核仁组成区嗜银蛋白染色在非霍奇金淋巴瘤骨髓涂片中的价值探讨

目的：探讨核仁组成区嗜银蛋白染色在非霍奇金淋巴瘤骨髓涂片中的价值。方法用经钟氏等报道[1]的双层滤纸垫染法对117例NHL骨髓涂片进行染色后检测,并另做17例大致正常骨髓片做对照。结果 NHL组、NHL晚期组二者的银染颗粒含量和银染颗粒阳性率均较正常对照组有显著性差异（两组P〈0.05）;NHL晚期组的银染颗粒含量和银染颗粒阳性率均较NHL早期组有显著性差异（两者P〈0.05）;而NHL早期组的银染颗粒含量和银染颗粒阳性率均与正常对照组无显著性差异（P均〉0.05）。结论 AgNOR染色在NHL骨髓涂片的诊断中具有较大的应用价值。     

内镜诊断技术在Barrett食管中的应用及进展

Barrett食管(BE)诊断主要依赖于内镜及组织病理学检查。提高BE的诊断水平,为及早治疗及干预其向食管腺癌的发展具有重要的意义。近年来,BE诊断技术得到进一步发展,普通内镜、胶囊内镜、染色内镜、染色放大内镜、增强放大内镜、荧光内镜技术、超声内镜、共聚焦荧光内镜、窄带光照内镜技术在诊断BE中具有不同特点。现对BE诊断标准、不同内镜诊断技术特点进行综述。     

The effect of time on grading corneal fluorescein and conjunctival lissamine green staining

PurposeTo explore the effect of time on grading corneal fluorescein and conjunctival lissamine green staining in dry eye disease (DED).MethodsPhotographs of 68 subjects with non-Sjogren's DED (nSS DED) and 32 with Sjogren's DED (SS DED) were taken of corneal fluorescein staining, then conjunctival lissamine green staining every 30 s for at least 5 min. Photographs of one randomly selected eye were then randomly ordered and graded on a scale from 0 to 5 (severe staining) by two clinicians, masked to both site and subject. The average time required to reach the maximum grade of staining (Gmax) was calculated.ResultsThe median time (upper and lower quartiles) to corneal fluorescein Gmax was 2.6 (1.3–5.3) minutes for nSS DED and 3.8 (2.6–5.4) minutes for SS DED, a statistically significant difference (Mann Whitney U test, p = 0.018). In contrast, the median time to the Gmax for lissamine green staining of the nasal and temporal conjunctiva was 0.5 (0.5–1.1 nasal, 0.5–0.8 temporal) minutes for nSS DED and 0.5 (0.5–0.8 nasal, 0.5–0.5 temporal) minutes for SS DED subjects, which was not statistically significant (p ≥ 0.383).ConclusionsThe time required to reach the maximum grade of corneal fluorescein staining, but not conjunctival lissamine green staining, varied widely and was significantly longer in subjects with Sjögren's Syndrome. Early observation of corneal fluorescein staining can lead to under-grading, which may impact the diagnosis and assessment of treatment in DED. Further study of the best time to assess corneal fluorescein staining in various DED populations is warranted.     

Evaluation of natural dyes for human spermatozoa morphology assessment

Dye extracts from plants have been valuable not only for the economy but also environmental sustainability. There have been many reports on the utilization of natural dyes extracted from various sources for staining of biological tissues. This study aimed to investigate the extraction of natural dye from black rice (Oryza Sativa), butterfly pea (Clitoria ternatea), fresh roselle (Hibiscus sabdariffa), and mulberry (Morus alba) to stain of human spermatozoa for morphology assessment. The results showed that black rice extracted from solvents C containing 5?ml of absolute ethanol, 10?g of potassium alum and 100?ml of distilled water is the best dye for human spermatozoa evaluation comparable to the rapid PAP and Dip quick® stain. Effectiveness of the process was found with black rice extract stain by using 2 steps for 15?min. There were no statistically significant differences in the parameters of head, midpiece, tail and background for human sperm morphology assessment comparable to rapid PAP and Dip quick® stain (p?>?0.05) unless the midpiece compartment when compare to rapid PAP. This finding suggests that the black rice extracted has potential for use as an alternative dye for human spermatozoa morphology evaluation. The usefulness of black rice extracts will decrease the expense for purchasing synthetic dyes and reduce their adverse effects on human and environment.     

