中医药不同时机介入治疗脑梗死急性期

目的探讨中医药对脑梗死急性期患者不同时机介入治疗的疗效。方法在脑梗死急性期患者常规治疗的基础上,采用中医药介入疗法,时间点选择在治疗的第1、3、5、7天,利用美国国立卫生研究院卒中量表及日常生活活动量表(Brathel指数)等方法分析其临床疗效。结果Ⅲ组(第3天)介入中医药治疗总有效率为95.0%,优于其他各组。结论第3天介入中医药治疗效果最为明显,是相对平衡的介入时间点。     

WWOX基因对人急性淋巴细胞性白血病细胞CCRF-CEM细胞凋亡及细胞周期的影响

目的:研究WWOX基因对人急性淋巴细胞性白血病细胞CCRF-CEM细胞凋亡及细胞周期的影响及分子机制。方法:体外培养CCRF-CRM细胞系，将WWOX基因转染入CCRF-CEM细胞，建立高表达WWOX的CCRF-CEM细胞，流式细胞仪法测定CCRF-CEM细胞的细胞周期和凋亡，Western blot法测定WWOX基因、细胞周期和凋亡相关因子的蛋白表达，qRT-PCR法测定caspase-3的mRNA表达。结果:与对照组和空白质粒组相比，转染入WWOX的CCRF-CEM细胞的WWOX蛋白表达显著增加，G0/G1期的细胞比例显著增加，凋亡细胞比例显著增加；CCRF-CEM细胞中cyclin D1、cyclin E、CDK2的蛋白表达显著降低而Wnt-5α和JNK的蛋白表达显著增加，caspase-3的mRNA表达促进CCRF-CEM细胞的凋亡。结论:WWOX基因在急性淋巴细胞性白血病CCRF-CEM细胞中发挥抑癌基因作用，可以有效促进细胞凋亡并抑制细胞周期进程。     

Cell attenuated porcine epidemic diarrhea virus strain Zhejiang08 provides effective immune protection attributed to dendritic cell stimulation

Since 2010, the porcine epidemic diarrhea coronavirus (PEDV) has caused significant damage to the global pork industry. However, classical PEDV vaccine strains only provide limited protection against emerging strains. In this study, we successfully isolated and attenuated the PEDV epidemic strain Zhejiang08, which was characterized by good cell adaptation and high-titer production 48 h post infection in Vero E6 cells. The attenuated virus induced a high level of virus-specific neutralizing antibodies until 120 days after immunization in piglets and provided complete protection when challenged with an emerging virus strain on day 14 post immunization. Moreover, the capability to activate dendritic cells (DCs) of this isolate was identified. Higher expression levels of IL-12 and IFN-γ were recorded in DCs after treatment with Zhejiang08 for 24 h. Furthermore, genome sequencing and phylogenetic analysis revealed high homology between the main antigen epitopes of Zhejiang08 and PEDV pandemic isolates following 2011. Combining the glycosylation site prediction results and their distribution within the spatial structure of the S protein, led to the conclusion that the observed more effective host immune response of Zhejiang08 compared to CV777 was possibly associated with a lack of the potential glycosylation site in the 296 amino acids of the S protein. In summary, we illustrated that the attenuated virus represents a promising vaccine candidate.     

连续性血液净化对急性呼吸窘迫综合征单核细胞分泌功能和抗原呈递功能的影响

目的:研究连续性血液净化(CBP)对急性呼吸窘迫综合征(ARDS)患者单核细胞分泌功能和抗原呈递功能的影响.方法:动态检测36例接受CBP的ARDS患者单核细胞功能,并与健康人群进行比较.结果:ARDS患者单核细胞分泌细胞因子功能高于健康人群,而抗原呈递功能与健康人群相比无显著性差异.CBP治疗48h后,单核细胞细胞分泌功能恢复至正常水平,CBP治疗对单核细胞抗原呈递功能无显著性影响.结论:CBP治疗可降低ARDS单核细胞的细胞因子分泌功能,但对其抗原呈递功能无明显影响.     

Acute hepatitis B in Japan: Incidence,clinical practices and health policy

Aim: The epidemiology of acute hepatitis B is unknown in many countries, and the clinical features of this disease remain unclear. In this study, we used the Diagnosis Procedure Combination (DPC) database to estimate the incidence of acute hepatitis B and investigate the clinical practices for acute hepatitis B in Japan. Methods: The DPC database is a nationwide discharge abstract and administrative claims database, covering 40% of all inpatient admissions to acute care hospitals between 1 July and 31 December each year in Japan. We identified cases with a diagnosis of acute hepatitis B between 2007 and 2008. Patient characteristics, length of stay, in‐hospital mortality and total charges were determined. Clinical practice patterns were examined, including drugs used and procedures performed during hospitalization. Results: We identified 890 cases with acute hepatitis B among 5.85 million inpatients in the database. The mean age was 40.0 years old and 76% were male. The incidence of acute hepatitis B was estimated to be approximately 2100–2400/year (17–19/1 million people per year). Of 890 cases, 53 (6.0%) developed fulminant hepatitis and 36 (4.0%) died. Nucleos(t)ide analogs were prescribed for 226 cases (25.4%). Only 194 cases (21.8%) were tested for HIV status. Conclusion: It is essential to monitor the trends of this communicable and preventable disease. The establishment and distribution of appropriate clinical evidence and guidelines are vital to improve the clinical practices for acute hepatitis B.