姜黄素的提取、分离与测定

本文探讨了微波辅助提取姜黄素的最佳工艺条件,将其3种成份分离的分离条件及用HPLC法进行其含量的测定.结果 表明其最佳工艺条件为:辐射功率400 w,辐射时间为3 min,溶剂为75%乙醇溶液,剂料比为1:20.利用薄层色谱法筛选的最佳淋洗剂的组成为氯仿-甲醇-冰醋酸(125:5:2).HPLC法测定姜黄素在5～100 mg·L-1的范围内呈线性关系(r=0.99935),样品中姜黄素含量平均为3.2135%,约占总姜黄素的50%.     

十二烷基硫酸钠提取姜黄素的工艺优化

目的筛选出利用十二烷基硫酸钠(SDS)提取中药姜黄中姜黄素的最佳工艺条件。方法采用正交实验对提取溶剂乙醇的浓度、提取时间、SDS的浓度等条件进行优化。结果与传统乙醇回流提取方法比较,乙醇浓度由80%降低至65%,提取时间由2 h缩短至0.75 h。结论提取姜黄中姜黄素时,加入0.3%SDS,乙醇浓度为65%,两次回流提取时间为0.75h为最佳工艺条件。     

甘草对姜黄素提取率的影响

目的 研究甘草对姜黄中姜黄素提取率的影响.方法 甘草与姜黄按不同比例混合,采用不同浓度的乙醇提取,HPLC法测定提取液中姜黄素的含量,并计算姜黄素的提取率.结果 30%乙醇为提取溶剂时,甘草与姜黄(1:1)混合,姜黄素提取率可增加一倍.结论 甘草中的甘草酸是提高姜黄素提取率的主要活性物质.     

姜黄素对糖尿病及其微血管病变治疗研究的进展

糖尿病是危害人群健康的一种慢性疾病。糖尿病微血管并发症治疗的基础与临床研究始终是医学研究的一大热点。近年来姜黄素对糖尿病和糖尿病微血管并发症治疗作用的基础和临床研究已引起研究人员的重视。姜黄素可以显著的降低糖尿病动物模型血糖水平,参与调脂及抗氧化过程。姜黄素可以减弱多种诱发糖尿病微血管并发症的诱发因素,并且对靶器官有保护作用。     

姜黄素对大鼠早期肝缺血再灌注损伤肺的保护作用

目的 探讨姜黄素对大鼠肝脏缺血再灌注早期损伤(再灌注1、3 h)肺的保护作用.方法 将大鼠随机分为假手术组(A组)、对照组(B组)和实验组(C组).通过检测再灌注早期肺组织病理学的改变及肺组织中SOD、CAT、MDA、MPO的含量来评价姜黄素对大鼠肝脏缺血再灌注损伤肺的保护作用.结果 姜黄素可减少大鼠肝缺血再灌注损伤肺间隔的水肿和肺泡中红细胞和白细胞的渗出.姜黄素提高了早期缺血再灌注后肺组织中SOD、CAT含量,降低了肺组织中MDA、MPO含量.结论 姜黄素通过抑制肺组织中的氧自由基的生成及中性粒细胞的浸润,从而在早期大鼠肝缺血再灌注损伤中起到了对肺的保护.     

Preventive and curative effects of curcumin on the development of gastric inflammatory diseases in rats

Preventive and curative effects of curcumin on experimental acute and chronic gastric ulcers were investigated to validate its clinical application on a remedy for peptic ulcer. Intraduodenal administration of curcumin, 5–20 mg/kg, inhibited gastric acid secretion in pylorus-ligated rats, and oral administration prevented ethanol-induced acute gastric mucosal lesions. Curcumin (20–80 mg/kg, p.o.) dose-dependently prevented both serotonin-induced gastric mucosal lesions and compound 48/80-induced gastric mucosal lesions in rats. Furthermore, oral administration of curcumin, 10–80 mg/kg, twice daily for 10 days, significantly accelerated the healing of acetic acid-induced chronic gastric ulcer and promoted mucosal regeneration in the ulcerated portion in a dose-related manner. Cimetidine prevented the formation of ethanol-induced gastric mucosal lesions, but not of serotonin-induced and compound 48/80-induced gastric mucosal lesions. Consecutive administration of cimetidine showed a marked acceleration in the healing of acetic acid-induced ulcer. Aminoguanidine, an inducible nitric oxide synthase (iNOS) inhibitor, showed anti-ulcerogenic effects similar to those oberved for curcumin. The present results indicate that curcumin exhibits gastric cytoprotection in the acute lesion models and ulcer healing promotion in the chronic ulcer model. The preventive and curative effects of curcumin might be due to an increase in the mucosal defensive mechanism through its antioxidant property and inhibition of NO or cytokine-mediated inflammation.     