新型超顺磁性氧化铁标记SD大鼠脂肪干细胞的有效性及安全性研究

目的：采用新型超顺磁性氧化铁对 SD 大鼠来源的脂肪干细胞（ADSCs）进行标记,并与既往商用 SPIO 标记效果进行对比,探讨这种新型超顺磁性氧化铁标记的有效性及安全性。方法：分离、纯化、鉴定 SD 大鼠来源的 ADSCs,然后分不同浓度组（0、6、12、25、50和100μg／mL）和时间组（6、12、24和48 h）进行标记,通过普鲁士蓝染色测定铁标记率;在不影响细胞形态的前提下,对达到95％以上铁染色率的孵育浓度、时间进行标记安全性检测,包括活力、增殖力、细胞表面抗原表达;采用透射电子显微镜观察标记细胞的超微结构,采用 ICP-AES 对标记细胞内的铁含量进行测定,并与商用SPIO 标记效果进行对比。结果：在无细胞毒性的前提下,新型 SPIO 达到95％以上铁染色率的孵育浓度是12和25μg／mL,孵育时间是12 h;ICP-AES 检测显示具有表面正电荷的聚乙二醇（PEG）／聚乙烯亚胺（PEI）修饰的 SPIO 标记后细胞内的铁含量达到35．4 pg／cell（25μg／mL 中孵育12h 后）和20．16 pg／cell（12μg／mL 中孵育12h 后）,并随着孵育浓度的增加,细胞内的铁含量增加;而具有表面零电荷的 PEG／聚乙烯吡咯烷酮（PVP）修饰的 SPIO 标记后的铁含量仅为6．96 pg／cell（25μg／mL 中孵育12h 后）;透射电子显微镜显示标记后细胞器结构完整,内吸收的 SPIO 主要位于细胞质内的囊泡和溶酶体中。结论：新型 SPIO 在适当孵育浓度和时间下可以安全、快速标记 ADSCs;PEG／PEI 修饰的 SPIO 标记效果要远远比既往商用的 SPIO 快速有效,可作为一种优势的新型磁性标记物用于干细胞标记;而 PEG／PVP 修饰的SPIO 比起既往商用的 SPIO 并无明显优势,说明表面电荷在细胞标记中占有极其重要的角色。     

免疫酶染色法检测人血清中抗幽门螺杆菌抗体的效果评价及应用

幽门螺杆菌与胃炎、消化性溃疡有着非常密切的关系,但诊断方法仍以胃镜采样然后进行培养涂片、尿素酶试验为主;或使用需要一定设备的ＥＬＩＳＡ方法进行检测,难以在一般有消化系统症状的患者中使用,给Ｈ．ｐ感染的诊断和治疗带来了困难。本文对不需特殊设备的ＩＥＳＴ用培养涂片和ＩＥＬＩＳＡ方法进行了比较和应用。我们应用免疫酶染色法（ＩＥＳＴ）对９０名作胃镜检查者血清中抗幽门螺杆菌（Ｈ．ｐ）抗体ＩｇＧ进行了检测。其Ｈ,ｐ感染的检出率为７４．４％,高于细菌培养和镜检的检出率。灵敏度为９４．９％,特异度为６４．５％。各项指标均优于ＩＥＬＩＳＡ方法。在ＩＥＳＴ抑制试验中,幽门螺杆菌与大肠杆菌空肠弯曲等无明显交叉反应,显示了其特异性,同时该法简便,经济,不需特殊仪器且易被受检者接受,值得推广应用。     

Ehrlich苏木精染液pH值变化对组织染色的影响

目的 探讨Ehrlich苏木精染液的pH值变化对组织染色的影响,寻求提高HE染色质量的方法.方法 用2 mol/L NaOH和HCl分别处理Ehrlich苏木精染液,测定其pH值;观察组织切片在不同pH值苏木精染液中的染色效果.结果 组织切片在低pH值染液中细胞核着色清楚,呈蓝色或深蓝色,核浆对比清晰,无苏木精沉渣附着...     

Composite resin color stability: influence of light sources and immersion media

Objective

This study evaluated the influence of light sources and immersion media on the color stability of a nanofilled composite resin.

Material and Methods

Conventional halogen, high-power-density halogen and high-power-density light-emitting diode (LED) units were used. There were 4 immersion media: coffee, tea, Coke^® and artificial saliva. A total of 180 specimens (10 mm x 2 mm) were prepared, immersed in artificial saliva for 24 h at 37±1ºC, and had their initial color measured with a spectrophotometer according to the CIELab system. Then, the specimens were immersed in the 4 media during 60 days. Data from the color change and luminosity were collected and subjected to statistical analysis by the Kruskall-Wallis test (p<0.05). For immersion time, the data were subjected to two-way ANOVA test and Fisher''s test (p<0.05).

Results

High-power-density LED (∆E=1.91) promoted similar color stability of the composite resin to that of the tested halogen curing units (Jet Lite 4000 plus - ∆E=2.05; XL 3000 - ∆E=2.28). Coffee (∆E=8.40; ∆L=-5.21) showed the highest influence on color stability of the studied composite resin.

Conclusion

There was no significant difference in color stability regardless of the light sources, and coffee was the immersion medium that promoted the highest color changes on the tested composite resin.     

阴道分泌物常规检验室内质量控制结果分析

目的：分析阴道分泌物（白带）常规检验室内质量控制（IQC）效果。方法：收集2011年1月—12月到贵州省人民医院妇产科就诊患者白带标本1 235份,每天抽取5份悬浮法检测的白带标本,涂片用瑞氏染色镜检,可疑淋球菌时加革兰染色复检,以此对悬浮法检测结果进行质量考核。每月不定期采用直接镜检及离心沉渣涂片镜检抽查生理盐水质量。结果：2011年的12个月中,仅7月和9月的线索细胞的符合率不合格,分别为74.3%和78.1%,其他月份的各项目检测符合率均达到合格标准,即82.5%～100%,月平均90.2%～94.7%,为优良等次;病原体中滴虫的平均符合率最高（99.3%）,线索细胞的平均符合率最低（83.1%）。生理盐水质量为合格。结论：采用瑞氏法为主的复查监督措施,建立的悬浮法白带常规检查IQC方法可有效保证其检验质量。