高效液相色谱法测定红景天中酪萨维含量

目的建立红景天中酪萨维含量测定方法。方法采用高效液相色谱（HPLc）法。色谱柱为KromasilC19柱（150mm×4．6mm,5μm）,流动相为甲醇-7K（50：50）,检测波长为270nm,柱温为室温,流速为1．0mL／min。结果酪萨维进样量在5．2～52Ixg范围内与峰面积线性关系良好（r=0．9998）,平均加样回收率为98．60％,RSD=1．54％（n=6）。结论所建立的HPLC法操作简便、快速,灵敏度高,结果准确可靠,专属性强,重现性好,可用于红景天药材的质量控制。     

复方甘草酸单铵治疗玫瑰糠疹疗效观察

目的探讨复方甘草酸单铵治疗玫瑰糠疹的疗效及其安全性。方法70例玫瑰糠疹患者分为2组,治疗组40例,给予复方甘草酸单铵注射液40ml,ivgtt,qd,;对照组30例,给予左西替力嗪5mg,qd,两组均外用炉甘石洗剂,每天2次;10d为一疗程。结果治疗组痊愈率及总有效率分别为72．5％和90．00％,对照组分别为53．33％和66．67％,两组比较均有显著性差异（P〈0．01）,两组均未见明显不良反应。结论复方甘草酸单铵治疗玫瑰糠疹疗效确切,疗程短,不良反应少,值得临床应用。     

抗组胺药联合物理疗法治疗玫瑰糠疹疗效观察

目的探讨抗组胺药联合窄谱中波紫外线（NB-UVB）治疗玫瑰糠疹（PR）的疗效。方法将96例诊断明确的PR患者,随机分治疗组（50例）和对照组（46例）,两组患者性别、年龄、病情之间无明显差别,具有可比性。治疗组在抗组胺药物治疗同时接受NB-UVB治疗仪治疗,对照组仅予抗组胺药物治疗。治疗3周后同时评价疗效。结果治疗组平均治愈时间（9.21d）明显短于对照组（21.32d）,P〈0.01。3周后对照组痊愈20例,显效10例;治疗组痊愈47例,显效2例。治疗组治愈率显著高于对照组（P〈0.01）。结论抗组胺药联合NB-UVB治疗PR疗效好、安全、方便,值得推广应用。     

Malassezia species isolated from lesional and non-lesional skin in patients with pityriasis versicolor

Pityriasis versicolor (PV) is a superficial fungal infection where Malassezia species play a definite causative role, but the clinical significance of each of these species is not fully understood. The aim of our study was to analyse the prevalence of Malassezia species in PV lesions and to examine if the range of species varies with patient sex, age, direct microscopy findings and some clinical data. Ninety patients with PV completed the study. The samples were obtained by scraping the skin surface, both from lesional and non-lesional skin and then incubated on Sabouraud dextrose agar and modified Dixon agar. The yeast isolated were identified according to their macroscopic and microscoipic features and physiological characteristics. In PV lesions, the most common species was M. globosa (63%), followed by M. sympodialis (14%), M. furfur (10%), M. obtusa (8%) and M. slooffiae (4%). The most frequently isolated species from clinically healthy skin were M. globosa (49%), M. sympodialis (37%) and M. furfur (5%). We found significant difference in the distribution of Malassezia species between lesional and non-lesional skin and in the distribution of Malassezia species according to the direct microscopy findings. M. globosa in its mycelial phase is the predominant species involved in the aetiology of PV.     

Pityriasis alba: a study of pathogenic factors

BACKGROUND: The aetiology of pityriasis alba (PA), a common dermatosis in childhood, is still controversial. The objective of this study was to assess the possible aetiopathogenic factors of this disease in infants. METHODS: Forty-four patients with PA and 31 healthy children were examined and compared. Personal hygiene habits, sun exposure, presence of Staphylococcus aureus in nasal fossae and presence of major or minor signs of atopy were assessed during anamnesis and physical examination. Susceptibility to ultraviolet (UV) B radiation was measured by the onset of a contact hypersensitivity reaction to diphenylcyclopropenone in individuals sensitized in previously irradiated areas. RESULTS: The prevalence of PA was higher in individuals with darker skin, in high phototype categories, as well as in males. The number of daily baths and sun exposure between 10.00 h and 15.00 h were significantly higher in the PA group when compared with controls (P = 0.03 and P = 0.0015, respectively). The presence of atopy signs was more common in pityriasis patients (P = 0.002). Susceptibility to UVB radiation was 29.6% in the PA group vs. 29.0% in the control group; nevertheless, important differences were found after stratification in order to control possible confounding factors. The presence of S. aureus in the nostrils was equal in both groups. CONCLUSIONS: Our results confirm that PA, in our population, is more prevalent in males and in individuals in higher phototype categories. In those with inadequate personal hygiene and sun exposure habits the disease is more accentuated, demonstrating that the xerosis presenting in individuals with atopic diathesis is an important element in the development of the disease. S. aureus is not an important aetiopathogenic factor in PA. Susceptibility to UVB becomes important when related to the patient's phototype.     

