巴戟天多糖对精索静脉曲张大鼠睾丸修复作用的蛋白质组学分析*

目的：从蛋白质组学层面探讨精索静脉曲张（VC）的病理机制及巴戟天多糖（MOP）对VC大鼠睾丸修复 作用的机制。方法：健康雄性SD大鼠随机分为假手术组、VC模型组、VC+MOP给药组。采用串联质谱标签法 对大鼠左侧睾丸组织进行蛋白质组学分析，筛选差异表达蛋白（ 差异倍数>25%），并进行生物信息学分析。结 果：VC模型组和假手术组相比差异蛋白总数为46 个，VC+MOP给药组与VC模型组相比差异蛋白总数为21 个， VC+MOP给药组与假手术相比差异蛋白总数为5 个。差异表达蛋白层次聚类分析结果显示，VC+MOP给药组与 假手术组之间蛋白表达相似，而VC模型组与其他2 个组差异较大。基因本体（GO）和日本京都基因和基因组百 科全书（KEGG）通路富集分析显示，差异蛋白主要富集于核糖体生物合成、细胞凋亡信号、激素水平调节、血 液循环调节、体液免疫应答、蛋白激酶C信号转导、肽激素分泌通路。结论：MOP可使由VC引起的组间睾丸差 异表达蛋白数量减少，逆转由VC引起的蛋白表达水平异常，提示VC的病理机制和MOP的修复作用可能与关键 蛋白的差异表达以及富集的GO和KEGG通路有关。     

肤痒颗粒联合盐酸氯环利嗪治疗慢性荨麻疹临床疗效研究

目的观察肤痒颗粒联合盐酸氯环利嗪治疗慢性荨麻疹的临床疗效和安全性。方法治疗组42例口服肤痒颗粒,4.5 g/次,3次/d,盐酸氯环利嗪片25 mg,2次/d;对照组42例仅服盐酸氯环利嗪片,用法和剂量同治疗组。治疗4周判定疗效。随访4周观察复发或加重情况。结果治疗组有效例数35例,有效率83.33%;对照组有效例数26例,有效率61.90%。两组比较χ2=4.85,P0.05。随访治疗组复发9例,加重16例,复发加重率59.52%;对照组复发7例,加重27例,复发加重率80.95%。两组复发加重率比较,χ2=4.61,P0.05。两组均有近半数病例有轻度嗜睡、头晕、口干等症状。结论肤痒颗粒联合盐酸氯环利嗪治疗慢性荨麻疹安全,疗效好。     

The sorting mechanism underlying the separation of salivary proteins into secretory granules in parotid glands

BackgroundBecause of their ability to synthesize and secrete large amounts of proteins, salivary glands are considered target organs for gene therapy. Acinar cells of salivary glands are polarized and secrete proteins via two routes, apical and basolateral pathways, i.e., saliva and blood. It is necessary to deliver exogenous proteins as drugs via appropriate routes for efficient supply. We analyzed the sorting mechanism using an expression system of a reporter protein, HaloTag, to control secretory pathways in salivary glands.HighlightWe found that the HaloTag protein that has only the signal peptide sequence of amylase (SS25H) was transported to secretory granules and secreted upon β-adrenergic stimulation. However, the basal secretion without stimulation was slightly higher than that of amylase, suggesting that SS25H was partially transported via a constitutive secretory pathway. Because the signal peptide sequence is deleted after translocation into the endoplasmic reticulum, SS25H contains only the HaloTag amino acid sequence and no specific sorting signal when sorted in the trans-Golgi network. In addition, SS25H did not form oligomers in secretory granules.ConclusionThese results suggest that the sorting of SS25H is completely passive. Thus, it can be used as standard protein that is not subject to any regulation for sorting. It will be useful to study the effect of fusion with other proteins on transport efficiency via regulated secretory pathways. Using the HaloTag technology provides useful information about granule biogenesis and sorting mechanisms.     

