T—NHL）中的表达，以及Survivin表达与预后的关系。【方法】用免疫组化S-P法检测83例T细胞NHL组织Survivin表达情况，分析Survivin表达与预后的关系。【结果】Survivin表达阴性10例（12％），表达阳性73例（88％），Survivin表达与病理类型、性别、年龄、B症状、结外病变数、PS、分期、LDH水平、IPI等临床特征均无相关性（P〉0．05）。78例接受了化疗，大多数采用CHOP方案化疗，全组CR率45％，有效率86％，Survivin阴性者CR率60％，阳性者43％（P〉0．05）。中位随访22月（1—76月），全组5年生存率31％，Survivin表达阳性的T—NHL患者5年生存率明显低于Survivin表达阴性患者（21％比70％，P〈0．001）。多因素分析显示Survivin表达是T细胞NHL的独立预后指标。【结论】Survivin在T细胞NHL表达上调，Survivin阳性T—NHL患者5年生存率低于Survivin阴性者，Survivin表达可能为T细胞NHL预后的独立指标。     

外科治疗急性胆源性胰腺炎

目的探讨急性胆源性胰腺炎（ABP）手术时机和术式的选择.方法回顾性分析247例急性胆源性胰腺炎的临床资料.结果非手术治疗10例,死亡4例;12例急诊手术后发生并发症5例,死亡2例;169例延期手术术后发生并发症1例,治愈;56例择期手术无并发症发生.结论以胆道梗阻为主的ABP应急诊手术解除胆道梗阻;胆道无梗阻先采用非手术治疗,胰腺炎控制后,再处理胆道病变.     

Isolation and differentiation of embryonic stem cells from BALB/c mouse

Objective To invest the efficient method which can culture and induce embryonic stem cells to neurocyte in vitro. Methods Isolate the blastula of 3.5 d from BALB/c species mouse. Culture the cells from inner cell mass (inner cell mass, ICM) which were isolated by mechanical method on the mouse embryonic fibroblaste cell (MEF) feeder layer or 0.1% gelatin coated dishes. The stem cells were identified by characterized morphology, alkaline phosphatase stain, differential potency in vivo and immunochemistry stain. The isolated cells were differentiated by serial induction method that mimicking the intrinsic developmental process of the neural system. Results The isolated cells were positive for alkaline phosphatatse and SSEA-1 (stage specific embryonic antigen 1). Moreover they were identified pluripotent by differentiation in vivo. Therefore the isolated cells presented the characters of ESCs. Then the isolated cells were able to differentiate into neurocytes in vitro. Conclusion Mouse embryonic stem cells isolation, culture and differentiation system has been established.     

Neurosteroid modulation of allopregnanolone and GABA effect on the GABA-A receptor

The neurosteroid allopregnanolone (ALLO) or 3α-OH-5α-pregnane-20-one interacts with the GABA type A receptor chloride ion channel complex and enhances the effect of GABA. Animal and human studies suggest that ALLO plays an important role in several disorders including premenstrual syndrome, anxiety, and memory impairment. In contrast to ALLO, steroids with a hydroxy group in the 3β position usually exert a reducing effect and have recently attracted interest due to their suggested role in counteracting the negative action of ALLO. In this study, five different 3β-steroids were tested for their ability to modulate GABA-mediated chloride ion uptake in the absence and presence of ALLO in rat brain microsacs preparations. In addition, the effects of the 3β-steroids and their interaction with ALLO were investigated by patch-clamp recordings of spontaneous inhibitory postsynaptic currents (sIPSCs) in rat hypothalamic neurons from the medial preoptic nucleus (MPN). All tested 3β-steroids reduced the ALLO-enhanced GABA response in cerebral cortex, in hippocampus and in MPN. In cerebellum, only one had this effect. However, in the absence of ALLO, two of the 3β-steroids potentiated GABA-evoked chloride ion uptake and prolonged the sIPSCs decay time, whereas the others had little or no effect. Therefore, it is possible that at least some 3β-steroids can act as positive GABA_A receptor modulators as well as negative modulators depending on whether or not ALLO is present. Finally, these results suggest that the 3β-steroids could be of interest as pharmacological agents that could counteract the negative effects of ALLO.     

