鼻渊舒口服液与西药治疗慢性鼻窦炎的临床疗效及安全性对比分析

目的研究并对比鼻渊舒口服液与西药治疗慢性鼻窦炎的临床疗效及安全性。方法 2013年1~12月该院门诊就诊的慢性鼻窦炎患者共186例。以数字法随机分成观察组(93例)和对照组(93例)。观察组患者使用鼻渊舒口服液进行治疗;对照组患者则使用西药进行治疗。对两组患者进行为期40d的治疗后,通过患者的治疗效果、用药后并发症以及治疗后的复发情况来比较两组患者的治疗情况。结果通过40d的治疗后发现观察组和对照组的临床疗效都比较好,两组的治疗效果差异无统计学意义(P0.05)。在治疗中因为用药导致全身多个系统出现并发症,观察组的总并发症发生率为4.3%,对照组30.11%,两组恶心呕吐、头晕目眩、全身乏力及总并发症发生率差异均有统计学意义(均P0.05)。在治疗结束后两组患者均出现不同程度上的病情复发,观察组有8例(8.60%)患者出现复发,而对照组中则有32例(34.41%)患者出现复发,两组复发率差异有统计学意义(P0.05)。结论使用鼻渊舒口服液对慢性鼻窦炎进行治疗后发现治疗结果良好,并发症少,值得在临床上广泛推广。     

四川川南地区321例宫颈细胞学ASCUS患者临床分析

目的:分析宫颈液基薄层细胞学TCT检测结果为未明确诊断意义的不典型鳞状上皮细胞(ASCUS)患者的临床与组织学诊断结果,探讨其临床意义及处理方式。方法:研究对象共321例,患者均来自四川川南地区,行宫颈液基细胞学检查且有组织病理学进行对照,应用TBS分级诊断标准。结果:321例ASCUS患者中,36~45岁年龄段检出率最高(48.6%)。病理活检结果为炎症占68.5%,CINⅠ级占13.7%,CINIⅡ级占8.7%,CINⅢ级占9.0%。其中,患者≤25岁CIN占37.5%,36~45岁CIN占37.8%,宫颈病变发病年龄出现低龄化的趋势。结论:四川川南地区,ASCUS患者隐藏着CIN病变,对待ASCUS患者应给予个体化处理,从而降低宫颈癌的发病率。     

Proteomic Analysis for Finding Serum Pathogenic Factors and Potential Biomarkers in Multiple Myeloma

Background:

Multiple myeloma (MM) is a malignant tumor, which takes the second place in malignant blood disease. The clinical symptoms are complicated that make more difficult to diagnose and therapy. Lots of researches focus on the proteins about MM in order to solve those problems. We used proteomic methods to find potential biomarkers in MM patients.

Methods:

We applied the peptide ligand library beads (PLLBs) to deplete high abundance proteins in serum for finding potential pathogenic factors and biomarkers of MM. Using 1D-Gel-liquid chromatography-tandem mass spectrometry (LC-MS/MS), we identified 789 and 849 unique serum proteins in MM patients and in healthy controls, respectively.

Results:

Twenty-two proteins were found differentially expressed between the two groups including serum amyloid A protein, vitamin D-binding protein isoform-1 precursor, plasma kallikrein, and apolipoprotein A-I. Changes of integrin alpha-11 and isoform-1 of multimerin-1 were validated with Western blotting. The linkage of the differentially expressed proteins and the pathogenesis pathways of MM were discussed.

Conclusions:

PLLB combined with 1D-gel-LC-MS/MS analysis is an efficient method to identify differentially expressed proteins in serum from patients with MM.     

相变型链霉亲和素化PLGA纳米粒的制备

目的 制备一种相变型链霉亲和素化的聚乳酸-羟基乙酸共聚物(PLGA)纳米粒(PLGA-SA/PFP),研究体外热致相变、声致相变增强超声显影的效果.方法 采用单乳化法及碳二亚胺法制备PLGA-SA/PFP,显微镜加热板法进行热致相变,低强度聚焦超声(LIFU)进行声致相变.结果 PLGA-SA/PFP的平均粒径为(346.2±74.49) nm,链霉亲和素结合量为2 208个分子/纳米粒.43.7℃时纳米粒发生相变,LIFU作用后可观察到超声显影增强.结论 成功制备了PLGA-SA/PFP,可用于预定位靶向实验.     

