全文获取类型
收费全文 | 215633篇 |
免费 | 19778篇 |
国内免费 | 8003篇 |
专业分类
耳鼻咽喉 | 1273篇 |
儿科学 | 4806篇 |
妇产科学 | 3033篇 |
基础医学 | 41750篇 |
口腔科学 | 4080篇 |
临床医学 | 13649篇 |
内科学 | 35480篇 |
皮肤病学 | 3616篇 |
神经病学 | 16849篇 |
特种医学 | 3920篇 |
外国民族医学 | 66篇 |
外科学 | 15000篇 |
综合类 | 27674篇 |
现状与发展 | 31篇 |
预防医学 | 9525篇 |
眼科学 | 2512篇 |
药学 | 29754篇 |
31篇 | |
中国医学 | 8291篇 |
肿瘤学 | 22074篇 |
出版年
2024年 | 355篇 |
2023年 | 3153篇 |
2022年 | 4286篇 |
2021年 | 8638篇 |
2020年 | 7524篇 |
2019年 | 8277篇 |
2018年 | 7820篇 |
2017年 | 7996篇 |
2016年 | 7935篇 |
2015年 | 9196篇 |
2014年 | 13399篇 |
2013年 | 15775篇 |
2012年 | 13986篇 |
2011年 | 16307篇 |
2010年 | 13769篇 |
2009年 | 13112篇 |
2008年 | 12217篇 |
2007年 | 10778篇 |
2006年 | 9742篇 |
2005年 | 8231篇 |
2004年 | 7219篇 |
2003年 | 6107篇 |
2002年 | 4722篇 |
2001年 | 3983篇 |
2000年 | 3355篇 |
1999年 | 3013篇 |
1998年 | 2656篇 |
1997年 | 2358篇 |
1996年 | 2074篇 |
1995年 | 1782篇 |
1994年 | 1559篇 |
1993年 | 1297篇 |
1992年 | 1085篇 |
1991年 | 949篇 |
1990年 | 770篇 |
1989年 | 643篇 |
1988年 | 539篇 |
1987年 | 431篇 |
1986年 | 436篇 |
1985年 | 897篇 |
1984年 | 955篇 |
1983年 | 656篇 |
1982年 | 747篇 |
1981年 | 561篇 |
1980年 | 480篇 |
1979年 | 408篇 |
1978年 | 306篇 |
1977年 | 251篇 |
1976年 | 232篇 |
1975年 | 168篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
991.
992.
993.
Hans Knecht Pierre Brousset Edith Bachmann Gorm Pallesen Bernhard F. Odermatt 《Leukemia & lymphoma》1994,15(5):399-403
Two recently discovered genes, the recombination activating genes 1 and 2 (RAG-1 and RAG-2), are necessary to perform variable (V), diversity (D), and joining (J) recombination. They synergistically activate VDJ recombination to generate immunocompetent lymphocytes. Disruption of either gene results in a maturation arrest at a very early B and T cell progenitor stage. Expression and downregulation of RAG's are closely associated with interleukin 7, sIgM and TCR-CD3 complex, respectively. Assessment of RAG mRNA expression is a valuable marker in identifying the genotypic maturation status of leukemias and lymphomas. Persistent RAG expression in otherwise mature lymphoid proliferations may explain puzzling biological and clinical observations such as multiple rearrangements in lymphomas with a mature phenotype. Lack of RAG expression in Hodgkin's disease with abundant Reed-Stern-berg cells is consistent with a mature phenotype of the latter. Availability of a anti-RAG-1 monoclonal antibody in the near future will facilitate RAG analysis of lymphomas. 相似文献
994.
苯DNA加合物研究进展(综述)李桂兰,常平综述徐伯洪审校(中国预防医学科学院劳动卫生与职业病研究所,北京100050)我国职业接触苯的工人约有50万,长期接触苯可引起白血病。苯与其它实质性肿瘤的问题也有报道[1,2]。世界癌症研究机构(IARC)19... 相似文献
995.
996.
L. Szereday p. Varga J. Szekeres-Bartho 《American journal of reproductive immunology (New York, N.Y. : 1989)》1997,38(6):418-422
PROBLEM: In the presence of progesterone lymphocytes of pregnant women release a 34- kDa protein named the progesterone-induced blocking factor (PIBF). PIBF mediates the immunomodulatory and anti-abortive effects of progesterone and its presence is related to the outcome of pregnancy. PIBF induces production of Th2 type cytokines by activated lymphocytes. The in vivo relationship between PIBF- and cytokine production of pregnancy lymphocytes and the outcome of pregnancy was investigated. METHOD OF STUDY: Interleukin (IL)-12 and IL-10 production and PIBF expression in peripheral lymphocytes of 111 healthy pregnant women and 120 women at risk for premature pregnancy termination were detected by immunocytochemistry. RESULTS: We found increased IL-12 and low PIBF and IL-10 expression on lymphocytes of “risk” patients, and a high rate of IL-10 and PIBF positivity on lymphocytes from healthy pregnant women. The cytokine production pattern of the lymphocytes was related to the presence or absence of previous abortions as well as to the outcome of pregnancy. CONCLUSION: These data suggest the involvement of an altered cytokine production pattern in the immunologic effects of progesterone. 相似文献
997.
