pH指示剂吸收度比值法测定盐酸乙胺丁醇的含量

本文报道pH指示剂吸收度比值法测定盐酸乙胺丁醇的含量。该法采用指示剂为0.05%茜素黄R,测定波长374nm和500nm;标准液为0.1mol/L NaOH;仪器为WFZ-800D_2型分光光度计。测得三批盐酸乙胺丁醇的含量分别为99.5±0.04%,99.4±0.05%和99.6±0.03%.同时与药典法比较,结果一致。     

肺结核健康相关生存质量测定量表的研制概况及策略

通过综合查阅国内外结核生存质量相关研究的文献,探讨国内外肺结核生存质量研究的概况,为进一步研制肺结核病健康相关生存质量测定量表提供依据。国内外有关结核病生存质量研究的报告主要集中在研究结核对患者生存质量的影响或采用SF-36等普适性量表进行测定,国内外均缺乏成熟的结核生存质量特异性测定量表。为配合现代结核病控制策略,帮助掌握肺结核病人详细的生存质量资料,需要按照国际上科学、规范和标准化的方法,开展考虑到生存质量测定量表等价性等问题的、便于比较和运用的结核病健康相关生存质量测定量表的研制。     

2,6—二叔丁基对甲酚对大鼠尿生化指标的影响

以２０，６０和１８０ｍｇ／ｋｇ体重剂量给大鼠喂食２，６－二叔丁基对甲酚（ＢＨＴ），检测尿中ＡＳＴ、ＡＬＰ、ＬＤＨ和ＧＧＴ等指标变化。结果表明：随染毒时间延长，各尿酶含量逐渐出现显著性增高，病理学检查发现高剂量组有１例间质性肾炎，提示ＢＨＴ对肾功能可能产生有害作用。     

低温蒸汽甲醛气体灭菌效果观察

为观察低温蒸汽甲醛气体灭菌效果及其使用的可行性,采用每包内放置化学指示卡和生物指示剂,进行了实际灭菌效果检测。结果,灭菌程序记录曲线清晰,可清楚地记录灭菌日期、抽真空次数、进甲醛次数、灭菌维持时间、灭菌温度、压力、抽甲醛气体次数、空气脉冲次数和结束时间。所有包装袋外甲醛化学指示色块均由原来的橙色变为棕色;所有包内化学指示卡均由原来的深兰色变为合格的绿色。26锅次灭菌后生物指示剂全部无菌生长;对 36件灭菌后物品进行无菌检验均无细菌生长。结论,低温蒸汽甲醛灭菌效果可靠,操作简便,使用安全。     

Physiological pharmacokinetics of solutes in the isolated perfused rat hindlimb: Characterization of the physiology with changing perfusate flow,protein content,and temperature using statistical moment analysis

Distribution of Evans Blue (EB), sucrose, and water into the isolated perfused rat hindlimb was studied under various conditions using the multiple indicator dilution (MID) technique. Statistical moment analyses of the outflow profiles for the EB, sucrose, and water were used to define the vascular, extravascular, and total water spaces, respectively. The varied perfusion conditions included albumin content (2, 4.7, and 7%), temperature (25, 37, and 42 C), perfusate flow rate (2, 4, 8, and 12 ml/min) and the presence/absence of red blood cells. The range of studies undertaken were chosen to represent the variety of conditions used in the preparation of both isolated animal and human limbs, the latter being particularly important in cytotoxic therapy for recurrent malignant melanoma. The distribution volumes of EB, sucrose, and water were dependent on the flow rate and the albumin content of perfusate. The normalized variances (CV ² ) of the markers were of the following order: sucrose (2.18) > water (1.58) > EB (0.68), indicating that some disequilibrium occurs during the capillary exchange of water and sucrose. It is suggested that a Krebs-Henseleit buffer containing 2% BSA is a suitable perfusate for most studies of the isolated rat hindlimb perfusion. The effect of albumin concentration manifests itself only at higher flows.We acknowledge the support of the National Heart Foundation (Queensland) and the Mayne Bequest Foundation. This study was conducted while the investigator (Z.Y. Wu) was in receipt of a WHO Research Training Grant. Professor M. S. Roberts also acknowledges the support of the Queensland and Northern New South Wales Lions Kidney & Medical Research Foundation.     

