建立手术室临时医嘱本 规范护理行为

目的为规范手术室护士的护理行为,准确完整记录护士在手术中执行医嘱的具体活动,做好手术室安全护理工作.方法建立手术室临时医嘱本,护士根据医嘱用药.结果减少手术护理工作中的缺陷,增强手术室护士的法律意识,增强了巡回护士工作的主动性和计划性.结论在手术室建立临时医嘱本,有利于进一步规范手术室护理行为,预防和减少护理缺陷发生.     

图书馆全开放管理模式下汇文系统的图书清点

为摸清图书馆的"家底",掌握馆藏的实际情况和一段时间内财产丢失情况,发现并纠正业务工作中存在的一些问题,保证为读者提供准确可靠的数据,应用江文系统典藏模块的馆藏清点功能,利用数据采集器采集条码,清点图书37.86万册,其中丢失2063册,丢失率为0.54%.由此得出结论,开放式管理模式馆藏清点需做好清点前的充分准备,选择适当的清点方式,对清点过程中出现的问题做出记录并及时解决.     

图书附件的编目及管理探讨

阐述了图书附件的定义及不同形态,并依据普通图书型、表图型、计算机阅读型、声像型等附件形态,详细介绍了带附件图书的编目和管理方法。     

统一订购，分校区验收分编模式的探索与思考

介绍了高校图书馆图书采编工作新模式,即统一订购分校区验收分编图书的基本要求、基本做法,并分析了这一模式的优点和不足。     

妇科应用信息本和个人考核本的体会

目的设立信息本和个人考核本,对身边发生的每件事、护理人员存在的问题和优点进行随时记录,通过有意识导向与控制,提高护理质量。方法对身边发生的每一件事情、护理人员存在的问题和优点进行随时记录。结果设立信息本为护理人员提供信息,使她们能够随时阅读,加强了护士长与护士间信息交流的互动,采用个人考核本能够对护理人员存在的问题和优点进行详细记录,通过有意识导向与控制,提高护理质量。结论设立信息本使护士长与护士间的沟通变得更加快捷、直接和有效,个人考核本的设立,能够对护士的问题和优点进行详细记录,使她们及时发现自己的长处和短处,并在此基础上取长补短,从而更加出色地完成本职工作,提高了护理质量。     

Experimental evidence for a link among cupredoxins: red, blue, and purple copper transformations in nitrous oxide reductase

The cupredoxin fold is an important motif in numerous proteins that are central to several critical cellular processes ranging from aerobic and anaerobic respiration to catalysis and iron homeostasis. Three types of copper sites have been found to date within cupredoxin folds: blue type 1 (T1) copper, red type 2 (T2) copper, and purple Cu(A). Although as much as 90% sequence difference has been observed among some members of this superfamily of proteins that span several kingdoms, sequence alignment and phylogenic trees strongly suggest an evolutionary link and common ancestry. However, experimental evidence for such a link has been lacking. We report herein the observation of pH-dependent transformation between blue T1 copper, red T2 copper, and the native purple Cu(A) centers of nitrous oxide reductase (N2OR) from Paracoccus denitrificans. The blue and red copper centers form initially before they are transformed into purple Cu(A) center. This transformation process is pH-dependent, with lower pH resulting in fewer trapped T1 and T2 coppers and faster transition to purple Cu(A). These observations suggest that the purple Cu(A) site contains the essential elements of T1 and T2 copper centers and that the Cu(A) center is preferentially formed at low pH. Therefore, this work provides an underlying link between the various cupredoxin copper sites and possible experimental evidence in vitro for the evolutionary relationship between the cupredoxin proteins. The findings also lend physiological relevance to cupredoxin site biosynthesis.     

网络时代医学书评的资源建设与发布推广

医学书评关注的是医学图书中学术思想及理论知识界的最新动态,推荐最新学术成果,所评医学图书必须具有学术传承价值并能直面医学发展中遇到的新问题及新挑战。对于医学院校图书馆的资源建设来说,书评可以说是医学知识积累与发展的助推器。本文梳理了中国医科院图书馆近20年医学专家书评工作,介绍了中国医科院图书馆医学书评资源建设的经验,还探索了书评网络平台发布与推广的方式。     

Mitochondria and chloroplasts as the original sites of melatonin synthesis: a hypothesis related to melatonin's primary function and evolution in eukaryotes

Mitochondria and chloroplasts are major sources of free radical generation in living organisms. Because of this, these organelles require strong protection from free radicals and associated oxidative stress. Melatonin is a potent free radical scavenger and antioxidant. It meets the criteria as a mitochondrial and chloroplast antioxidant. Evidence has emerged to show that both mitochondria and chloroplasts may have the capacity to synthesize and metabolize melatonin. The activity of arylalkylamine N‐acetyltransferase (AANAT), the reported rate‐limiting enzyme in melatonin synthesis, has been identified in mitochondria, and high levels of melatonin have also been found in this organelle. From an evolutionary point of view, the precursor of mitochondria probably is the purple nonsulfur bacterium, particularly, Rhodospirillum rubrum, and chloroplasts are probably the descendents of cyanobacteria. These bacterial species were endosymbionts of host proto‐eukaryotes and gradually transformed into cellular organelles, that is, mitochondria and chloroplasts, respectively, thereby giving rise to eukaryotic cells. Of special importance, both purple nonsulfur bacteria (R. rubrum) and cyanobacteria synthesize melatonin. The enzyme activities required for melatonin synthesis have also been detected in these primitive species. It is our hypothesis that mitochondria and chloroplasts are the original sites of melatonin synthesis in the early stage of endosymbiotic organisms; this synthetic capacity was carried into host eukaryotes by the above‐mentioned bacteria. Moreover, their melatonin biosynthetic capacities have been preserved during evolution. In most, if not in all cells, mitochondria and chloroplasts may continue to be the primary sites of melatonin generation. Melatonin production in other cellular compartments may have derived from mitochondria and chloroplasts. On the basis of this hypothesis, it is also possible to explain why plants typically have higher melatonin levels than do animals. In plants, both chloroplasts and mitochondria likely synthesize melatonin, while animal cells contain only mitochondria. The high levels of melatonin produced by mitochondria and chloroplasts are used to protect these important cellular organelles against oxidative stress and preserve their physiological functions. The superior beneficial effects of melatonin in both mitochondria and chloroplasts have been frequently reported.