Human chorionic gonadotropin and intravaginal natural progesterone are equally effective for luteal phase support in IVF

This prospective randomized study compared human chorionic gonadotropin (hCG) and micronized transvaginal progesterone for luteal support in 310 in vitro fertilization (IVF) patients treated with leuprolide acetate and gonadotropins in a long protocol, and showing normal ovarian response. Both treatment groups were homogeneous for age, BMI, stimulation treatment and ovarian response. Pregnancy rates per embryo transfer were not significantly different (33.1% for the hCG group versus 38.7% for the progesterone group). For IVF patients with a normal response to stimulation under pituitary supression, the use of hCG or progesterone for luteal support does not seem to have any effect on pregnancy rate. The choice of luteal treatment must balance medical hazard and patient convenience, as both therapeutic regimens seem equally effective.     

单核细胞被诱导表达淋巴管内皮标志物

目的淋巴管生成存在于肿瘤、炎症等许多疾病的病理生理过程中。本研究旨在探讨单核-巨噬细胞在炎症环境中转分化成淋巴管内皮细胞的可能性。方法取成人新鲜外周血,分离单核细胞,用ECM培养,分别在FN铺板或VEGF C、TNF α、LPS刺激下诱导培养24?h,用RT PCR和免疫荧光细胞化学方法检测单核-巨噬细胞对淋巴管内皮特异性标志物LYVE 1、Podoplanin、Prox1及内皮细胞共同标志物vWF、eNOS的基因和蛋白表达。结果在未诱导组,单核-巨噬细胞LYVE 1呈阳性表达,Podoplanin、Prox1、vWF、eNOS表达呈阴性;在FN铺板或VEGF C、TNF α、LPS诱导组,单核-巨噬细胞Podoplanin、Prox 1表达阳性,vWF和eNOS表达仍呈阴性。结论FN、VEGF C、TNF α、LPS均能有效刺激单核-巨噬细胞表达淋巴管内皮标志物。     

赵瑞华教授中药辅助子宫内膜异位症体外受精-胚胎移植经验

体外受精-胚胎移植(IVF-ET)是治疗子宫内膜异位症不孕的主要手段之一。赵瑞华教授对于中药辅助子宫内膜异位症不孕患者IVF-ET有着丰富的临床经验,采用因病制宜、病证结合分期疗法,改善卵巢功能和子宫内膜容受性,提高IVF-ET的成功率。降调节期以辨证论治为主,常用活血消异方加减;超促排卵及取卵期补肾健脾,调畅气机兼以活血;黄体期健脾补肾,调和营卫;孕期注重保胎,以补肾健脾,固冲安胎为法。     

牛磺酸抗肾上腺素诱发Langendorff家兔心脏心律失常的作用

本实验采用Langendorff家兔心脏观察了牛磺酸抗肾上腺素诱发心律失常的作用.实验发现10~(-5)～10~(-4)mol/L的牛磺酸不仅能减少肾上腺素诱发心律失常发生率,而且能延长诱发心律失常潜伏期和缩短心律失常持续时间的作用.     

Differentiation of osteoid-producing cellsin vitro: Possible evidence for the requirement of a microenvironment

Summary Periostea were dissected from 17-day-old chicken embryo calvariae, placed on millipore filters, and cultured on fluid media containing serum or on serum and plasma containing “plasma clots,” in three ways: 1) with the osteogenic layer facing the filter, 2) with the osteogenic layer away from the filter, 3) folded such that the osteogenic layer was in apposition with itself within the fold. The cultures were studied histologically as well as biochemically. Periostea that were cultured folded showed differentiation of osteoblastlike cells after 2 days, and production of osteoid at day 4. Tissues cultured with the osteogenic layer away from the filter demonstrated similar osteoblastic differentiation and osteoid production. Both types of cultures exhibited an increase in histochemically detectable alkaline phosphatase activity over the 4 day culture period that was associated with osteoblasts and the osteogenic area. Periostea cultured with the osteogenic layer facing the filter produced no osteoid. In these cultures, histochemically detectable alkaline phosphatase activity decreased and virtually disappeared over the 4 day culture period. The possibility that the creation of a suitable micro-environment is required for osteodifferentiation in this culture system is discussed.     

