十全大补汤合金铃子散对移植性肝癌大鼠的免疫抑瘤作用

目的:初步研究十全大补汤合金铃子散对移植性肝癌大鼠的免疫抑瘤作用。方法:以大鼠CRL-1548瘤株移植入雄性SD大鼠为模型,系统地观察十全大补汤合金铃子散对瘤重和脾细胞免疫功能的影响。结果:十全大补汤合金铃子散对移植性肝癌大鼠的平均抑瘤率高达51.3%,且剂量依赖性地抑制瘤重量,明显促进NK活性和IL-2分泌,增强腹腔巨噬细胞吞噬能力。结论:十全大补汤合金铃子散增强荷瘤大鼠细胞免疫功能是其抑制肿瘤效应的可能机制。     

分泌型人IL-1β表达载体的构建及在H7402细胞中的表达

目的构建经典途径分泌型人白细胞介素1β(shIL-1β)重组表达载体,检测其在H7402肝癌细胞中的表达。方法采用SOE方法将人红细胞生成素(EPO)信号肽序列和编码人白细胞介素(hIL-1β)成熟蛋白的基因序列拼接形成融合基因,与pIRES2-EGFP质粒重组构建shIL-1β真核表达载体pIRES2-EGFP-shIL-1β,利用jetPEI方法将其稳定转染H7402细胞(H7402／shIL-1β),荧光显微镜及RT-PCR检测融合基因的表达水平,间接ELISA方法测定培养上清hIL-1β分泌水平。结果荧光显微镜下观察可见H7402／shIL-1β细胞发出明亮绿色荧光,RT-PCR检测显示pIRES2-EGFP-shIL-1β能够在H7402细胞中稳定表达融合基因mRNA,同时细胞培养上清中hIL-1β表达水平明显上升。结论成功构建了经典途径分泌的hIL-1β重组表达载体,该载体可在H7402细胞中稳定分泌生物活性的hIL-1β。     

Biological effects of extract from newborn porcine liver on hepatocytes, hepatic stellate cells, and hepatoma cell line

Objective： Porcine liver extract has been shown to be effective in the clinical treatment of severe hepatitis. The aim of the present study was to study its antifibrotic as well as immune regulatory effect in vitro. Methods： Hepat＇ocytes, hepatic stellate cells （HSCs）, hepatoma cell line （HepG2） and human peripheral blood mononuclear cells （PMNCs） were studied with respect to proliferation, extracellular matrix production and apoptotic activities by proliferation assay, radioimmunoassay, gene transfection, reporter gene analysis and flow cytometry, respectively. Results： A strong stimulatory proliferation effect was observed in hepatocytes, and an inhibitory effect was found in HSCs. Hyaluronic acid （HA） production and reporter gene activities driven by various etl（I） procollagen gene promoters in HSC-T6 were significantly decreased after treatment with the extract. Fluo-Anexin V binding apoptotic HepG2 cells were more prominent in the presence of 60ug/ml extract. More CD4^＋/CD69^＋ positive T lymphocytes existed in the presence of the extract. Conclusion： Porcine liver extract is effective for antifibrogenesis via hepatocyte regeneration, HSC and hepatoma cell inhibition in vitro. The elevation of active T lymphocytes is helpful for immune surveillance. Fine mapping of the extract is necessary in order to get definite molecules which are essential in all described functions.     

放射线对肝癌细胞中端粒酶启动子活性的影响

目的:研究放射线对不同的肝癌细胞株中人端粒酶逆转录酶(hTERT)启动子活性的影响。方法:报告质粒phTERTp-Luc经脂质体METAFECTENE介导瞬时转染4种不同的肝癌细胞株后,给予2 Gyγ射线照射,观察照射组与非照射组中荧光素酶的表达。结果:瞬时转染4种不同的肝癌细胞株后,检测到照射可经hTERT启动子而诱导荧光素酶的表达增强,即hTERT启动子在2 Gy照射组中比在非照射组中具有更高的活性。结论:2Gyγ射线可以诱导肝癌细胞中hTERT启动子活性增强,有望用于增强肝癌细胞中hTERT启动子介导的靶向基因治疗,并为进一步深入研究奠定了基础。     

