乙型病毒性肝炎患者血清AngⅡ与HA的相关性研究

目的探讨乙型病毒性肝炎(简称乙肝)患者的血清血管紧张素Ⅱ(AngⅡ)与透明质酸酶(HA)之间的关系。方法选取2013年2月-2014年2月海南省人民医院收治的乙肝患者300例,其中急性乙肝患者142例(急性组),慢性乙肝患者158例(慢性组),选择同期体检的健康人群100名作为对照组。采用速率法检测血清AngⅡ水平,电化学发光法检测HA水平,对三组的结果进行统计分析。结果慢性组血清AngⅡ和HA水平明显高于急性组和对照组,急性组明显高于对照组,差异有统计学意义(t=1.306、2.839、1.804,P均0.05);Pearson相关系数分析显示:血清AngⅡ和HA水平呈正相关(r=0.703,P0.05)。结论乙肝患者血清AngⅡ和HA水平关系密切,两者均参与了肝纤维化进程,可作为乙肝患者肝纤维化程度的判定指标。     

人精子细胞膜透明质酸酶对肾上腺癌细胞SW13增殖影响的研究

目的　探讨人精子细胞膜结合型透明质酸酶 (PH2 0 )对人肾上腺癌细胞SW 13生长的影响。方法将人全长PH2 0cDNA转染到体外培养人肾上腺癌细胞SW 13细胞株中 ,将细胞置入软琼脂培养板中 ,研究集落形成的改变 ;再将同样的细胞种植到 10d龄鸡胚绒毛尿囊膜 (CAM )上 ,研究肿瘤生长大小的变化 ;细胞培养在2 4孔培养板中 ,采用细胞计数方法研究细胞的生长曲线。结果　转PH2 0基因的SW 13细胞在软琼脂培养板中的集落形成能力明显增强 ,CAM上的SW 13增殖程度明显高于对照组 ,转PH2 0基因后SW 13细胞生长曲线上升。结论　PH2 0可促进人肾上腺癌细胞SW 13的增殖 ,提高SW 13细胞的恶性度     

透明质酸酶对慢性压迫性神经损伤模型中神经源性疼痛的作用研究

目的:研究透明质酸酶对坐骨神经的疏松结扎(CCI)模型神经源性疼痛的作用,初步探讨临床通过相关穴位点注射透明质酸酶治疗慢性疼痛的机制。方法:采用CCI模型,记录损伤神经异位自发放电和热痛刺激的敏感程度。结果:用透明质酸酶作用于术后7d大鼠的损伤处并进行坐骨神经自发放电的测量,发现在测量的12只模型中,有8只模型的自发放电得到了有效的抑制,这8只的平均抑制率为50.9%±12.4%。在热刺激行为学实验中,损伤局部持续注射透明质酸酶溶液的CCI大鼠在术后3d、6d和14d的热刺激敏感度明显低于局部注射生理盐水的对照组CCI大鼠。通过CCI大鼠损伤坐骨神经组织切片观察,发现神经纤维排列混乱,纤维间存在空泡状间隙,并且在间隙周围分布着染成深色的多糖链;而经过透明质酸酶作用后CCI损伤坐骨神经切片观察,空泡状空隙与深色多糖链沉淀消失,神经排布较处理前更加规则。结论:细胞外基质与神经生理功能密切相关,透明质酸酶能够有效降低CCI模型的异位自发放电;临床穴位注射透明质酸酶改变穴位点聚集的细胞外基质,从而起到镇痛作用。     

HYAL-1 Hyaluronidase: A Potential Prognostic Indicator for Progression to Muscle Invasion and Recurrence in Bladder Cancer

Background

For bladder cancer (BCa) patients undergoing bladder-sparing treatments, molecular markers may aid in accurately predicting progression to muscle invasion and recurrence. Hyaluronic acid (HA) is a glycosaminoglycan that promotes tumor metastasis. Hyaluronoglucosaminidase 1 (HYAL-1)–type hyaluronidase (HAase) promotes tumor growth, invasion, and angiogenesis. Urinary HA and HAase levels are diagnostic markers for BCa.