DNA damage by the glycation products of glyceraldehyde 3-phosphate and lysine

Summary In order to evaluate whether base modifications, apurinic/apyrimidinic site formation, strand breaks, or a combination of these lesions results from the interaction of glycation products with DNA, plasmid DNA was first reacted with these products, and then subjected to digestion with endonuclease III and endonuclease IV of Escherichia coli. Analysis of the differential effects of digestions with these enzymes by electrophoresis on agarose gels demonstrated that reactive glycation products produce both base modification and apurinic/apyrimidinic sites in DNA, in addition to the strand breaks observed after incubation with glycation products alone. These types of DNA damage may occur in specific diabetic cells where elevated levels of glycating sugars are associated with pathologic dysfunction. [Diabetologia (1994) 37: 145–149] Received: 28 May 1993 and in revised form: 30 August 1993     

补肾化瘀法延缓衰老机理研究─—六味丹坤方对老年小鼠自由基代谢的影响

选用自由基代谢为指标，对比观察了补肾六味地黄汤、化瘀丹坤方及补肾化瘀六味丹坤方的延缓衰老作用。实验结果表明：（１）六味地黄汤及丹坤方均能明显增加老年小鼠红细胞ＣＡＴ的活性，增加ＮＰＳＨ的含量，减少肝脑ＭＤＡ含量，即明显改善老年小鼠的自由基代谢紊乱；丹坤方尚能明显增强老年小鼠ＳＯＤ及ＧＳＨ－Ｐｘ的活性；（２）补肾化瘀六味丹坤方的作用明显优于单纯补肾的六味地黄汤或单纯化瘀的丹坤方；（３）补肾化瘀是更为有效的延缓衰老手段，并为衰老的“正虚挟瘀”观点提供了实验证据。     

角膜接触镜对泪膜流动和破裂时间的影响

观察５０例长期配戴亲水性角膜接触镜者戴镜前后ＳｃｈｉｒｍｅｒＩ试验和泪膜破裂时间（ＢＵＴ）的变化。结果表明，戴接触镜后ＢＵＴ缩短，ＳｃｈｉｒｍｅｒＩ下降，双眼ＢＵＴ与ＳｃｈｉｒｍｅｒＩ之间无相关性。讨论了引起泪膜流动和ＢＵＴ改变的机制。     

显微外科技术在尿道下裂修复中的应用

采用显微外科技术行阴囊纵膈皮瓣尿道下裂修复术18例，均获成功。其手术要点为：将阴茎腹侧纤维索条切除，充分伸展阴茎，皮瓣保留足够长度与宽度，克保血运；在显微镜下采用7－0尼龙丝线缝合，形成皮管，不缝皮肤，只缝皮下。硅胶管支架引流，加压包扎阴茎。本文对手术成功的原因进行了初步讨论。     

补中益气汤对“脾虚”大鼠胃粘膜易损伤性的复健作用研究

给大鼠灌胃大黄水提取液１０天，造成类“脾虚”模型进行胃粘膜损伤试验。结果表明该模型对消炎痛、应激因素引起的胃粘膜损伤指数明显高于正常对照组，经补中益气汤治疗７天，其胃粘膜损伤指数较非治疗组明显下降，提示“脾虚”大鼠胃粘膜易损伤性增高，补中益气汤对其有复健作用。     

Can supplementation of diet with omega-3 polyunsaturated fatty acids reduce coronary angioplasty restenosis rate?

The objective of this blinded, randomized, prospective study was to assess whether supplementation of normal diet with omega-3 polyunsaturated fatty acids can reduce angiographically defined restenosis following coronary angioplasty. The study included all patients undergoing coronary angioplasty in this institution between January 1988 and January 1989. One hundred and twenty patients enrolled, 60 in each treatment group. All were randomized to either supplementation of normal diet with 3 g of omega-3 polyunsaturated fatty acids per day started 1-2 days prior to angioplasty and continued for 6 months (treatment group), or to receive standard therapy only (control group). Quantitative angiographically defined restenosis was assessed at 6 months post angioplasty. Restenosis occurred in 27.8% (95% CI 18.0-37.7%) of lesions in the treatment group and in 28.3% (CI 16.9-39.7%) of lesions in the control group, but the difference was not statistically significant. The study showed that diet supplemented with 3 g of omega-3 polyunsaturated fatty acids started 1-2 days preceding angioplasty does not reduce angiographically defined restenosis rate.