血卟啉衍生物（HPD）加光照对无细胞体系DNA合成的抑制作用

本文从腹水型肝癌细胞部分纯化了依赖于ＤＮＡ的ＤＮＡ聚合酶ａ，以热变性的小牛胸腺ＤＮＡ为模板，观察了ＨＰＤ的光敏效应对这－ＤＮＡ合成体系的影响。结果表明，单独的ＨＰＤ或光照对无细胞体系ＤＮＡ合成无细胞；而既用ＨＰＤ处理，又经光照后的ＤＮＡ合成系统表现出明显的抑制作用。此抑制作用呈显著的剂量和时间的依赖效应关系。ＨＰＤ的光敏反应与ＤＮＡ模板非竞争性地作用于酶。增加模板量不能使抑制作用逆转；而增加酶量可     

血清前白蛋白检测在诊断肝硬化并发肝癌中的应用

检测了１０８例正常成人参考样本，３７例经手术和病理活检确诊的肝硬化并发肝癌患者，１２例与肝损害关系不大的良性疾病患者血清ＰＡ含量。实验表明，ＰＡ对肝硬化并发肝癌诊的阳性预告值（＋ＰＶ）为１００％，阴性预告值（－ＰＶ）８０．０％，诊断敏感度９１．８９％，特异性１００％，准确度９３．８８％；ＰＡ对该病的诊断敏感度明显优于ＡＬｂ，γ－ＧＴＩＣＧＲ１５，ＡＬＴ，ＡＬＰ等几项目前常用生化指标；并指出ＰＡ明显     

徐长卿水提物抗肝癌作用初探

目的 探讨徐长卿水提物抗肝癌的作用。方法 以徐长卿水提物作用于体外培养的HepG-2细胞株，观察其对HepG-2细胞生长曲线的影响及评价其抗肝癌作用。结果 徐长卿水提物浓度为60g／L和30g,／L时，能使HepG-2细胞的生长受到抑制，15g／L和7．5g／h时对细胞增殖没有明显的抑制作用。结论 徐长卿水提物浓度为60g／L和30g／L时，在体外细胞培养中对HepG-2细胞增殖有较好的抑制作用。     

Glycogen storage disease type I: pathophysiology of liver adenomas

Of the many complications associated with glycogen storage disease type I, hepatic tumours cause great concern because of their malignant potential and the current difficulties in monitoring them. Hepatic adenomas occur in 22%–75% of affected adults, according to the population studied, and from those reported in the literature are thought to have an approximately 10% risk of undergoing malignant transformation. Their aetiology is unclear, but they occur generally in postpubertal patients, and can be either single or multiple. This article discusses theories of their aetiology, methods of detection and monitoring, and treatment options.Conclusion: the incidence of liver tumours in younger adults seems less than in older ones, suggesting that better dietary treatment, and thus improved metabolic control, may be protective. Surgery (partial hepatectomy or orthotopic liver transplantation) is the definitive therapy for these tumours, but the timing of this intervention is difficult to determine and it is not without its own hazards. Published online: 12 July 2002     

EGF对肝癌细胞及正常肝细胞内ras基因表达的影响

目的 揭示正常肝细胞和肝癌细胞对表皮生长因子（ｅｐｉｄｅｒｍａｌ ｇｒｏｗｔｈ ｆａｃｔｏｒ,ＥＧＦ）不同的应答能力,以及ＥＧＦ对两种不同细胞株内ｒａｓ基因表达的影响。方法 运用Ｗｅｓｔｅｒｎ免疫印迹技术对正常肝细胞株Ｌ－０２和肝部细胞株Ｈｅｐ－Ｇ２ｋ ｐ２１＾ｒａｓ的表达进行比较分析。结果 在两种细胞株Ｌ－０２和Ｈｅｐ－Ｇ２中以ｐ２１＾ｒａｓ的表达。Ｈｅｐ－Ｇ２细胞的ｐ２１＾ｒａｓ表达量在ＥＧＦ     

盼生安对BEL-7402肝癌细胞的诱导分化作用

目的研究盼生安对BEL-7402肝癌细胞的诱导分化作用。方法应用细胞生物学的方法观察盼生安对细胞生长特性的影响；形态计量学方法研究对细胞、细胞核、核浆比及AgSORs形态计量学参数的影响；流式细胞术测定细胞周期；常规生化测定对ALP和LDH酶活力的影响；RNA点印记杂交测定对c-N-ras癌基因表达的影响。结果经盼生安处理后，可使BEL-7402肝癌细胞生长速度显著减慢。细胞及细胞核的面积、等效直径、等效体积、核浆比均随着剂量的增大而减小．同时AgNORs颗粒和面积明显减少。细胞周期出现向G0／G1期移行的特征动力学改变。两种肝癌标志酶ALP及LDH活性降低，且有较好的剂量．效应关系。c-N-ras癌基因表达受到抑制。结论初步表明盼生安具有诱导BEL-7402肝癌细胞分化的作用。