液体石蜡油预防俯卧位手术患者面部压疮的效果评价

目的：探讨液体石蜡油对预防术中皮肤压疮的应用效果。方法选取本院麻醉科2013年10月－2014年10月脊柱俯卧位手术患者356例,随机分为观察组及对照组各178例。对照组采用常规预防压疮护理措施：清洁患者面部皮肤,将患者受压处垫自制棉垫,保持面部受力均匀等。观察组在全麻气管插管成功后,取俯位卧前,用棉签蘸液体石蜡油涂抹在患者面部皮肤与头托的接触部位,以滚动法翻成俯卧位后,在前额、两侧颧弓等受压处垫上棉垫,观察患者面部与头架上的海绵垫接触部位是否受力均匀,有无压迫眼部,气管导管及气囊是否固定牢固,有无受压。观察记录术后面部皮肤受压情况。结果两组患者术后面部压疮发生率及压疮程度比较,差异有统计学意义（P＜0．05）。结论液体石蜡油可以有效地预防手术中患者面部压疮的发生,是一种安全、有效、操作简便的方法。     

康复新液对HPV阳性宫颈糜烂患者转阴的临床效果研究

目的：观察康复新液对HPV阳性患者转阴的治疗作用。方法对门诊治疗的宫颈糜烂患者,给予HPV筛查,对筛查阳性的162例患者,随机分为研究组和对照组,研究组给予康复新液阴道用药治疗12个月,对照组给予保妇康栓阴道用药12个月,比较治疗效果和HPV转阴情况。结果研究组治疗效果明显好于对照组,治疗12个月时,痊愈61例(75．31%),好转11例(13．58%),无效9例(11．11%),有效率88．89%;对照组治疗12个月时,痊愈36例(44．44%),好转12例(14．81%),无效33例(40．74%),有效率59．26%。研究组转阴36例(44．44%),显著改善32例(39．51%),改善5例(6．17%),无改善8例(9．88%),升高0例(0．00%),改善率90．12%;对照组转阴21例(25．93%),显著改善22例(27．16%),改善6例(7．41%),无改善31例(38．27%),升高1例(1．23%),改善率60．49%。结论康复新液用于治疗HPV阳性宫颈糜烂患者,不仅有利于病情更好恢复,而且对HPV转阴有临床价值,因而对宫颈糜烂发生癌变有预防作用。     

Liquid biopsy is a valuable tool in the diagnosis and management of lung cancer

Liquid biopsy refers to the use of various body fluids to test for circulating biological elements derived from the tumor. Liquid biopsy has taken on an increasingly important role in lung cancer diagnosis, molecular characterization, surveillance, monitoring, and determining mechanisms of resistance. These assays can utilize various sources of cell-free DNA (cfDNA) including blood, pleural fluid, urine, and others to detect tumor associated alterations. With the increasing power of next-generation sequencing technologies and the development of assays such as digital droplet PCR, rare tumor alleles can be detected in cfDNA to determine key characteristics of the tumor. Current assays, while effective, are still challenged by limited sensitivity and capacity to single genes or small panels of genes, though this is rapidly expanding. Nevertheless, testing of cfDNA has been shown to be valuable in detecting resistance to targeted inhibitors, particularly for detection of T790M in EGFR and monitoring response to therapy. With the continued development of more powerful and sensitive assays, these techniques will empower clinicians to better characterize early stage disease and can be used in the screening of high-risk patients, which may eliminate the requirement for tissue diagnosis in some settings. That said, since the majority of these alterations are not specific to lung cancer, there will continue to be a need for tissue in at least the initial diagnosis. Used in conjugation with tissue sampling, these assays will assist the treating clinician and the pathologist to better characterize individual tumors, even in the setting of limited tissue.     

