全文获取类型
收费全文 | 7656篇 |
免费 | 651篇 |
国内免费 | 490篇 |
专业分类
耳鼻咽喉 | 22篇 |
儿科学 | 14篇 |
妇产科学 | 43篇 |
基础医学 | 525篇 |
口腔科学 | 102篇 |
临床医学 | 238篇 |
内科学 | 518篇 |
皮肤病学 | 106篇 |
神经病学 | 167篇 |
特种医学 | 71篇 |
外国民族医学 | 3篇 |
外科学 | 186篇 |
综合类 | 1387篇 |
现状与发展 | 1篇 |
预防医学 | 577篇 |
眼科学 | 96篇 |
药学 | 2095篇 |
中国医学 | 2405篇 |
肿瘤学 | 241篇 |
出版年
2024年 | 19篇 |
2023年 | 76篇 |
2022年 | 159篇 |
2021年 | 234篇 |
2020年 | 222篇 |
2019年 | 178篇 |
2018年 | 199篇 |
2017年 | 303篇 |
2016年 | 319篇 |
2015年 | 321篇 |
2014年 | 559篇 |
2013年 | 679篇 |
2012年 | 610篇 |
2011年 | 688篇 |
2010年 | 532篇 |
2009年 | 433篇 |
2008年 | 469篇 |
2007年 | 454篇 |
2006年 | 349篇 |
2005年 | 281篇 |
2004年 | 218篇 |
2003年 | 207篇 |
2002年 | 187篇 |
2001年 | 129篇 |
2000年 | 84篇 |
1999年 | 101篇 |
1998年 | 82篇 |
1997年 | 76篇 |
1996年 | 68篇 |
1995年 | 59篇 |
1994年 | 64篇 |
1993年 | 52篇 |
1992年 | 58篇 |
1991年 | 34篇 |
1990年 | 31篇 |
1989年 | 40篇 |
1988年 | 27篇 |
1987年 | 18篇 |
1986年 | 15篇 |
1985年 | 22篇 |
1984年 | 23篇 |
1983年 | 22篇 |
1982年 | 18篇 |
1981年 | 16篇 |
1980年 | 15篇 |
1979年 | 15篇 |
1978年 | 7篇 |
1977年 | 6篇 |
1976年 | 5篇 |
1975年 | 4篇 |
排序方式: 共有8797条查询结果,搜索用时 31 毫秒
21.
R. Bendayan G. Kennedy R. C. Frecker E. M. Sellers 《European journal of clinical pharmacology》1993,44(1):51-55
Summary The efficacy of cimetidine as a treatment that could reduce smoking in heavily dependent smokers has been determined. In a randomised, double-blind, double-crossover experiment, 43 heavy smokers were divided into two groups, one receiving cimetidine 400 mg orally three times a day, and the other receiving placebo for two weeks followed by the alternative treatment (placebo or cimetidine).No significant difference in the mean alveolar carbon monoxide, nicotine or cotinine levels was found between the two treatment groups compared to baseline. Since the alveolar carbon monoxide level reflects the intensity of smoking behaviour, the results suggest that no change in smoking behaviour occurred in the subjects.Contrary to our previous findings that cimetidine decreased the total body clearance of nicotine by 30% in a population of non-smokers, in the heavily dependent smokers, cimetidine did not appear to alter nicotine elimination. One possible explanation for the discrepancy is that tobacco smoking is known to induce nicotine metabolism and the induction might have offset any effect of cimetidine on nicotine elimination.Cimetidine does not appear to be a useful treatment leading to a reduction or cessation of cigarette smoking. 相似文献
22.
The hypoglycaemic effect of Anthocleista vogelii was studied in mice, rats and rabbits. Aqueous extract of the plant obtained by infusion from finely pulverized root was used. The extract (100, 400 and 800 mg/kg) induced significant hypoglycaemic activity in a dose-related fashion at 2 h after oral administration in mice and rats with ED25 of 250 mg/kg and 350 mg/kg respectively. The extract (800 mg/kg, orally) similarly induced statistically significant lowering of blood glucose levels at 8 h in normoglycaemic rabbits. The extract (400 mg/kg and 800 mg/kg, orally) also caused reduction of blood glucose levels in alloxan-induced diabetic animals. The results of this study indicate that the aqueous extract of the roots of Anthocleista vogelii possess favourable hypoglycaemic activity both in normo and hyperglycaemic animals compared to chlorpropamide as a standard. 相似文献
23.
仙人掌提取物对小鼠血糖的影响 总被引:1,自引:0,他引:1
目的 :探讨仙人掌水性和酸性提取物对小鼠血糖的影响。方法 :糖尿病小鼠造模成功后分为 5组 :仙人掌酸提物 2个剂量组 (0 .5g·kg-1·d-1和 1.0g·kg-1·d-1) ,水提物 2个剂量组和优降糖组 (2 0 0mg·kg-1·d-1)。正常小鼠灌注等量生理盐水作为正常对照。 6组小鼠均连续给药 2 1d后测血糖。结果 :给药后仙人掌酸提物 2个剂量组血糖均较给药前降低 (P均 <0 .0 5 ) ,2个剂量组间差异无统计学意义 (P >0 .0 5 ) ,但 2组与优降糖组相比 ,P均 <0 .0 5 ;水提物 2个剂量组给药前后血糖无明显变化 (P >0 .0 5 )。结论 :仙人掌酸提物有降血糖作用 相似文献
24.
