屋面分部工程防水保温施工的组织设计

医院作为特殊的公共建筑,除满足公共建筑的共性外,还具有自身特殊的个性要求。因此对屋面分部工程防水保温施工工程上选用材料及施工要求会有更加严格的要求。     

精卵孵育时间对体外受精-胚胎移植结局的影响

[目的]比较体外受精-胚胎移植中精卵孵育不同时间(4～6h和16～18h)对体外受精-胚胎移植结局的影响。[方法]272例体外受精-胚胎移植(IVF-ET)周期根据精卵孵育不同时间分为两组:A组:199例采集的卵子,在加精后4～6h取出放入新鲜培养液中培养;B组:73例采集的卵子,加精过夜培养后(16～18h)取出放入同种培养环境中培养。比较两组的受精率(包括正常受精率和异常受精率)、卵裂率、优质胚胎率、临床妊娠率以及胚胎种植率。[结果]A组受精率明显高于B组,差异有统计学意义(P﹤0.01);A组正常受精率及优胚率高于B组,差异有统计学意义(P﹤0.05);两组的卵裂率、临床妊娠率及胚胎种植率的差异均无统计学意义。[结论]缩短精卵孵育时间能够提高正常受精率和胚胎的质量,从而提高胚胎的种植潜能。     

加样反应温育条件对ELISA法检测丙肝抗原的影响

目的：探讨丙肝抗原检测（ELISA法）加样后改变反应温度及时间对检测结果的影响。方法：对234例样本分别在37℃温育90min及在43℃温育45min的条件下检测丙型肝炎病毒核心抗原（HCV-cAg）的S/CO值。结果：两种温育条件对于样本阳性检出率、吸光度值、S/CO值没有显著影响。结论：适当地提高温育温度及缩短温育对HCV-cAg的检测结果影响不明显。这有助于缩短临床检验工作者的工作时间,尽快报告结果,提高工作效率。     

RESIDUAL ACETYLCHOLINESTERASE ACTIVITY IN THE BASAL LATERAL AMYGDALA FOLLOWING SOMAN-INDUCED STATUS EPILEPTICUS

Soman (pinacolylmethylphosphonofluoridate) causes seizures and seizurerelated brain damageresulting from theirreversibleinhibition of acetylcholinesterase (AChE) in the central nervous system (CNS). The authors have examined the possibility that the loss of AChE reactivity in discrete brain regions may have importance in the development of status epilepticus caused by soman. In these studies, male Sprague-Dawley rats were given GM monosialoganglio1 side, intracerebroventricularly, via an osmotic minipump. Four days after initiation of GM1 infusions, rats were injected with 1.25 LD50 soman (83mug/kg,im). Electrocorticogram (ECoG) recordings were monitored via indwelling cortical electrodes. All rats were euthanized 27 h after soman administration. Brain tissue was sucrose-saturated, cryostat-sectioned, and processed for AChE histochemistry. A Quantimet 600 Image Analysis System and Biological Microscope were used to assess the optical density of histochemical staining for AChE in the piriform cortex, basolateral amygdaloid nucleus, and caudate putamen. All rats showed ECoG evidence of seizures within 10 min of soman administration. However, 6 of the 11 surviving rats receiving GM1 infusion did not develop status epilepticus. Statistical analysis of AChE optical densities revealed marked and significant reductions of AChE reactivity in soman-treated rats compared to controls. Soman-treated rats that did not develop status epilepticus had significant levels of residual AChE in the basolateral amygdaloid nucleus compared to other soman-challenged animals.     

潜伏期行椎管内阻滞麻醉分娩镇痛对孕妇分娩结局及 新生儿预后的影响

目的 分析潜伏期行椎管内阻滞麻醉分娩镇痛对孕妇分娩结局及新生儿预后的影响。方法 选取本院自2016年12月~2018年12月收治的120例产妇作为研究对象,以随机数字表法分为对照组和研究组,各60例。对照组活跃期行椎管内阻滞麻醉,研究组潜伏期行椎管内阻滞麻醉,比较产程、VAS评分、阴道自然分娩率、新生儿窒息率。结果 研究组第一、第二产程时间为（8.12±0.62）h、（1.01±0.25）h,分别短于对照组的（9.95±1.02）h、（1.48±0.31）h,差异有统计学意义（P＜0.05）,研究组镇痛5 min、30 min、60 min后VAS评分分别是（5.58±0.62）分、（3.25±0.31）分、（0.86±0.12）分,低于对照组的（5.31±0.41）分、（3.98±0.41）分、（1.16±0.18）分,差异有统计学意义（P＜0.05）。研究组阴道分娩率（86.67%）高于对照组（66.67%）,新生儿窒息率（3.33%）低于对照组（20.00%）,差异均有统计学意义（P＜0.05）。结论 潜伏期行椎管内阻滞麻醉进行分娩镇痛,可有效提高阴道自然分娩率,降低新生儿结局,效果显著,值得借鉴。     

1120例药品不良反应/事件的发生特点与转归分析

目的 调查我院药品不良反应/事件(ADR/ADE)发生情况,分析其发生特点和转归,为临床安全、合理用药提供参考.方法 对我院2018年至2019年上报至国家不良反应监测中心的1120例药品不良反应/事件报告进行回顾性分析,探索药品不良反应/事件与年龄、性别、体重、药品种类、给药途径的相关性.结果 在1120例药品不良反...     

抽动障碍之"伏邪致动"学说初探

"感而不发"和"伏而后发"是伏邪致病的特点,临床发现部分抽动障碍患儿发病或复发与外感六淫邪气有一定的病程相关性,与伏邪致病机理相似,故而提出抽动障碍之"伏邪致动"学说,该学说认为外感邪气侵袭肌表,伏藏于半表半里而暂时不发作,在外感邪气、情志变化等刺激下再次发病,病位在肺与肝,与风邪关系密切,外风引动内风,治宜肝肺并调,一则宣肺肃降以疏散外风,二则疏肝通络以熄内风,表里同调,标本兼治。     

Secretion of human chorionic gonadotropin in superfused young placental tissue exposed to cadmium

The effect of various concentrations of cadmium (Cd) in levels ranging from 0.75 to 12 μg/ml medium, on the secretion of human chorionic gonadotropin (hCG) in first-trimester placental expiants, after 6 or 24 h incubation, employing both static and dynamic systems was examined. Later the unbound Cd was washed for 45 min with fresh medium devoid of Cd, followed by superfusion with the latter medium for 75 min, during which time samples were collected for hCG assay. For the superfusion experiments the parameters used for evaluating the hCG secretion pattern were: mean peak amplitude (MPA), pulse frequency (PF) and the area under the hCG secretion curve (AUC). The results indicate that in the dynamic system the hCG secretion increased significantly, and this increase was dose dependent. There was also a dose-related increase in mean total hCG secreted by the explants exposed to Cd. Maximal hCG secretion was observed after 24 h exposure of explants to 6 μg of the metal/ml. Both the MPA and AUC parameters showed a statistically significant increase for this dose level. At 12 μg/ml, the pulsatile secretion of hCG decreased, the value for the mean hCG secretion being comparable to that observed for 0.75 μg/ml. After 6 h incubation, however, there were no significant changes from the control, as judged by all of the above parameters. The levels of hCG secreted by the explants into the media in the static system were not significantly different from their respective controls, for both incubation periods and Cd levels. These results indicate that Cd may affect the normal placental function, as reflected in its hCG secretion pattern.