Alternative efficient tritium labeling of repaglinide

There is a need for an efficient tritium labeling methodology for repaglinide to support numerous in vitro and in vivo studies. Numerous homogeneous and heterogeneous catalysts were screened for their capacity to tritiate repaglinide directly without the need to synthesize suitable precursors and to avoid multistep synthesis. In particular, boosting effects using a combination of homogeneous and heterogeneous catalysts were examined. Finally, a suitable method was selected for tritation and resulted in an efficient tritation of repaglinide via a heterogeneous catalyzed hydrogen–tritium exchange reaction with iridium black as the preferred catalyst.     

骨髓间充质干细胞同种移植治疗急性心肌梗死的实验研究

目的探讨骨髓间充质干细胞(MSCs)同种移植治疗急性心肌梗死的可行性。方法50只Wistar大鼠随机分为两组,对照组和移植组各25只。应用液氮冷冻法制作心肌梗死模型。移植组梗死部位注入经5-氮胞苷(5-aza)诱导和溴氮胞苷标记的大鼠MSCs,对照组梗死部位注入等容量DMEM液。分别于术前1天、术后1周和术后4周行超声心动图检查,以收缩期峰值速度(Vs)、左室舒张末容积(LVEDV)和左室射血分数(LVEF)为指标评价心功能。移植组于术后4周处死,移植心肌行溴氮胞苷免疫组化染色。结果对照组(n=13)和移植组(n=15)相比,术前及术后1周的心功能差异均无统计学意义(P>0.05),术后4周差异有统计学意义(P<0.05),术后4周移植组心功能明显优于对照组。移植部位免疫组化可见溴氮胞苷染色阳性细胞。结论经5-aza诱导的MSCs同种移植入大鼠急性心肌梗死模型的受损心肌后能存活并改善宿主的心功能。     

Existe-t-il un développement moteur et psychoaffectif spécifique de l’enfant à haut potentiel ? Influence sur les activités de la vie quotidienne et sur le profil du quotient intellectuel

ObjectiveThis study aimed to analyze the neurodevelopmental trajectory of motor milestones and psychoaffective development in children with high intellectual potential (IQ ≥ 130) regarding their IQ-profile (homogeneous/heterogeneous).Population and methodThe Wechsler Intelligence Scale (WISC-V), child's health record, and anamnestic questionnaire completed by parents were administered and analyzed in 76 healthy children (58 HIP/18 neurotypical), aged 6 to 13 (mean 10 years; SD 2.2). Seventy-six children with high intellectual potential were involved in the intragroup analysis (36 heterogeneous vs. 22 homogeneous). Two groups were matched for intergroup analyses: 18 neurotypical children (90 ≤ IQ ≤ 110) and 18 children with high intellectual potential (IQ ≥ 130).ResultsSignificant negative correlations were demonstrated between IQ and sitting (7 months) (r = ?0.32, P = 0.01, [95% CI = ?0.52 to ?0.09]), walking (12 months) (r = ?0.30, P = 0.03, [95% CI = ?0.51 to ?0.01], and expression of the first sentences (18 months) (r = ?0.60, P ≤ 0.001, [95% CI = ?0.71 to ?0.39]); with a significantly high rate of early development in children with high intellectual potential compared to neurotypical children. Significant correlations were also determined between VSI, WMI, and PSI with clumsiness in daily life abilities, games and school abilities, explaining 41% of the variance of heterogeneous IQ-profile of high intellectual potential. No significant difference was noted between children with high intellectual potential and neurotypical in psychoaffective characteristics. However, we demonstrated significant correlations between the heterogeneous IQ-profile of the HIP group and “isolation”, “separation anxiety”, “strong reaction to frustration” and “need for parents to be present during homework”.ConclusionEarly motor and language development has been demonstrated in children with high intellectual potential compared to neurotypical children regardless of the psychometric profile. Nevertheless, a significant part of the heterogeneous profile meets the DSM-5 criteria of a developmental coordination disorder (DCD), detected early with significant difficulties impacting daily life abilities, games and school abilities. The finding underlines the importance of analyzing the variability of index scores in the IQ-profile at school age in light of daily life abilities and anamnestic data.     

区域性同类检验检查项目警示信息系统的实现与应用

以区域性医疗信息平台为基础,结合医院端及中心端数据资源,在区域内对检验检查医嘱进行项目安全和重复同类警示,在提高医院医疗服务质量的同时,合理减少病人检验检查费用,实现医院信息从传统的院内信息到区域性医疗信息系统的转变.     