Effects of curcumin on reflux esophagitis in rats

The preventive effect of curcumin, a compound isolated from the rhizome of Curcuma longa, on experimental reflux esophagitis in rats was investigated in order to validate its potential therapeutic use for gastroesophageal reflux disease. Curcumin (20 mg/kg, i.d.), the antioxidative agent dimethyl sulfoxide (DMSO) (1 ml/kg, i.p.) or the proton pump inhibitor lansoprazole (1 mg/kg, i.d.) inhibited the formation of acute acid reflux esophagitis by 52.5, 61.5 and 70.9% respectively. Curcumin alone was not effective in preventing chronic acid reflux esophagitis, but the combination of curcumin and DMSO reduced the mortality rate and the severity of the esophagitis ulcer index to the same extent (56.5%) as did the lansoprazole (53.9%). Intraduodenal administration of curcumin also markedly prevented the formation of acute mixed reflux esophagitis, together with reducing the incidence or the severity of neutrophil infiltration, when compared to a control group. In contrast, lansoprazole tended to increase the severity of all histopathological changes, when compared to either the control or the curcumin-treated group. Aminoguanidine, a specific inducible nitric oxide synthase inhibitor, had no preventive effect against both types of acute reflux esophagitis models, and increased the mortality in the chronic acid reflux esophagitis model. From these results, it is indicated that curcumin can effectively prevent acute reflux esophagitis formation. Although curcumin is less potent than lansoprazole in inhibiting acid reflux esophagitis, it is superior to lansoprazole in inhibiting mixed reflux esophagitis. The antiulcerogenic mechanisms are considered to be closely associated with its antioxidant nature and antiinflammatory property.     

姜黄素防治慢性心力衰竭大鼠左心室胶原网络重构的实验研究

目的 观察姜黄素对慢性心力衰竭（CHF）大鼠左室胶原网络重构的影响。方法 采用缩窄大鼠腹主动脉的方法，将大鼠随机分成假手术组、手术对照组、卡托普利组和姜黄素组，姜黄素组又分为高、中、低3个剂量组，造模后每周测定鼠尾动脉血压，分别于造模后第12、20周观察左室质量指数、心脏血流动力学变化，采用VG染色方法检测心肌胶原含量。结果 12周末手术对照组LVEDP、LVMI、CVF-V、CVF—NV均明显强于其余各组，20周末与手术对照组相比，姜黄素各剂量组和卡托普利组大鼠LVSP均增加，LVEDP、LVMI以及CVF-V、CVF-NV的表达显著降低，姜黄素高剂量组尤为明显；不同时间段，假手术组LVEDP、LVMI、CVF-V、CVF-NV的前后表达相近，而手术对照组、卡托普利组和姜黄素各剂量组前后差别显著。结论 姜黄素具有抑制胶原网络重构的作用，可在一定程度改善心功能，延缓CHF的进程。     

姜黄素衍生物药理活性的研究进展

姜黄（Curcuma longa L.）为姜科姜黄属植物,药用其根茎,主产于中国、印度、日本等国家.1870年从姜黄属植物中分离出姜黄素,1910年Lampe等首先阐明了其化学结构.现已证明其具有抗肿瘤、抑炎、利胆、抗氧化、抗肝细胞毒性、抗风湿、抑菌、抗低血压、低胆固醇等广泛的药理作用.目前对其化学结构进行修饰已成为国内外研究的热点.本文就目前对姜黄素衍生物的研究进展做一综述.     

姜黄素预防大鼠肝纤维化效果的观察

目的：明确姜黄素对实验性肝纤维化的预防效果。方法：以四氯化碳制作大鼠肝纤维化模型，同时按每100g体重分别给予20mg、10mg、5mg姜黄素灌胃处理，设立正常组、肝纤维化组和阳性对照组；8周后处死大鼠，取血检测血清ALT、AST、HA、PC-Ⅲ、TGF-β、TNF-α水平；肝脏组织HE及Masson染色观察病理改变，根据SSS计分系统评估肝纤维化程度。结果：姜黄素可以减少肝纤维化大鼠血清中明显升高的ALT、AST、HA、PC-Ⅲ、TGF-β、TNF-α水平，明显减轻肝纤维化程度，SSS计分在姜黄素组明显降低，接近正常（P〈0．05），以上作用均随着姜黄素用量增大而加强。结论：善黄素可以预防四氯化碳所致的大鼠肝纤维化，改善肝功能．具有一守的量效关系。