Objective

We evaluated whether HA and HYAL-1 can predict progression to muscle invasion and recurrence among patients with non–muscle-invasive BCa.

Design, setting, and participants

: Based on tissue availability, tissue microarrays were prepared from a cohort of 178 BCa specimens (144 non–muscle invasive, 34 muscle invasive). Follow-up information was available on 111 patients with non–muscle-invasive BCa (mean follow-up: 69.5 mo); 58 patients recurred and 25 progressed to muscle invasion (mean time to progress: 22.3 mo).

Measurements

HA and HYAL-1 expression was evaluated by immunohistochemistry and graded for intensity and area of staining. Association of HA and HYAL-1 staining with BCa recurrence and muscle invasion was evaluated by univariate and multivariate models.

Results and limitations

HA and HYAL-1 expression correlated with tumor grade, stage, and multifocality (p < 0.05). In non–muscle-invasive BCa specimens, HYAL-1 staining was higher (234.3 ± 52.2; 200.6 ± 61.4) if patients experienced progression to muscle invasion or recurrence when compared with no progression or recurrence (164.1 ± 48.2; 172.1 ± 57; p < 0.001). HA staining correlated with muscle invasion (p < 0.001). In univariate analysis, age (p = 0.014), multifocality (p = 0.023), and HYAL-1 staining (p < 0.001) correlated with muscle invasion, whereas only HYAL-1 correlated with recurrence (p = 0.013). In multivariate analysis, HYAL-1 significantly associated with muscle invasion (p < 0.001; 76.8% accuracy) and recurrence (p = 0.01; 67.8% accuracy).

Conclusions

HYAL-1 is a potential prognostic marker for predicting progression to muscle invasion and recurrence.     

Does the addition of hyaluronidase improve the quality of peribulbar anesthesia in cataract surgery? – A randomized double blinded study

Purpose

To determine the necessity of hyaluronidase as an anesthetic adjuvant for peribulbar anesthesia during cataract surgery and to assess differences in anesthetic outcomes in the absence of hyaluronidase.

卵巢肿瘤患者血清中透明质酸酶活性及透明质酸含量的测定

目的 :检测卵巢肿瘤患者血清中透明质酸酶活性及透明质酸含量 ,评价它们在卵巢恶性肿瘤诊断、术后监测中的应用价值及与临床病理参数间的关系。方法 :使用ELISA like方法检测 36例恶性卵巢肿瘤术前血清 ,34例术后随访血清 (包括复发组 14例 ,未复发组 2 0例 ) ,2 0例良性卵巢肿瘤血清及 10例正常对照血清中透明质酸酶活性及透明质酸含量。结果 :恶性卵巢肿瘤患者治疗前血清透明质酸酶活性高于良性肿瘤及正常对照组 (P <0 .0 1) ;血清透明质酸酶活性与临床病理参数无相关性 ;随访血清透明质酸酶活性复发组较未复发组升高 (P <0 .0 5 )。以 30 0U/L为界值 ,检测恶性卵巢肿瘤的灵敏度为81.5 % ,特异度为 77.3% ,而监测临床复发的灵敏度为 85 .7% ,特异度 72 .7%。恶性卵巢肿瘤患者治疗前血清透明质酸含量高于良性肿瘤及正常对照组 (P <0 .0 1) ,且与FIGO分期、病理类型、腹水细胞学相关 ,FIGOⅢ～Ⅳ期高于Ⅰ～Ⅱ期 (P <0 .0 5 ) ,上皮性肿瘤高于其他类型 (P <0 .0 5 ) ,腹水细胞学阳性组高于腹水细胞学阴性者 (P <0 .0 5 ) ;随访血清透明质酸含量复发组高于未复发组 (P <0 .0 1)。血清透明质酸酶活性与透明质酸含量无相关性。结论 :透明质酸酶活性检测对诊断恶性卵巢肿瘤及检测复发有重要意义 ,透明质酸、?     