中国人丙型肝炎病毒囊膜蛋白2HVR1 cDNA的序列分析 总被引:1,自引:0,他引:1
目的:了解中国HCV流行株高变区1(HVR1)的特点。方法:对来自山东(SD)、上海(SH)两地患者血清RNA进行逆转录-巢式PCR,扩增HCV囊膜蛋白2基因片段,用PCR直接测序法对该片段进行序列测定。结果:(1)扩增片段(命名为P388)对应于日本HCV-J株序列核苷酸1439 ̄1826位(氨基酸位371 ̄498);(2)中国人HCV HVR1位于氨基酸位384 ̄410,与发表的HCVⅡ型HV 相似文献
998.
Previous study has shown that α2D-adrenoceptors are involved in modulation of peristalsis in the rat ileum. The aim of the present study was to determine the
tissue location of α-adrenoceptors in the rat ileum by using a recently devised method. The pre-synaptic α-adrenoceptors were
characterised by measuring the potencies of agonists to inhibit transmurally-evoked (1 ms pulses, 10 Hz, 8-10 s trains) contractions
of the longitudinal and circular muscles and the affinities of antagonists. Post synaptic α-adrenoceptors were identified
by screening agonists and antagonists in carbachol-contracted tissues. In the circular muscle the order of potencies for inhibiting
transmurally-induced contraction was: clonidine ≥ oxymetazoline ≥ UK 14,304 ≥ guanfacine > talipexole > phenylephrine > azepexole.
The potency ratios relative to clonidine correlated to those previously derived using the rat ileum peristaltic reflex preparation.
Most of the α-adrenoceptor agonists, however, caused only small inhibitions of the longitudinal muscle contraction in response
to transmural stimulation, except phenylephrine and azepexole. RX 821002, yohimbine, rauwolscine, BRL 44408, phentolamine,
idazoxan, ARC 239, and prazosin inhibited the effect of clonidine on the circular muscle response with apparent pKB values best correlated with pKB or pKi values derived from the rat ileum peristaltic reflex preparation and other tissues known to have the α2D-subtype. The rank order of potencies at inhibiting carbachol-induced responses of both muscle layers was: phenylephrine ≥
oxymetazoline > clonidine ≥ talipexole > azepexole >> guanfacine. UK 14,304 was inactive up to 10 μM. The EC50 value of each agonist on the longitudinal muscle was not significantly different to the corresponding value on the circular
muscle. Prazosin was more potent than yohimbine at inhibiting the relaxant effect of phenylephrine in both muscle layers of
carbachol-contracted tissues. It is concluded that the recently identified α2D-adrenoceptors of the rat ileum are located on cholinergic neurons controlling circular muscle contraction. The study also
demonstrated the presence of postsynaptic α1-adrenoceptors involved in mediating relaxation in both muscle layers.
Received: 4 November 1996 / Accepted: 10 April 1997 相似文献
999.
评价酶联免疫检测血清CYFRA21—1对非小细胞肺癌的诊断价值 总被引:8,自引:0,他引:8
目的评价检测CYFRA21┐1对非小细胞肺癌的诊断价值。方法用ELISA法测定70例肺癌(LC)其中47例非小细胞肺癌(NSCLC),3例小细胞肺癌(SCLC)和20例未分型肺癌、64例肺部良性疾病患者及40例健康人血清CYFRA21┐1浓度。试验的诊断性能用相对操作特征(ROC)分析法估测之。结果测得全阈诊断准确率(OveralDiag┐nosticAccuracies)LC为0.75、NSCLC为0.76,SQC为0.83,ADE为0.67和SCLC为0.38。在相应于特异性为0.95的界定值3.47μg/L处,各型的灵敏度分别为SQC0.62,LC0.53,NSCLC0.51,ADE0.48和SCLC0.00。结论结果显示CYFRA21┐1是NSCLC较灵敏和特异的一个标志物。未观察到TNM各期间该标志物的平均水平有明显的差异;然而异常升高水平的患者的比例随病期的进展而显著增加,提示一系列检测CYFRA21┐1水平可能有助于监查NSCLC患者的疗效。 相似文献
1000.
本研究对123例月经周期正常妇女的N-乙酰-β-D氨基葡萄糖苷酶(简称NAG酶)进行162个周期的观察,使用对硝基苯—N-乙酰-β-D氨基葡萄糖苷作底物与唾液中的NAG酶进行酶促反应。在一定条件下NAG酶作用于底物产生N-乙酰-β-D氨基葡萄糖和对硝基酚,再加一定量的碱溶液终止其酶反应,使对硝基酚呈黄色,然后进行比色,并以尿样LH(单克隆抗体酶免疫法)作为对照观察周期性变化。结果表明:在排卵前和排卵期NAG酶活性上升,通常在下次月经前13~17天之间。准确率90%与尿样LH对照符合率95%。 相似文献