GC测定白花前胡中白花前胡丙素和白花前胡丁素的含量

 目的建立了GC测定白花前胡根中白花前胡丙素(Pd-Ia)和白花前胡丁素（Pd-Ⅱ）含量的方法。方法采用SE-30石英毛细柱(0.22mm×25m),液膜厚度0.25um,FID检测器,以正二十五烷为内标物。结果Pd-Ia和Pd-Ⅱ的标准曲线分别在0.175～1.919ug (r=0.9995)和0.086～0.950ug(r=0.9996)之间有较好线性关系,Pd-I a和Pd-Ⅱ的加样回收率分别为(98.28±2.07)%和(99.58±2.44)%,并将CC和HPLC测定Pd-Ia,Pd-Ⅱ结果比较,两方法之间无显著性差异。结论本法简便,快速,准确,灵敏度高,对于前胡的质量检测有一定参考价值。     

HPLC法测定白花前胡中白花前胡丙素和紫花前胡中紫花前胡苷的含量

目的 :建立高效液相色谱法测定白花前胡根中白花前胡丙素 (dl-praeruptorinA ,Pd -Ia)和紫花前胡根中紫花前胡苷 (nodakenin ,NDK)含量的方法。方法 :色谱柱Shim -PackCLC -ODS ,Pd -Ia的流动相为甲醇 -水 (79∶2 1)和NDK的流动相为乙腈 -甲醇 -水 (2 0∶5∶75 ) ,流速为 1 0mL·min- 1 ,紫外检测波长分别为 32 3和 334nm。结果 :Pd -Ia及NDK的线性范围分别为 0 0 8～ 0 8μg和 0 0 1～ 0 10 μg ,相关系数均为 0 9999,平均回收率分别为 98 5 3%～ 99 2 7%和 97 6 0 %～99 87% ,RSD小于 2 %。结论 :方法准确 ,简便 ,快速 ,灵敏度高 ,对常用中药前胡的质量控制有较重要意义。     

西南地区习用前胡部分药理研究

对西南地区习用的云前胡、旱前胡和长前胡的试验结果表明：３种前胡的水、醇提取液均能增加小鼠呼吸道分泌，解除多种药引起的豚鼠气管平滑肌和家兔小肠平滑肌的痉挛，对正常小肠的自发性收缩抑制作用，云前胡水提物对整体动物还具有平喘作用，３种前胡的作用强度为：云前胡〉旱前胡〉长前胡，而且３种前胡的醇提物活性均较水提物强。     

Comparative research between Bai-hua Qian-hu, a Chinese traditional plant, and its active ingredient on nuclear factor-κB and tumor necrosis factor-α in isolated ischemia-reperfusion heart of rat

Ischemia-reperfusion (I/R) injury is a complicated pathophysiological process, which is involved in calcium overload, free radical production, metabolic abnormalities, and inflammatory reaction. With the development of modem molecular biology, the expression and regulation of gene in myocardial I/R injury arefocused on NF-κB.     

Absolute configuration determination of angular dihydrocoumarins from Peucedanum praeruptorum

From Peucedanum praeruptorum, one new khellactone ester (3'R)-O-acetyl-(4'S)-O-angeloylkhellactone (3), as well as four known angular dihydropyranocoumarins (1, 2, 4, 5) have been isolated. On the basis of NMR spectra and X-ray crystallography, their structures were determined. We have elucidated their absolute configuration by either chiral separation of their alkaline hydrolysis products with Rp-18 HPLC eluted with 5% hydroxypropyl-β-cyclodextrin (β-HCD) or by measurement of their CD spectra. A general rule relating the position and absolute stereochemistry of the khellactone esters to the sign of their Cotton effects in CD curves is proposed.     

河南与江西产白花前胡主成分药理作用比较研究

目的:比较江西和河南产白花前胡香豆素类主成分的药理作用。方法:鉴定主成分结构,比较镇咳、抗炎、祛痰作用。结果:2种植物主成分均有较好的镇咳、抗炎作用,祛痰作用均不明显。结论:河南产白花前胡与江西产白花前胡的香豆素类主成分有类似的功效。     

Inhibitory activity of monoamine oxidase by coumarins fromPeucedanum japonicum

Four coumarins were isolated from chloroform extract of the root of Peucedanum japonicum and identified as praeruptorin A(1), xanthotoxin (2), psoralen (3) and bergapten (4) on the basis of spectroscopic methods. The inhibitory activities of these coumarins on monoamine oxidase prepared by mouse brain were tested. The IC50 values of them were shown to be 27.4 microM (1), 40.7 microM (2), 35.8 microM (3), and 13.8 microM (4), in vitro.     

RP－HPLC分析白花前胡根和茎叶中花椒毒素的含量

建立了ＲＰ－ＨＰＬＣ分离和测定白花前胡根和茎叶中活性成分花椒毒素含量的方法，采用Ｃ＿（１８）柱，甲醇－水（６５：３５）为流动相，ＵＶ检测波长为２９７ｎｍ，花椒毒素在浓度为１～４μｇ／ｍｌ范围内（进样１μｌ），进样浓度（μｇ／ｍｌ）与峰面积呈直线相关，回归方程Ａ＝－０．１４１×１０￣３＋２．６１６×１０￣３Ｘ，ｒ＝０．９９９８。回收率为９９．９％，ＲＳＤ为０．６８％（ｎ＝５）。该方法步骤简便，重现性好，结果准确，分析速度快，可望成为分析白花前胡根和茎叶中花椒毒素含量的常规方法。     

RP-HPLC分析白花前胡根和茎叶中花椒毒素的含量

 建立了ＲＰ－ＨＰＬＣ分离和测定白花前胡根和茎叶中活性成分花椒毒素含量的方法，采用Ｃ＿（１８）柱，甲醇－水（６５：３５）为流动相，ＵＶ检测波长为２９７ｎｍ，花椒毒素在浓度为１～４μｇ／ｍｌ范围内（进样１μｌ），进样浓度（μｇ／ｍｌ）与峰面积呈直线相关，回归方程Ａ＝－０．１４１×１０￣３＋２．６１６×１０￣３Ｘ，ｒ＝０．９９９８。回收率为９９．９％，ＲＳＤ为０．６８％（ｎ＝５）。该方法步骤简便，重现性好，结果准确，分析速度快，可望成为分析白花前胡根和茎叶中花椒毒素含量的常规方法。     

大鼠急性心肌缺血-再灌注损伤时心肌超微结构的变化与白花前胡及前胡甲素的保护作用

目的研究大鼠急性心肌缺血 再灌注损伤时心肌超微结构的变化与白花前胡及前胡甲素的保护作用。方法利用大鼠在体心肌缺血再灌注模型 ,观察并对比研究了中药白花前胡 (PPD)及前胡甲素 (Pd Ia)对急性心肌缺血 再灌注损伤 (ischemiareperfusion ,IR)时心肌细胞结构的影响。结果白花前胡 (1 80mg·kg-1)和前胡甲素 (0 5mg·kg-1)能明显改善缺血再灌注所致的心肌光镜及电镜下细胞结构损伤。结论白花前胡和前胡甲素对大鼠急性心肌缺血 再灌注损伤时的心肌细胞有保护作用。