清肺方离子导入治疗痰热壅肺型社区获得性肺炎疗效观察

目的 观察清肺方离子导入治疗痰热壅肺型社区获得性肺炎（community acquired pneumonia,CAP）的疗效。方法 将60例患者随机分为治疗组和对照组,每组30例,对照组采用常规抗感染、化痰治疗,治疗组在对照组治疗的基础上加清肺方离子导入治疗12 d;治疗前、治疗6 d后、治疗12 d后,分别观察两组患者中医证候积分、白细胞（white blood cell,WBC）计数、中性粒细胞百分比（neutrophil percentage,N%）、超敏C反应蛋白（high sensitivity C-reactive protein,hs-CRP）及胸部CT的变化情况。结果 与治疗前比较,两组患者治疗6、12 d后,中医证候积分、WBC计数、N%、hs-CRP水平均显著下降（P＜0.05）;治疗6、12 d后,治疗组患者中医证候积分、WBC计数均显著低于对照组（P＜0.05）。两组患者治疗后肺部炎症吸收情况比较,差异有统计学意义（P＜0.05）。结论 清肺方离子导入治疗痰热壅肺型CAP能够较快缓解患者咳嗽、咳痰、胸痛、发热等症状,促进肺部炎症吸收。     

Different metabolic profiles of K1 serotype and non-serotype K1 and K2 Klebsiella pneumoniae isolates in oral infection mice model

K1 or K2 serotype Klebsiella pneumoniae isolate caused clinical pyogenic liver abscess (KLA) infection is prevalent in many areas. It has been identified that K1 or K2 serotype K. pneumoniae isolates caused KLA infection in mice by oral inoculation. In our study, K1 serotype K. pneumoniae isolate Kp1002 with hypermucoviscosity (HV)-positive phenotype caused KLA infection in C57BL/6 mice by oral inoculation. Simultaneously, non-serotype K1 and K2 isolate Kp1014 with HV-negative phenotype failed to cause KLA infection in the same manner. It seems that gastrointestinal tract translocation is the pathway by which K1 or K2 serotype K. pneumoniae caused KLA infection. Liquid chromatography-tandem mass spectrometry was used to further analyze metabolic profile changes in mice with KLA infection. Data showed that after Kp1002 or Kp1014 oral inoculation, serum Phosphatidylcholine (PC) and Lysophosphatidylcholine (LPC) levels significantly changed in mice. Some PC and LPC molecules showed changes both in the Kp1002 KLA group and the Kp1014 no-KLA group compared with the control group. The level of 18:1/18:2-PC significantly changed in the Kp1002 KLA group compared with the control group, but showed no change between the Kp1014 no-KLA group and the control group. The level of 18:1/18:2-PC might have been particularly affected by KLA infection caused by K1 serotype K. pneumoniae Kp1002. It may be a potential biomarker for KLA infection.     

Thyroid hormonal profile in elderly patients treated with two different levothyroxine formulations: A single institute survey

PurposeRecent evidences suggest that, despite the large use of levothyroxine (L-T4), up to 40% of patients are over-treated developing sub-clinical hyperthyroidism. We compared TSH, fT4 and fT3 serum levels of elderly patients in treatment with liquid and tablet L-T4 formulations over a period of time of five years.SubjectsPatients were recruited by searching the database of those treated and followed at the Thyroid Unit of the University of Brescia.ResultsTwo hundred and ninety-nine patients (251 females, 48 males) were treated with L-T4 in tablet form (group T) and 118 subjects (107 female, 11 male) with liquid LT4 (group L). The two groups were super-imposable by age, median L-T4 dosage, TSH, fT4 and fT3 values. A slightly but not significantly higher BMI value was observed among patients of group L over those of group T (26.9 ± 2.9 vs. 26.4 ± 2.1 kg/cm², respectively). During five years of LT-4 treatment, sub-clinical or over-hypothyroidism was found in 13 (4.3%) and 3 (2.5%) patients of group T and group L (P = 0.335), whereas, subclinical or clinical hyperthyroidism was significantly more frequent among patients of group T than those of group L [69 (23%) vs. 5 (4.2%) patients, (P = 0.0001)]. Logistic regression analysis showed that only the Tablets were associated with the risk of developing subclinical or hyperthyroidism [OR 2·354 (1·136–4·827), P = 0.021].ConclusionsWe show a greater stability in the thyroid profile of hypothyroid elderly patients in treatment with liquid thyroxine as opposed to those being treated by tablet formulation over five years of follow-up.