微创引流术治疗脑室出血并鞘内注射药物预防脑积水

目的观察微创引流术治疗脑室出血并鞘内注射(鞘注)药物预防脑积水的疗效。方法采用微创引流术并鞘注药物的方法治疗脑室出血7例，观察其疗效。结果本组病人意识恢复，颅内压下降，脑脊液转清均较好，且无脑积水并发症。结论微创引流术并鞘注药物是治疗脑室出血和预防脑积水的有效方法。     

透明质酸、透明质酸酶和RGD抑制胃癌细胞SGC7901黏附侵袭的实验研究

目的　探讨单独及联合应用透明质酸 (HA)、透明质酸酶 (Hase)和RGD对SGC790 1细胞黏附和侵袭细胞外基质 (ECM)的抑制作用。方法　采用间接免疫荧光法测定SGC790 1细胞表面CD4 4与integrinβ1蛋白表达。单独或联合应用HA、Hase和RGD处理体外培养的胃癌细胞SGC790 1;MTT法和Boyden小室模型分别测定其黏附力和侵袭力 ,同时观察细胞形态。结果　人胃癌细胞系SGC790 1表达CD4 4与integrinβ1蛋白。与对照组比较 ,Hase及RGD肽均明显抑制SGC790 1的黏附力(P <0 .0 0 1)和侵袭力 (P <0 .0 5 ) ,效果优于HA(P <0 .0 5 ) ;Hase HA或三者联合的抑制效果优于任何单一抑制效果 (P <0 .0 0 1)。形态学观察发现 ,未处理组的部分细胞已经开始铺展 ,穿过人工基底膜后 ,表现出纤维母细胞样形态及形态各异的伪足 ;而处理组细胞形态较圆 ,伪足数目相对较少。结论　人胃癌细胞系SGC790 1表达功能性CD4 4和integrinβ1蛋白。单独及联合应用HA、Hase和RGD均不同程度抑制SGC790 1细胞对ECM的黏附和侵袭 ,以联合三者阻断效果为优。     

Allergic reaction to hyaluronidase: a rare cause of orbital inflammation after cataract surgery

Purpose The aim of this study was to present a series of patients with acute orbital inflammatory symptoms secondary to peribulbar hyaluronidase allergy and to discuss the diagnostic and management modalities.Methods Review of clinical records of all patients with acute orbital inflammatory symptoms after uneventful cataract surgery, seen at two ophthalmology departments. The main outcome measures included clinical signs and symptoms, imaging findings, post-operative course, skin allergy testing, and final outcome.Results There were five patients (four women, one man) with a mean age of 78±7.0 years. Signs and symptoms appeared 12–72 h after cataract surgery and included axial proptosis, periorbital erythema with swelling, and extraocular muscle (EOM) function restriction (5/5), periorbital pain or itchiness (3/5), and conjunctival chemosis (4/5). Computerized tomography showed increased orbital fat haziness and enlargement of EOM. Treatment with a combination of oral antibiotics and steroids or antihistamines resulted in resolution of signs and symptoms after 3–5 days. Intradermal and/or skin prick allergy tests were positive for hyaluronidase and negative for all other perioperative medications used.Conclusion Although uncommon, allergy to peribulbar hyaluronidase injected during cataract surgery should be considered in the differential diagnosis of patients who present with acute post-operative orbital signs and symptoms.     

Optimization of Physical and Nutritional Parameters for Hyaluronidase Production by Streptococcus mitis

The effect of some physical and nutritional parameters were studied for the optimum production of extracellular enzyme hyaluronidase employing Streptococcus mitis MTCC*2695 by submerged fermentation. The effects of initial pH, incubation temperature and time, inoculum level and age of inoculum were studied. The maximum enzymatic activity was obtained with an initial pH 5.8, incubation temperature 37°, incubation time for 48 h and inoculum level 6% with inoculum age 24 h. The effect of different carbon and nitrogen sources and antibiotics were studied. The results indicated that sucrose and ammonium chloride showed the highest enzymatic activity among various carbon and nitrogen sources. Antibiotic clarithromycin showed strong inhibitory effect on hyaluronidase production.