K. M. Bijli† B. P. Singh S. Sridhara S. N. Gaur‡ N. Arora 《Clinical and experimental allergy》2003,33(1):65-71
BACKGROUND: Allergen extracts are unstable, heat labile or susceptible to proteases. Stability of allergen extracts is important for proper diagnosis and therapy of allergic disorders. OBJECTIVE: The present study was undertaken to determine the preservation and stabilization conditions of Imperata cylindrica (Ic) grass pollen extract. METHODS: The Ic extract was kept with 0.1 mepsilon-aminocaproic acid (EACA), 0.75 m sucrose, 5% glycerol, 0.03% human serum albumin (HSA) or 0.4% phenol for different time periods. The extracts were stored for 3, 6 and 12 months each at 4 degrees C, 4 degrees C with daily exposure to room temperature (RT) for 1 h, and RT. The quality of extracts was analysed by SDS-PAGE, Western blot, ELISA, ELISA inhibition and skin test. RESULTS: Extracts kept with EACA and sucrose retained most of the protein bands followed by glycerol as determined by SDS-PAGE and Western blot during all storage periods and conditions in comparison with standard extracts. The extracts kept with HSA, phenol and without preservative (WP) showed protein degradation below 33 kDa after 3 months storage at all conditions. However, a 67-kDa allergen was stable in these extracts. EACA extract required 75 to 120 ng of protein for 50% inhibition in IgE binding under different conditions, whereas standard extract required 70 ng for the same. ELISA also demonstrated high allergenic reactivity of EACA extract. ID test on allergy patients with EACA extract demonstrated same allergenic potency as that of standard extract. CONCLUSION: EACA is the best preservative/stabilizing agent of Ic pollen extract, followed by sucrose and glycerol. Ic extract kept with phenol, HSA and without preservative showed degradation within 3 months. EACA preserved extract is equally potent as that of standard extract up to 1 year's storage. 相似文献
25.
100mg香烟烟油,多氯联苯诱导大鼠肝S_9组分中芳香烃羟化酶代谢B[a]P为3-OH-B[a]P的活性分别升高约40,20倍,而代谢B[a] P生成6-OXY-B [a]P自由基产物却仅由多氯联苯诱导的S_9组分作用中才被观察到。 相似文献
26.
目的 :探讨蝌蝌提取液 (T871)对白血病细胞的诱导分化作用。方法 :利用细胞生长曲线、形态学观察、细胞免疫化学及原位mRNA杂交和完整细胞原位斑点印迹技术 ,观察了T871诱导HL 60细胞分化的作用和分化过程中癌基因c myc、c myb表达的变化。结果 :T871能抑制HL 60细胞的增殖 ,形态学表现为类似单核 /巨噬细胞 ,同时T871还能使HL 60细胞的NBT还原能力、ANAE活性显著增强 ,同时伴有癌基因c myc、c mybmRNA表达的下调。结论 :T871能诱导HL 60细胞向单核 /巨噬细胞方向分化 ,c myc、c myb癌基因可能参与了此过程 相似文献
27.
Yuan Xue Tracy L. Williams Ting Li Joshua Umbehr Liang Fang Weiqun Wang Richard C. Baybutt 《Toxicology in vitro》2005,19(8):1061-1069
This study was to evaluate the effects of cigarette smoke constituents upon type II pneumocyte surfactant production in vitro, and how vitamins A and E may alter the response. Freshly isolated type II pneumocytes from Sprague–Dawley rats were incubated 20 h in medium with fetal bovine serum that was or was not treated with cigarette smoke. The number of adherent cells was inversely related to the dose of smoke or benzo(a)pyrene. Despite the decreased number of treated cells, the total amount of surfactant per culture well was unchanged, whereas surfactant production per cell was significantly increased (P < 0.05). Vitamin A concentration was significantly (P < 0.05) lower in the smoke-treated serum compared with untreated serum. When vitamin A or E was added to the cigarette smoke-treated serum, cell adherence and surfactant production returned to control values. In conclusion, cigarette smoke constituents or benzo(a)pyrene alone decreased the number of adherent type II pneumocytes, but did not alter surfactant amounts because of an increased production of surfactant per cell. Type II pneumocytes seem to adjust surfactant production dependent upon the number of type II pneumocytes to produce it and vitamin A or E enhance cell attachment in the presence of the smoke toxins. 相似文献
28.
用~3H-TdR掺入法观察不同稀释度的正常人与重症肌无力患者胸腺提取液对健康成人外周血淋巴细胞增殖的影响。实验证实了正常儿童的胸腺功能下降(高浓度P<0.02,余浓度P>0.05),成人胸腺无功能(P>0.05);并且得出增生型重症肌无力胸腺提取液对PHA诱导的人外周血淋巴细胞增殖有促进和抑制双向作用。提示重症肌无力患者的胸腺免疫活性与正常人比较有差异。解释并肯定了手术切除胸腺治疗增生型重症肌无力的作用。 相似文献
29.
荔枝壳红色素的提取及稳定性观察 总被引:4,自引:0,他引:4
以荔枝壳为原料,在酸性条件下浸提荔枝壳红色素,并对其稳定性进行了研究,结果表明,该色素在酸性条件下对光、热、常用食品添加剂均具有较好的稳定性。 相似文献
30.
利用响应面分析法对影响酵母抽提液中总氮截留率和海藻糖透过率的主要因素进行分析,优化超滤工艺,得到最佳超滤条件:料液质量浓度10 g/dL,料液温度42℃,操作压力0.31MPa,在最佳超滤条件下得到酵母抽提物蛋白质截留率96.5%,海藻糖透过率94.8%,所得RSA图可直观地反映各因素与蛋白氮截留率和海藻糖透过率的关系. 相似文献