旋覆花中鞣质的含量测定

目的测定旋覆花水提物中鞣质的含量。方法采用干酪素法和络合滴定法进行鞣质含量测定。结果干酪素法测定旋覆花中鞣质含量为5.96%,RSD=1.68%(n=3);络合滴定法测得含量为8.32%,RSD=2.09%(n=3)。结论该两种分析方法简便、准确,可用于旋覆花中鞣质的含量测定。     

盐酸尼非卡兰的有关物质及含量测定方法

 目的分别用HPLC和非水酸碱滴定法建立起盐酸尼非卡兰有关物质检查及含量测定的方法,为原料的质量控制提供有效的分析方法。方法采用岛津CLC-ODS(6mm×150mm,5μm)柱,流动相:乙腈-0.02mol·L^-1的磷酸二氢钾溶液(NaOH溶液调pH至5.0)=25∶75;检测波长:268nm;流速:1.0mL·min^-1;柱温:30℃。结果在选定的色谱条件下,盐酸尼非卡兰与7个有关物质分离完全;盐酸尼非卡兰在5～100mg·L^-1内,峰面积与浓度线性关系良好,r=0.9999;检测限为0.2ng。结论方法简便,准确,专属性强,可用于盐酸尼非卡兰的有关物质检查及含量测定。     

pH滴定法测定羟乙膦酸钠的含量

本文采用ｐＨ滴定法,以ｐＨ５．０为起始ｐＨ,ｐＨ８．２为终点指示ｐＨ,用ＮａＯＨ直接滴定,测定了羟乙膦酸钠的含量。该方法简便、准确快速,平均回收率为１００．７２％,ＲＳＤ为０．２２％（ｎ＝６）。     

盐酸环丙沙星水分的双溶剂费休氏法测定

采用以甲醇为基本溶剂,水为辅助溶剂的双溶剂费休氏法,可准确测定盐酸环丙沙星中分子内和游离状态的全部水分。其平均回收率为１００．０５％,ＲＳＤ为０．２５％,为溶解特性类似的药品提供了可靠的水分测定。     

Complex Formation Between The Anionic Polymer (PAA) and a Cationic Drug (Procaine HC1): Characterization by Microcalorimetric Studies

Purpose. Due to the importance of drug-polymer interactions in, inter alia, drug loading/release, supramolecular assemblies and DNA delivery for gene therapy, the aim of this study was therefore to establish the mechanism of interaction between a model polymer (Polyacrylic acid, PAA) and a model drug (procaine HCl). Methods. This was performed by studying the effect of salt (KCl) concentration on their heat released values using Isothermal Titration Microcalorimetry (ITM). The integrated released heat data were computer fitted to a one class binding model and the thermodynamic parameters (K_obs, H, and N) were determined. Results. As the KC1 concentration was increased, K_obs decreased thus establishing the salt dependence of the interaction. The linear variation of G_obs with S_obs indicated that their interaction was entropically driven. The stoichiometry of the interaction was calculated to be one procaine molecule per monomer of PAA. Dissection of the total observed free energy at each KC1 concentration indicated that the contribution of the non-electrostatic attractions to the interaction of PAA with procaine HC1 was greater than those of the electrostatic attractions. Conclusions. We have shown that the interaction between PAA and procaine HC1 is dependent upon the presence of counterions (monovalent ions) and is mainly entropically driven. The calculated stoichiometry indicated that one procaine HC1 molecule neutralised one carboxylic acid group on PAA. Although electrostatic interactions were necessary for initiating complex formation, the non-electrostatic forces were dominant in stabilising the PAA-procaine HC1 complex.     

硫酸软骨素的制备及含量测定

本文介绍了由猪软骨制备硫酸软骨素（ＣｈｏｎｄｒｏｉｔｉｎＳｕｌｆａｔｅ,ＣｈＳ）的方法。该方法工艺简便,成本较低,产品ＣｈＳ含量在７０％左右,收率为１４％（以骨粉计）。探讨了配位滴定法的准确度,并用该法测定了ＣｈＳ的含量。     

电位滴定法测定胃液氨的含量

根据甲醛能与NH_4~+反应生成等摩尔H~+的原理,用电位滴定法测定了胃液氨的含量。结果表明,该法的灵敏度高于传统的扩散法,回收率为92.5％—93.8％,可作为临床和科研工作中定量检测胃液氨浓度的可靠方法。     

滴定剂体积对酸碱双点电位滴定法准确度的影响

目的：探讨两次加入滴定剂标准溶液的体积对酸碱双点电位滴定法准确度的影响。方法：根据误差传递理论，用数学方法推导出影响测定结果准确度的因素。结果：滴定剂体积Ｖ１ 固定时，Ｖ２ 越大准确度越高；滴定剂体积Ｖ２ 固定时，Ｖ１ 有一最佳范围。结论：酸碱双点电位滴定法具有简便、快速等特点，通过选择适宜的滴定剂体积能够提高测定结果的准确度。     

国产GY—93型二氧化碳库仑滴定仪测定血清TCO2的方法学评价

探讨国产ＧＹ－９３型二氧化碳库仑滴定仪测定血清ＴＣＯ２的临床实用性。方法：使用该仪器测定一系列血清样本并与离子选择性电极法和手工酸碱滴定法的测定结果进行统计学比较。结果：库仑法在５～６０ｍｍｏｌ／Ｌ范围内有良好的线性，批内重现性高于酸碱滴定法（Ｐ＜０．０１）与电极法相当（Ｐ＞０．０５），批间重复性较差（ＣＶ＝９．８％），库仑法与电极法相关性较好ｒ＝０．９２。结论：该仪器批内精密度优于酸碱滴定法，但稳定性不够好，使得仪器的长期使用受限，尚需进一步完善。     

电位滴定-水溶液银量法测定盐酸林可霉素注射液中氯化钠的含量

目的　建立测定盐酸林可霉素注射液中氯化钠含量的方法。方法　电位滴定 -水溶液银量法结合HPLC外标法。在水溶液中 ,以Mettler复合银电极指示 ,用 0 .1mol·L- 1 硝酸银液滴定至电位发生突跃 ;测得结果扣除注射液中林可霉素盐酸盐对硝酸银滴定液的本底消耗 ,即得注射液中氯化钠的纯含量。结果　林可霉素A、B组分总含量在 6 .0～ 1 2 0 .2mg·ml- 1 范围内 ,溶液中林可霉素总浓度与硝酸银滴定液的消耗体积有良好的线性关系 ,r=0 .9999;方法重复性好 (RSD =0 .1 % ,n =5) ;准确度高 ,回收率为 99.2 % (RSD =0 .5 % ,n =6) ,加样回收率为1 0 0 .2 % (RSD =0 .4 % ,n =6)。结论　方法准确、便捷、适用 ,可准确测定盐酸林可霉素注射液中氯化钠的含量