黄芪注射液对严重创伤患者的免疫调节

目的　探讨黄芪注射液对严重创伤患者的免疫调节作用。方法　选择 5 0例严重创伤患者 (ISS ,创伤严重度评分≥16 ) ,检测应用黄芪注射液 ( 4 0ml)前后T淋巴细胞亚群血清IL 2及可溶性白介素 2受体 (SIL 2R)。结果　严重创伤患者伤后IL 2、CD4 +和CD4 +/CD8+减低 ,而SIL 2R和CD8+升高 ,黄芪治疗组在伤后 14d血中IL 2、T淋巴细胞CD4 +亚群、CD4 +/CD8+降低及SIL 2R、CD8+升高程度均低于治疗前 ,P <0 .0 5。结论　严重创伤患者伤后细胞免疫功能明显抑制 ,应用黄芪注射液可以增加机体免疫功能     

原发性肾小球疾病患者T淋巴细胞亚群变化的初探

本文应用磁性磷酸酶－抗碱性磷酸酶桥联酶标技术（APAAP），对27例原发性肾小球疾病患者外周血T淋巴细胞亚群进行观察。结果表明：本组病例表现为CD细胞升高，CD细胞减少，和CD／CD比值增加。提示原发性肾小球疾病可导致细胞免疫功能改变，T淋巴细胞亚群的变化，是肾组织损害的一个间接证据，可作为临床诊断一个有价值的参考指标。     

高浓度吗啡对体外培养的小鼠淋巴细胞增殖的抑制效应

高浓度吗啡对体外培养的小鼠淋巴细胞增殖的抑制效应北京医科大学药理学教研室陆正武，林志彬利用不同刺激原诱导的淋巴细胞增殖反应，研究吗啡的体外作用机制。结果表明：（１）高浓度吗啡（≥０．１ｍｍｏｌ·Ｌ－１）以剂量依赖方式，抑制ＣｏｎＡ／ＬＰＳ所致淋巴细胞...     

CD4+ lymphocyte subsets in the cerebrospinal fluid of multiple sclerosis and non-inflammatory neurological diseases

Summary We studied paired cerebrospinal fluid (CSF) and peripheral blood (PB) samples from 18 inactive multiple sclerosis (MS) patients and 10 with non-inflammatory neurological diseases. By means of a dual-colour cytofluorimetric micromethod we were able to count 1500 cells on average in each CSF sample. We found a significant reduction of CD45RA+ and CD4+CD45RA+ cells in the CSF of MS patients. Similarly, CD45RA+ and CD4+CD45RA+ CSF/PB ratios were lower compared with controls. The reduction of suppressor-inducer T-cells did not correlate with CD8+ cell levels in the CSF. The CD4+ subset ratio (CD4+CD45RA–/CD4+CD45RA+) was significantly increased in the CSF of MS patients. Our data suggest that the reduction of CD4+CD45RA+ cells in the PB is not due to a segregation of such cells in the CSF. Conversely, CSF changes reflect changes in the PB similar to these found for other T-cell subsets.     

脑瘤患者胸腺移植前后几项免疫指标的观察

本文报道脑瘤病人胸腺移植前后细胞免疫和体液免疫的几项常规指标。所有接受移植的患者均未出现任何免疫排斥反应或移植物抗宿主反应。将病人随机分为两组,一组经穿刺移植;一组以手术植入。两组患者移植前、后测定淋巴细胞数、淋巴细胞转化率及 E-玫瑰花环形成率均呈现极显著性差异(P<0.001)。血清中 IgG 和 C_3也呈现显著差异(P<0.05)。     

Route of lymphocyte migration through the high endothelial venule (HEV) in human palatine tonsil

Eleven palatine tonsils were collected from subjects who underwent tonsillectomy in Christian Medical College Hospital and the route of migration of lymphocytes through the high endothelial vessel was studied under EM. In the interendothelial route, migration of a lymphocyte through HEV wall began with the adhesion of a lymphocyte to the surface of endothelial cells by means of a short cytoplasmic projection in the vicinity of intercellular space. The projection extended into the cleft between adjacent endothelial cells. The lymphocyte migrated through HEV by diapedesis. After the lymphocyte had traversed the interendothelial space, it occupied the subendothelial space. In the transendothelial route, migration of a lymphocyte through HEV was initiated by adherence of the lymphocyte to the endothelial cell. The adherent lymphocyte compressed or invaginated into the cytoplasm of the endothelial cell, entered the endothelial cell, was completely enclosed within the endothelial cell cytoplasm, and emerged from the endothelial cell to occupy the subendothelial space. Evidence is presented from static transmission electron microscopic pictures for the migration of lymphocytes by both interendothelial and transendothelial routes through the high endothelial venule.     