应激颗粒的形成和功能特点及其与疾病的相关性研究进展

当真核细胞受到环境的刺激时,胞质中形成的致密颗粒物质,即为应激颗粒(SGs)。它具有抗氧化及抑制细胞凋亡的作用。mRNA结合蛋白的交互作用能促进应激颗粒的形成。转录后修饰和依赖ATP形成的RNP或蛋白重塑复合物起到调控应激颗粒的合成和分解的作用。应激颗粒具有流动性,而这种动态需要能量输入来维持。应激颗粒的形成能调节应激反应、病毒感染及信号传导过程。持续的和异常的应激颗粒的形成导致了神经退行性疾病和某些癌症的发生。     

正交试验法优选青紫消斑颗粒的醇提工艺

目的 优选青紫消斑颗粒中青黛、紫草、丹参的醇提工艺.方法 以β,β-二甲基丙烯酰胺阿卡宁转移率为考察指标,采用L9(34)正交试验优选紫草等醇提工艺.采用高效液相色谱法(high performance liquid chromatography,HPLC)测定阿卡宁含量,流动相乙腈:水:甲酸(70∶30∶0.05),检测波长:275 nm.结果 最佳提取工艺为95％乙醇,40℃温浸2次,第1次加10倍量,第2次加8倍量,每次24 h.结论 该工艺稳定可行,适用于青紫消斑颗粒的工业化生产.     

紫癜颗粒剂治疗过敏性紫癜对照观察

[目的]观察紫癜颗粒剂治疗过敏性紫癜疗效。[方法]将76例患者按随机数字表法,分为治疗组和对照组,每组38例。对照组采用西医常规方法进行治疗;治疗组口服紫癜颗粒剂(莲草、紫草、白茅根、茜草根、板蓝根、水牛角、仙鹤草、大黄、山豆根、连翘)。连续治疗4周判定疗效。[结果]治疗组总有效率94.70%,对照组总有效率73.70%,治疗组临床疗效优于对照组(P<0.05)。[结论]紫癜颗粒剂治疗过敏性紫癜疗效满意。     

Perforin and granzyme B involvement in oral lesions of lichen planus and chronic GVHD

J Oral Pathol Med (2010) 39 : 741–746 Background: Oral lesions of lichen planus and chronic graft‐vs.‐host disease (cGVHD) have similar clinical and histological features, but distinct etiology. Apoptosis induced by cytotoxic T lymphocyte has been proposed as a mechanism of keratinocytes death. Cytotoxicity can be mediated by granules containing granzyme B and perforin. Since common features can reflect similarities in immunological mechanisms, we studied the role of those molecules in both diseases. Methods: We analyzed 29 cases of oral lichen planus and 27 of oral cGVHD. The sections were studied on H&E, perforin and granzyme B staining. Results: The total means (epithelium plus connective tissue number) of the granzyme B‐ and perforin‐positive cells were significantly higher in cGVHD than in oral lichen planus lesions (P < 0.05). Also, it was found that the higher the number of perforin+ cells, the higher the number of granzyme‐B+ cells in the epithelium and in the connective tissue for both groups (P < 0.05). In oral lichen planus, the number of single apoptotic bodies had a positive correlation with connective tissue granzyme immunostaining and a negative correlation with perforin (P < 0.01). On the contrary, in oral cGVHD, the number of apoptotic body clusters presented a positive correlation with connective tissue perforin (P < 0.01). Conclusions: Our findings indicate that apoptosis in oral lichen planus seems to be correlated with granzyme B release, while in oral cGVHD, perforin seems to be more important. Although these diseases present clinical and histological similarities, subtle differences seem to exist in their pathogenetic mechanisms.     

风热感冒颗粒荆芥穗薄层色谱鉴别方法研究

目的：建立风热感冒颗粒的荆芥穗薄层色谱（TLC）鉴别方法。方法：对荆芥穗进行薄层色谱鉴别。结果：薄层色谱斑点清晰,阴性对照无干扰。结论：所用方法简便,重现性好,可用于风热感冒颗粒的质量控制。