RELATIONSHIP BETWEEN PLATELET CYTOSOLIC FREE CALCIUM CONCENTRATION AND PLASMA RENIN ACTIVITY

1. Plasma renin activity (PRA) and platelet cytosolic free calcium concentration ([Ca2+]i) were simultaneously determined in 18 untreated essential hypertensive subjects and 17 normotensive controls. A significant positive correlation was found between [Ca2+]i and PRA (slope = 42 nmol/l/ng/ml/h) in these 35 subjects. 2. Two determinations more than one week apart in nine subjects confirmed the parallel fluctuations of [Ca2+]i and PRA. A strict sodium restriction produced a progressive PRA elevation associated with a parallel rise in [Ca2+]i in one subject. 3. These results are consistent with the hypothesis that angiotensin II causes a concentration-dependent calcium mobilization.     

Relationship of Spinal Fracture to Bone Density,Textural, and Anthropometric Parameters

To investigate risk factors for spinal fracture, we studied the relationship between the prevalence of asymptomatic spinal fracture and various morphological measures including spinal bone mineral density (BMD) in women. A total of 122 women ranging in age from 55 to 79 years were studied. The group consisted of 46 women aged 55–59 years (18 with fracture), 51 women aged 60–69 years (26 with fracture), and 26 women aged 70–79 years (14 with fracture). BMD of cortical and trabecular bone from L1 to L3 was measured using quantitative computed tomography (QCT). Run-length analysis was applied to evaluate the spinal trabecular textural features using CT images; the texture indices which represent the mean width of trabeculae (the T-texture) and that of intertrabecular spaces (the I-texture) were obtained. Anthropometric factors including body weight and height, psoas muscle area, and vertebral bone volume were measured using CT images. Among the various factors, trabecular BMD in women aged 55–69 years showed the highest odds ratio for the presence of fracture per standard deviation (SD) decrease in bone density. However, in women aged 70–79 years, the highest odds ratio was observed for trabecular texture index but not for trabecular BMD. The I-texture in women aged 55–59 years, the muscle area in women aged 60–69 years, and cortical BMD and muscle area in women aged 70–79 years were also considered significantly related to the risk of fracture. Received: 31 December 1995 / Accepted: 24 July 1996     

心理训练对高特质焦虑新兵生理指标和考核成绩的影响

目的研究心理训练对高特质焦虑新兵考核时生理指标和成绩的影响。方法采用个人评价问卷（PEI）、状态．特质焦虑问卷（STAI）和简易应对方式问卷对参加考核的新兵实施团体测试。将被试者分为对照组、低特质焦虑组、高特质焦虑组和干预组，对干预组进行自我评价、潜能开发、团队精神强化、应对方式的心理训练。测试考核前后生理指标差值及考核成绩的变化。结果干预组新兵军事考核前后收缩压、舒张压和脉搏三项生理差值与考核成绩明显优于高特质焦虑组（P〈0．05）。结论系统的心理训练可有效改善新兵对应激情景的生理和心理反应，提高作业绩效。     

Development of multielectrode impedance plethysmography

The feasibility of simultaneous independent measurements of impedance variations in the right and left apex and base of the lungs using the technique of multielectrode impedance plethysmography (MIPG) was investigated. To obtain independent impedance measurements in each region, high impedance sensitivity areas must be localised by weighting the impedance sensitivity distribution. 12 planar coaxial-type electrodes were attached on the right and left upper, middle and lower sites of the anterior and posterior chest walls. Currents of identical absolute values and differing polarities were simultaneously applied to neightbouring electrodes and voltage measurements were carried out sagittally at the right and left upper and lower sites of the chest walls. The effect of weighting the impedance sensitivity distribution was verified through experimental studies on mongrel dogs. The methods utilised for the induction of regional physiological conductivity changes were selective ventilation and selective indicator infusion into the pulmonary vasculature. The detected impedance variation showed reasonably indenpendent responses which were consistent with our expectations from the results of the computer simulation.