氯霉素酒精湿敷用于肿瘤患儿PICC局部感染的护理

目的:探讨肿瘤患儿PICC局部感染的护理方法。方法:将2010年1月~2014年1月我科收治的发生PICC局部感染的70例肿瘤患儿随机分为对照组和试验组。对照组30例,采用抗生素软膏局部涂擦,试验组40例采用氯霉素酒精湿敷。比较两组局部感染治愈时间。结果:试验组局部感染治愈时间较对照组短,差异有统计学意义(P0.05)。结论:肿瘤患儿PICC局部感染采用氯霉素酒精湿敷,可缩短感染治愈时间。     

紫外-可见分光光度法测定氯霉素炉甘石洗剂中氧化锌的含量

目的：建立紫外-可见分光光度法测定氯霉素炉甘石洗剂中氧化锌含量的方法。方法：采用锌试剂显色,紫外-可见分光光度法测定6批氯霉素炉甘石中氧化锌的含量。结果：在氧化锌含量在0.516-5.160μg/mL范围内,线性关系良好,回归方程为：Y=0.2743X-0.0444（r=0.9962）,氯霉素炉甘石中氧化锌的平均含量为11.42g/瓶（RSD=3.86%）,结论：与络合滴定法相比,该法准确、灵敏度高、重复性好,而且简便快捷,适用于氯霉素炉甘石中氧化锌含量的质量控制。     

氯霉素甲硝唑洗鼻剂的含量测定及其有关物质检查方法

目的建立高效液相色谱法测定氯霉素甲硝唑洗鼻剂中氯霉素、甲硝唑含量测定方法及其有关物质氯霉素二醇物、对硝基苯甲醛的定量检查方法。方法采用高效液相色谱法。Intersil C18分析色谱柱(4.6mm ID×250mm,粒径5μm),流动相:甲醇-水-三乙胺(480∶520∶7,含1%磷酸二氢钠,0.2%庚烷磺酸钠,磷酸调pH值为3.0);流速1.0ml/min;检测波长270nm。结果甲硝唑、氯霉素、氯霉素二醇物和对硝基苯甲醛的理论板数分别为4 700、4 5004、400、1 500。回归方程分别为:Y=19156 100.5X+424 145.7(r=0.999 9),Y=65 390 024.2X-5 660.6(r=0.999 9),Y=39 817 070.9X+1 373 589.3(r=0.999 8),Y=83 051 977.5X-284 191.7(r=0.999 8);线性范围分别为0.423 0~2.115μg,0.450 0~2.250μg,0.660 0~3.300μg,0.444 0~2.220μg;平均回收率和RSD分别为100.8%,1.4%;99.2%,1.2;101.1%,1.5;99.3%,1.6;最低检测限分别为0.002,0.002,0.002和0.003μg。结论用高效液相色谱法测定氯霉素甲硝唑洗鼻剂中甲硝唑、氯霉素二醇物、氯霉素、对硝基苯甲醛的含量,操作简便,结果准确。     

利用单纯形法对氯霉素及其分解产物GLC分离条件的优化

本文以色谱响应函数CRF_(RT)作为色谱分离性能的参考尺度,利用单纯形法对氯霉素及其分解产物的GLC分析条件进行了优化。通过9次试验,找到了以2%SE-30 Chromosrb W Aw-Dmcs 80-100目为固定相时满意的分离条件:柱温为245℃,载气流速为56ml/min。在保证峰的良好分离情况下使分析时间由原来的13.5分缩短至5.09分。本实验表明单纯形法是药物色谱分离最优化中一种简单有效的方法。     

DNA transfection in SK-N-BE(2)C human neuroblastoma cells

DNA transfection conditions were investigated by calcium phosphate-DNA co-precipitation in SK-N-BE(2)C human neuroblastoma cells. The DNA plasmid of TH2400CAT was used in which rat tyrosine hydroxylase gene was inserted into chloramphenicol acetyltransferase reporter gene. The transfection efficiency was 25≈30% and the method was simple and reproducible. So, the method will be a good tool for transient transfection analysis.     

Comparison of the determination of chloramphenicol residues in aquaculture tissues by time-resolved fluoroimmunoassay and with liquid chromatography and tandem mass spectrometry

A competitive time-resolved fluoroimmunoassay (TR-FIA) was developed for the determination of chloramphenicol (CAP) residues in aquaculture tissues based on monoclonal antibodies. The limits of detection (LOD) were determined to be 0.04 ng g^-1 and the limits of quantification (LOQ) were less than 0.15 ng g^-1. The intra-assay variations were below 10% and the interassay variations ranged between 9.7 and 13.3%. The mean recoveries established at six concentration levels varied from 83.7-109.6% and the coefficient of variation was from 8.3-11.5%. The results obtained by the TR-FIA and ELISA showed a good correlation. The established TR-FIA was validated for the determination of incurred aquaculture tissues and confirmed by high-performance liquid chromatography and tandem mass spectrometry (LC-MS-MS). This proposed technique could be applied to routine residue analysis.     

The Distribution and Fate of 14C-Chloramphenicol in the New-born Pig

Abstract: The distribution of ¹⁴C-chloramphenicol has been studied in new-born pigs with the aid of whole-body autoradiography. In the lung, liver, adrenal cortex, kidney, myocardium, pancreas, thyroid, spleen and skeletal muscle the amounts of radioactivity were higher than that of the blood short time after injection and remained higher than the blood up to 8 hrs. After 4 and 8 hrs the brain concentration of ¹⁴C was also higher than that of the blood. In the bone marrow, however, the concentration did not reach that of the blood during the whole experiment. In the organs more than 90% of the radioactivity was represented by chloramphenicol; the excretory organs, thyroids, and adrenals being exceptions.     

The lethal effects of delta9-tetrahydrocannabinol in mice enhanced by pretreatment with SKF 525-A or chloramphenicol

The i.p. lethal activity of THC following single dose administration was significantly enhanced by pretreatment with SKF 523-A (30.0 mg/kg) or chloramphenicol (100.0 mg/kg) as evidenced by a significant reduction in the LD₅₀ value. Moreover, the onset to lethality was also sharply reduced. These results are discussed in light of the present knowledge concenring the metabolism of THC.     

HPLC法测定复方地塞米松滴耳液中二组分的含量

目的建立HPLC法测定复方地塞米松滴耳液中二组分的含量。方法采用Waters XTerraRP18（250mm×4.6mm,5μm）,以甲醇-水（70∶30）为流动相,流量为1.0mL.min-1,柱温为30℃,检测波长240nm。结果本色谱条件下醋酸地塞米松和氯霉素分离良好。醋酸地塞米松、氯霉素分别在0.002～0.05mg.mL-1和0.02～0.5mg.mL-1浓度范围内,与峰面积呈良好的相关性（r=1.0000和r=0.9999）;平均回收率：醋酸地塞米松为100.3%,RSD为0.7%（n=9）,氯霉素为100.0%,RSD为0.4%（n=9）。结论该方法简便、准确、可靠,适用于复方地塞米松滴耳液的定量检测。     

康复新液加减治疗128例痤疮疗效分析

目的评估康复新液治疗痤疮的临床疗效及安全性。方法采用8周康复新液加减对照2％氯霉素酊加减治疗寻常型、脓疱型痤疮,根据自觉症状、皮损及痘印消褪程度测评康复新液疗效。结果康复新液组128例在治疗8周,随访12周,疗效明显优于对照组,差异有统计学意义（P〈O．05）。结论康复新液组冷湿敷治疗面部痤疮疗效显著,无不良反应,值得临床推广应用。