四种水力流变除栓装置体外除栓效果比较

目的　比较 6F、8FOasis和 6F、7FHydrolyser 4种流变血栓清除导管在模拟股静脉的体外流体模型中的除栓效果 ,注入盐水量及回收液体量 ,以及与除栓过程相关的远端栓塞率 .方法　用 3天的猪血在模拟股静脉的体外流体模型中形成约 2 0cm长的血栓栓塞 ,分别使用 6F、8FOasis除栓导管和 6F、7FHydrolyser除栓导管进行血栓清除 (每组10例 ) .结果　平均除栓时间从 30s( 7FHydrolyser)到 186s( 6FHydrolyser,p <0 .0 1) ;平均注入盐水量从 118ml( 7FHy drolyser)到 4 35ml( 6FHydrolyser,p <0 .0 1) ;注入盐水量与回收液体量的比值从 0 .6 6 ( 8FOasis)到 0 .80 ( 6FHydrolyser,p<0 .0 1) ;血栓清除率为 93.2 4 % ( 6FHydrolyser)到 97.2 1% ( 8FOasis,p <0 .0 1) ;除栓后远端微栓子形成率为 1.94 %( 8FOasis)到 3.5 7% ( 7FHydrolyser,p <0 .0 1) .结论　血栓清除时间和远端微栓子形成率与导管的选择有关 ;注入盐水量及回收液体量的比值在所有导管都可以接受 ;在模拟的股静脉模型中进行水力流变除栓大管径的导管 ( 7FHy drolyser和 8FOasis)是较优选择     

诺氟沙星注射液的体外抗菌活性研究

国产诺氟沙星注射液对四川地区临床分离出的330株致病菌(其中革兰氏阴性菌210株,革兰氏阳性菌120株)的体外抗菌活力与诺氟沙星片基本一致,均显示广谱、高效抗菌作用,对阴性菌的作用较强,尤其对痢疾杆菌、大肠杆菌、克氏肺炎杆菌的作用最强,MIC_(50)、MIC_(90)均≤0.5μg/ml;对肠道杆菌的抑菌活力与依诺沙星相似或略强,优于庆大霉素;对非产酶葡萄球菌的抑菌活力优于依诺沙星,与头孢曲松相同,MIC_(50)均为1.0μg/ml;对表皮葡萄球菌、链球菌、粪链球菌的作用均较依诺沙星、庆大霉索和头孢曲松强。不同的血清浓度(0～75％)、细菌接种量(10~4～10~8CFU/ml)及pH值(5.0～9.0)对其抗菌活性的影响不大。试验亦表明诺氟沙星注射液具强的杀菌作用。     

黄芩等6味中药对多重耐药鲍曼不动杆菌的体外抑菌活性的实验研究

目的:探究黄芩、黄连等6味中药材对多重耐药鲍曼不动杆菌(MDR-AB)的体外抑菌和杀菌活性。方法:选用黄芩、黄连、连翘、乌梅、穿心莲、金银花药材的提取物,采用药敏纸片琼脂扩散法及肉汤稀释法,测定提取物对MDR-AB的体外抑菌圈的最低抑菌浓度(MIC)。结果:黄芩、黄连提取物有较强的抑菌活性,抑菌圈直径大,MIC值较低;而乌梅提取物有一定的抑菌、杀菌活性,连翘、穿心莲、金银花提取物的抑菌活性相对较弱。结论:6味中药材的提取物对MDR-AB均有不同程度的抑菌活性,其中黄芩、黄连、乌梅3种提取物对10株MDR-AB的活性相对较高,而黄芩的抑菌效果为最好。     

严重自发空泡化对胚胎发育潜能的影响

目的:探讨授精后第5天(D5)严重自发空泡化的形成对胚胎发育潜能的影响。方法:回顾性分析2016年1月-2017年12月于本中心行体外受精-胚胎移植(IVF-ET)或卵细胞胞质内单精子注射术(ICSI)治疗患者的临床资料,选取授精后D5出现严重自发空泡化的873个胚胎作为空泡组,同期形态无异常的5709个胚胎作为对照组。比较两组第6天(D6)胚胎的可利用囊胚率、优质囊胚率及临床结局。结果:空泡组D6可利用囊胚率(8.3%)及优质囊胚率(22.2%)均低于对照组(27.9%、65.2%)(P<0.05)。临床结局方面,无论是单胚复苏移植还是与正常胚胎的联合复苏移植,空泡组与对照组的妊娠率、活产率和流产率均无差异(P>0.05)。结论:授精后D5严重自发空泡化的形成虽然影响D6可利用囊胚率及优质囊胚率,但可利用囊胚与对照组有相似的临床结局。     

治痔胶囊与槐角丸体外抑菌活性比较

目的 比较治痔胶囊与槐角丸的体外抑菌活性. 方法 采用琼脂稀释法,观察治痔胶囊与槐角丸溶液对6种常见细菌生长的抑制作用. 结果 治痔胶囊对常见的6种细菌均有抑菌作用,对金黄色葡萄球菌、变形杆菌、乙型链球菌的最低抑制浓度(minimal inhibitory concentration,MIC)为0.062 5 g&#8226;mL^-1,对铜绿假单胞菌的MIC为0.125 g&#8226;mL^-1,对大肠埃希菌的MIC为0.25 g&#8226;mL^-1,对肠球菌的的最低抑菌浓度为0.5 g&#8226;mL^-1.槐角丸对金黄色葡萄球菌、变形杆菌的生长有抑制作用,对两者的MIC均为0.125 g&#8226;mL^-1,槐角丸对另外4种细菌生长均无抑制作用. 结论 治痔胶囊比槐角丸具有更广的抗菌谱和更强的抑菌能力.