Development and characterization of DNAzyme candidates demonstrating significant efficiency against human rhinoviruses

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慢性胃炎脾气虚证与脾胃湿热证的差异表达基因比较

目的：比较慢性胃炎脾气虚证与脾胃湿热证患者差异表达基因。方法:分别提取慢性胃炎脾气虚和脾胃湿热患者胃黏膜组织RNA各4例，逆转录荧光探针标记后杂交制作BiostarH-140 s基因芯片。采用荧光值ratio、生物信息学、t检验等方法分析结果，实时荧光定量PCR检测部分相关基因。结果:获得差异表达基因245条，主要为营养物质消化吸收运输、物质能量合成代谢、细胞周期增殖分化、免疫反应等相关基因；有显著意义的差异表达基因77条；实时定量PCR检测10条基因，6条与芯片结果一致。结论:慢性胃炎脾气虚和脾胃湿热2个证型基因表达存在明显差异，提示中医的临床辨证分型与基因差异表达有一定关系。     

基于灰色关联分析的中医证候推理诊断方法

针对中医诊断专家系统存在的问题,通过模拟专家诊断的思维过程,引入征候筛选和灰色关联分析模型对诊断过程进行模拟和评价,实现了一个新的计算机化中医诊断方法。     

Die Klinik des hämolytisch-urämischen Syndroms

Zusammenfassung Neben dem sporadisch auftretenden HUS, das vorwiegend das Kleinkindesalter betrifft, ist die rezidivierende und die familiäre Form zu differenzieren. Außerdem kann bei Erwachsenen eine besondere Verlaufsform differenziert werden, bei Frauen nach Schwangerschaften oder unter Antikonzeptiva. Es erscheint sinnvoll diese Gruppierung vorzunehmen, da therapeutische Maßnahmen und Prognose stark variieren. Die unterschiedlichen Formen lassen kein übergeordnetes pathogenetisches Prinzip erkennen. Daher bleiben auch gezielte kausaltherapeutische Versuche unsicher. Gesichert kann gelten, daß Dialyse und die intensive Hypertonie-Behandlung die Prognose insbesonders bei den Kleinkindern entscheidend verbessert hat.     

中西医结合治疗腰椎管狭窄208例

目的探讨腰椎管狭窄临床治疗的方法。方法230例腰椎管狭窄病人，采用中两医结合治疗，治疗时间10～30天，平均20天，回顾性分析其治疗效果。结果经1～3年随访(平均20个月)，按照中华骨科学会脊柱外科的评定标准优62例(29．8％)，良127例(61％)，差19例(9．2％)，优良率90．8％。结论对于腰椎管狭窄症采用卧床休息，适当运动，合理锻炼，骶管疗法，中药的辨证施治等措施，结果表明，本方法为治疗腰椎管狭窄症的一种好方法。     

Identification of a family with nonspecific mental retardation (MRX79) with the A140V mutation in the MECP2 gene: Is there a need for routine screening?

Mutations in the methyl-CpG-binding protein 2 (MECP2) cause Rett syndrome, a severe neurodevelopmental disorder occurring predominantly in females. Male patients with Rett syndrome are extremely rare, as the Rett-causing mutations in the MECP2 gene are usually lethal in hemizygous males. However, different mutations in the same gene were reported to cause mental retardation, both in sporadic non-syndromic males as well as in syndromic families with disease manifestation in carrier females. The majority of the reported MECP2 mutations in mentally retarded patients cause amino acid substitutions and, especially in isolated cases, discrimination between a disease-causing mutation and a rare polymorphism is not obvious and the significance of each individual variation should be verified. We mapped a new non-syndromic X-linked family (MRX79) to the chromosomal region Xq27.3-Xq28 and identified an A140V mutation in the MEPC2 gene in all patients with the disease haplotype. In addition to data published by others, this suggests that A140V is a recurrent mutation (and not a polymorphism) found in patients with X-linked mental retardation.     

病历、电子病历与中医电子病历

本文从中医病历的历史演化，西医病历的发展过程出发，对中西医病历的特点进行了分析，并对电子病历及中医电子病历作了一些简单的介绍。     

A case of Lambert-Eaton myasthenic syndrome associated with atypical bronchopulmonary carcinoid tumor

The Lambert-Eaton myasthenic syndrome (LEMS) is typically recognized as a paraneoplastic syndrome associated with a small cell lung carcinoma (SCLC), whereas LEMS with other neuroendocrine lung tumors, including carcinoids or large cell lung carcinoma, are highly unusual. Here, we report a rare case of LEMS with atypical bronchopulmonary carcinoid tumor: a 65-yr-old man presented with progressive leg weakness and a diagnosis of LEMS was made by serial repetitive nerve stimulation test. Chest CT revealed a lung nodule with enlargement of paratracheal lymph nodes, and surgically resected lesion showed pathological features of atypical carcinoid tumor. We concluded that LEMS could be associated with rare pulmonary neuroendocrine tumor other than SCLC, which necessitates pathologic confirmation followed by aggressive treatment for optimal management in these rare cases.     

基于 Shapiro 模型推理算法的智能知识获取在中医知识库中的实现

本文应用现代计算机技术,将Shapiro模型推理算法运用于中医知识库中,使该知识库系统具有智能知识获取的能力.     

Ultrasonic vocalizations and maternal-infant interactions in a rat model of fetal alcohol syndrome

When isolated from their dams and littermates, rat pups emit ultrasonic vocalizations to elicit attention and retrieval from their dams. This study examined the effects of perinatal alcohol exposure on ultrasonic vocalizations and maternal-infant interactions. Alcohol was administered throughout gestation to the dams and during the early postnatal period to the pups. Control groups consisted of a nontreated control and an intubated, pair-fed control. Ultrasonic vocalizations were measured on postnatal day (PD) 5 under varying conditions of isolation. Maternal behaviors were examined on PD2, 4, 6, 8, and 10. Maternal behaviors were not significantly affected by prior alcohol administration to either the dams or the pups. However, ethanol-exposed rat pups vocalized more on PD5 than controls regardless of condition. The heightened vocalization response of the ethanol-exposed pups might be an underlying factor in the persistent effects of perinatal ethanol exposure on social behavior.