冀产红景天抗应激作用的实验研究

本文药理实验研究表明,冀产三种红景天(红景天Rhodiola rosea;狭叶红景天R.kirilowii;小丛红景天R.dumulosa)煎剂具有显著或非常显著地抗疲劳、耐缺氧、耐寒、耐高温等抗应激作用。     

马拉色菌对角质形成细胞分泌黑素合成相关细胞因子的影响

目的探讨引起花斑癣不同临床色素表现的马拉色菌与角质形成细胞共培养,导致与黑素合成相关的细胞因子的变化。方法MTT法筛选不同比例的马拉色菌对角质形成细胞增殖率的影响;用色沉和色减区分离的马拉色菌与角质形成细胞共培养,在不同时间段收集上清液,ELISA法测定碱性成纤维细胞因子（b-FGF）、内皮素-1（ET-1）、神经生长因子-β（NGF-β）、白介素-1α（IL-1α）、白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和干细胞因子（SCF）的动态变化。结果角质形成细胞与马拉色菌在1∶10比例以下,角质形成细胞的生长状况不受马拉色菌的影响（P〉0.05）。当比例提高至1∶20以上时,角质形成细胞的生长受到显著抑制（P〈0.01）。马拉色菌刺激角质形成细胞分泌IL-1α、IL-6、TNF-α、ET-1增加（P〈0.01）。未检测到b-FGF、NGF-β和SCF的产生（P〉0.05）。色沉区马拉色菌刺激产生的ET-1显著高于色减区（P〈0.01）。结论马拉色菌刺激角质形成细胞分泌黑素合成相关因子的能力不同。ET-1在花斑癣色素沉着中可能起了一定作用。     

The spectrum of Malassezia species isolated from students with pityriasis vesicolor in Nigeria

Pityriasis versicolor (PV) is a common superficial fungal infection of the skin caused by Malassezia. Initially M. furfur was suggested as its main aetiological agent; however, more recent studies suggest M. globosa as the dominant species. The possibility of a variance in predominant species based on geographical basis has not been fully evaluated. The objective of this study was to identify the Malassezia species on affected and non‐affected skin of students with PV who reside in a tropical environment (Abuja, Nigeria) and correlate them to clinical characteristics. In this study, the literature on prevalence of Malassezia genus in PV was also reviewed. Samples were taken from 304 PV lesions and 110 normal appearing skin. Microscopy, culture and identification of Malassezia species utilising polymerase chain reaction–restriction fragment length polymorphism analysis were performed. Three Malassezia species were detected in PV with the major species being M. furfur. On normal appearing skin, M. furfur (77.6%) and M. restricta (10.4%) were both detected. No case of M. globosa was identified in this study. There was no significant difference between species identified and clinical features of PV. M. furfur is probably still the most predominant species causing PV in the tropical environment.     

高效液相色谱法测定维药蜀葵花中芦丁和紫云英苷含量

目的：建立高效液相色谱法（HPLC 法）测定维药蜀葵花中芦丁和紫云英苷的含量。方法采用YMC-Pack ODS-A C18（4．6 mm＆#215;250 mm,5μm）色谱柱,以流动相 A（0．2％磷酸水溶液）和流动相 B（0．2％磷酸乙腈溶液）进行梯度洗脱,检测波长为360 nm,流速为0．6 mL/min,进样体积10μL。结果芦丁和紫云英苷分别在0．5～6．0μg/mL 和1．0～12．0μg/mL 浓度范围内呈良好线性关系;线性方程分别为 A ＝583．5C -0．623和 A ＝628．3C -0．27,相关系数 r 分别为0．9995和0．9991;芦丁和紫云英苷的平均加样回收率（n ＝6）分别为102．3％和100．8％,RSD 分别为0．89％和0．60％,蜀葵花样品中芦丁和紫云英苷的含量分别为0．30～0．32 mg/g 和0．43～0．46 mg/g。结论本方法简便、准确,重复性好,可用于维药蜀葵花中芦丁和紫云英苷的含量测定,为其质量控制提供参考。