Effect of prostaglandin E1 on graft function of kidneys from living related donors

Prostaglandin E₁ (PGE₁) was used in renal transplant recipients with living related donors. The drug was given intravenously from day 1 to day 7 after transplantation at a dose of 40 µg/kg twice a day. A total of 45 patients were studied divided into two groups: 25 patients were treated with PGE₁ (group B) and the remaining 20 patients did not receive the drug (group A). In group B, 24-h creatinine clearance (Ccr) was 66 ± 12.8 ml/min compared with 40.3 ± 13.4 ml/min in group A on the fifth postoperative day (P < 0.05). Urinary levels of N-acetyl-β-d -glucosaminidase (NAG) and serum levels of platelet factor 4 (PF4) in group B were significantly lower than in group A. On the fourth postoperative day, the urinary excretion of thromboxan B₂ (TxB₂) in group A was higher than in group B, but not significantly (5.1 ± 3.0 ng/day and 2.8 ± 1.1 ng/day, respectively). Acute rejection occurred in four patients in group B and in 10 patients (40%) in group A. The percentage of Leu2a-positive lymphocytes in group B was higher than in group A. We conclude that postoperative administration of PGE₁ improves graft function in kidneys from living related donors.     

麻风病患者外周血相关免疫功能测定的临床意义

目的探讨麻风病患者外周血相关免疫分子的变化和临床意义。方法应用流式细胞分析仪，以荧光标记的鼠抗人单克隆抗体对16例麻风病患者外周血淋巴细胞CD3／CD4／CD8、CD3／CD(16 56)(NK)和CD3／HLA-DR进行测定。结果16例患者发病初期外周血淋巴细胞亚群中CD3^ ／CD4^ 、CD3^ ／HLA-DR^-检测值和CD4／CD8比值均分别低于健康组相应参数的下限，CD3^ ／CD(16 56)^ 和CD3^ ／HLA-DR^ 检测值分别高于健康组相应参数的上限、NK的检测值在正常范围内。13例患者治疗病情稳定后2-4周，CD3^ ／CD4^ 、CD3^ ／HLA-DR^-的检测值和CD4／CD8比值均逐步增高，CD3^ ／CD(16 56)^ 、CD3^ ／CD8^ 和CD3^ ／HLA-DR^ 稍有增高或变化不明显，NK的检测值稍有增高；3例患者病情波动时CD3^ ／CD4^ 、CD3^ ／HLA-DR^-检测值和CD4／CD8比值较稳定期明显下降，而活化T淋巴细胞CD3^ ／CD(16 56)^ 、CD3^ ／HLA-DR^ 和NK细胞的检测值较稳定期明显增高2倍以上。结论通过外周血相关免疫分子的动态观察，有助于检测病情变化，时及时治疗和评价疗效有重要意义。     

登革病毒感染后小鼠细胞免疫功能的动态变化

从登革Ⅱ型病毒感染成年ＢＡＬＢ／ｃ小鼠为模型，观察感染后不同时间机体细胞免疫反应的变化。结果表明：感染后７－１４ｄ免疫功能处于激活状态，表现为腹腔巨噬细胞的吞噬功能和脾淋巴细胞对ＣｏｎＡ的反应及ＩＬ－２产生水平都明显高于对照组，感染２１ｄ后免疫功能则转为抑制状态，上棕免疫反应明显低于对照组，脾中的Ｌ３Ｔ４＾＋细胞亚群的百分比逐渐下降；相反，ＬＹＴ２＾＋细胞亚群的百分比逐渐升高，这种抑制现象可维持到