Asymptotics for threshold regression under general conditions

The inference of the threshold point in threshold models critically depends on the assumption that the density of the threshold variable at the true threshold point is continuous and bounded away from zero and infinity. However, violation of this assumption may arise in several econometric contexts such as treatment effects evaluation. This paper presents a thorough characterisation of the asymptotic distributions in the least‐squares estimation of such abnormal cases. First, the asymptotic results on the threshold point are different from the conventional case. For example, any convergence rate between zero and infinity is possible; the asymptotic distribution can be discrete, continuous or a mixture of discrete and continuous; the weak limits of the localised objective functions can be non‐homogeneous instead of homogeneous compound Poisson processes. Second, this paper distinguishes threshold regression from structural change models by studying a problem unique in threshold regression. Third, the asymptotic distributions of regular parameters are not affected by estimation of the threshold point irrespective of the density of the threshold variable. Numerical calculations and simulation results confirm the theoretical analysis, and the density of the threshold variable in an application is checked to illustrate the relevance of the study in this paper.     

均匀设计考察壳聚糖对杜仲叶提取液的絮凝澄清作用

目的利用均匀设计法探讨壳聚糖吸附澄清技术能否代替水沉淀法用于杜仲叶提取液的纯化工艺。方法用差示分光光度法对壳聚糖澄清法及水沉法沉淀后的药液所含的绿原酸含量进行测定。结果壳聚糖吸附澄清法与水沉法均能使药液澄清,前者更能保留药液中的有效成分。结论壳聚糖澄清法可替代水沉法用于精制杜仲叶提取液。     

同质TCP流的多重分形特性分析

采用基于小波变换模极大值的多重分形分析方法,讨论了大、小时间尺度下的结构函数和多重分形谱的特点。依据实例研究了不同时间下的同质TCP流(W eb)分别在大、小时间尺度下的分形特点;通过对不同协议的同质TCP流的协议本质及拥塞机制的分析,推断其所表现的多重分形谱特点与百分比的相关性;对同一TCP流中不同协议组成的分析,得到TCP总量与分量的多重分形谱特点以及与同质程度的关系。     

Evaluation of a nonhomogeneous endometrial echo pattern in the midluteal phase as a potential factor associated with unexplained infertility

OBJECTIVE: To determine whether the failure to develop a homogeneous hyperechogenic pattern in the midluteal phase is associated with decreased fecundity in infertile women who are not receiving follicle-maturing drugs. DESIGN: Observational study. SETTING: Outpatient infertility clinic of a University Medical Center. PATIENT(S): Two hundred ninety-six infertile women (> or =6 months) with regular menses, normal fallopian tubes and uterine cavity, and absence of severe male factor on their initial investigation cycle for follicular dynamic studies. INTERVENTION(S): Midluteal phase sonographic endometrial evaluation. MAIN OUTCOME MEASURE(S): Viable pregnancy rates (live fetus at end of first trimester) according to endometrial echo pattern in the midluteal phase. Other variables considered were age of patient, endometrial thickness and serum E(2) levels at midcycle and midluteal phase, midcycle echo pattern, and P levels in the midluteal phase. RESULT(S): The viable pregnancy rate was significantly higher in those women who exhibited a homogeneous hyperechogenic pattern (8.5%) compared to those women whose endometrium was found to be nonhomogenous (2.2%). No other confounding variables were found that could explain this outcome. CONCLUSION(S): A nonhomogeneous hyperechogenic sonographic endometrial echo pattern predicts lower fertility potential in women who are not receiving follicle-maturing drugs.     

Simple high-density lipoprotein cholesterol assay based on dry chemistry

Background: Currently, high-density lipoprotein cholesterol (HDL-C), a factor which prevents progression of arteriosclerosis, is measured using laboratory-based chemistry analyzers without a pretreatment step. Because HDL-C is measured with a pretreatment step in many point-of-care testing systems, a direct assay is needed. Methods: A dry-chemistry-based assay using surfactants has recently been developed in parallel with the development of a dedicated reagent. A simple analyzer that accepts whole blood samples was also developed. Results: The assay demonstrated excellent precision, dilution linearity and intermethod comparison. In an interference test, assay values tended to be lower in the presence of high concentrations of hemoglobin, conjugated or unconjugated bilirubin. Neither ascorbic acid up to 20 mg/dl, nor formazin turbidity up to 2100, had an effect on the assay. Conclusions: This dry-chemistry assay using only surfactants for specificity in the direct HDL-C method was judged useful for point-of-care instrumentation in terms of equipment compactness, operational simplicity and rapid responsiveness.     

用均匀设计法优选侧柏叶中槲皮苷的提取纯化工艺

用均匀设计法优选了测柏叶中槲皮苷的提取纯化工艺。结果表明：乙醇浓度、用量、浸泡时间以及水的用量、萃取溶剂体积对槲皮苷得率都有直接的影响；而氯化钠及pH值对得率影响不大。