高效液相色谱法测定维药蜀葵花中芦丁和紫云英苷含量

目的：建立高效液相色谱法（HPLC 法）测定维药蜀葵花中芦丁和紫云英苷的含量。方法采用YMC-Pack ODS-A C18（4．6 mm＆#215;250 mm，5μm）色谱柱，以流动相 A（0．2％磷酸水溶液）和流动相 B（0．2％磷酸乙腈溶液）进行梯度洗脱，检测波长为360 nm，流速为0．6 mL/min，进样体积10μL。结果芦丁和紫云英苷分别在0．5～6．0μg/mL 和1．0～12．0μg/mL 浓度范围内呈良好线性关系；线性方程分别为 A ＝583．5C -0．623和 A ＝628．3C -0．27，相关系数 r 分别为0．9995和0．9991；芦丁和紫云英苷的平均加样回收率（n ＝6）分别为102．3％和100．8％，RSD 分别为0．89％和0．60％，蜀葵花样品中芦丁和紫云英苷的含量分别为0．30～0．32 mg/g 和0．43～0．46 mg/g。结论本方法简便、准确，重复性好，可用于维药蜀葵花中芦丁和紫云英苷的含量测定，为其质量控制提供参考。

Efficient determination of rutin and astragalin in Althaea rosea（L .）Gavan from Uyghur medicine

Objective HPLC method was used to determinate the content of rutin and astragalin inAlthaea rosea （L .）Gavan from Uyghur Medicine.Methods Analysis was carried out on an YMC-Pack ODS-A C18 （4.6 mm＆#215;250 mm,5 μm）column with 0.2% phosphoric acid in water 0.2% phosphoric acid in acetonitrile as mobile phase in gradient elution.The detective wavelength was 360 nm,flow rate was 0.6 mL/min and injec-tion volume was 10 μL.Results The linear ranges of rutin and astragalin were 0.5-6.0 μg/mL and 1.0-12.0 μg/mL and the linear equation were A =583.5C -0.623 （r =0.999 5）and A =628.3C - 0.270 （r =0.999 1）respectively.The average recoveries were 102.3% and 100.8% with RSD of 0.89% and 0.60% （n=6）respectively.The content of rutin and astragalin in Althaea rosea （L .）Gavan were 0.30-0.32 mg/g （n =3）and 0.43~0.46 mg/g （n =3）respectively.Conclusion The results show that the method is sim-ple,accurate and repeatable,and can be used for the quantitative analysis of rutin and astragalin inAlthaea rosea （L.）Gavan from Uyghur Medicine and can be used as a referent for its quality control.